Synthesis of an 11-cis-locked biotinylated retinoid for sequestering 11-cis-retinoid binding proteins

2006 ◽  
Vol 84 (10) ◽  
pp. 1363-1370 ◽  
Author(s):  
Hiroko Matsuda ◽  
Shenglong Zhang ◽  
Andrea E Holmes ◽  
Sonja Krane ◽  
Yasuhiro Itagaki ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Membered Ring ◽  
Cross Linking ◽  
Retinoid Binding Proteins

The synthesis of a seven-membered ring locked analogue of 11-cis-retinol, tethered to a cross-linking moiety on C-15 and a lysine-extended biotin on the C-3, was accomplished for its utilization as a probe to fish out retinol binding proteins that may be involved in the reisomerization of retinal from all-trans to 11-cis in the visual cycle.Key words: retinoids, biotin, 11-cis-locked, cross-linking, retinol binding proteins.

scholarly journals Identification through chemical cross-linking of distinct granulocyte- macrophage colony-stimulating factor and interleukin-3 receptors on myeloid leukemic cells, KG-1

Blood ◽  
1989 ◽  
Vol 74 (8) ◽  
pp. 2652-2656 ◽  
Author(s):  
T Gesner ◽  
RA Mufson ◽  
KJ Turner ◽  
SC Clark
Keyword(s):  
Binding Proteins ◽  
Myelogenous Leukemia ◽  
Cross Linking ◽  
Colony Stimulating Factor ◽  
Interleukin 3 ◽  
Gm Csf ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor ◽  
Chemical Cross Linking

Abstract Granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) each bind specifically to a small number of high- affinity receptors present on the surface of the cells of the acute myelogenous leukemia line, KG-1. Through chemical cross-linking of IL-3 and GM-CSF to KG-1 cells, we identified distinct binding proteins for each of these cytokines with approximate molecular masses of 69 and 93 Kd, respectively. Although these two binding proteins are distinct, GM- CSF and IL-3 compete with each other for binding to KG-1 cells. Other cell lines, which express receptors for either factor but not for both do not display this cross-competition for binding with IL-3 and GM-CSF. These findings imply that distinct IL-3 and GM-CSF binding proteins are expressed on the cell surface and that an association exists between these proteins on KG-1 cells.

Novel Photo Affinity Cross-linking Resin for the Isolation of Heparin Binding Proteins

2000 ◽  
Vol 15 (6) ◽  
pp. 468-477 ◽  
Author(s):  
YASUO SUDA ◽  
MEGUMI NAKAMURA ◽  
SHUHEI KOSHIDA ◽  
SHOICHI KUSUMOTO ◽  
MICHAEL SOBEL
Keyword(s):  
Binding Proteins ◽  
Cross Linking ◽  
Heparin Binding
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