Polydisperse Mie theory applied to hollow latex spheres: An integrated light-scattering study

1992 ◽  
Vol 70 (6) ◽  
pp. 401-406 ◽  
Author(s):  
K. B. Strawbridge ◽  
F. R. Hallett
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Scattered Light ◽  
Mie Theory ◽  
Theoretical Models ◽  
Light Scattering Spectroscopy ◽  
Latex Spheres ◽  
Scattering Study ◽  
Computer Controlled ◽  
Sphere Suspensions

Integrated light-scattering spectroscopy provides a method of determining the size, coat thickness, and lumen refractive index of a variety of coated-sphere systems. Integrated light-scattering spectroscopy performed on hollow-latex-sphere suspensions also provides a basis for validating theoretical models, such as Rayleigh–Gans–Debye or Mie, of coated-sphere systems such as vesicles and micelles. The rigidity and stability of hollow latex spheres makes them an excellent candidate for the simulation of a "perfect" coated-sphere system and allows one to determine the effects of other nonstructural factors such as polydispersity. The computer controlled spectrometer collects data in the form of I(Q) vs. Q where I(Q) is the intensity of the scattered light. Mie theory, which provides an exact solution for the absorption and scattering of a sphere of arbitrary radius and refractive index, is used to fit the experimental data. The results are compared with data obtained from dynamic light scattering and electron microscopy.

Scattering patterns from thin optical fibres

1980 ◽  
Vol 58 (12) ◽  
pp. 1698-1702
Author(s):  
John W. Y. Lit
Keyword(s):  
Quality Control ◽  
Refractive Index ◽  
Light Scattering ◽  
Optical Fibre ◽  
Electromagnetic Waves ◽  
Scattered Light ◽  
Fibre Axis ◽  
Optical Fibres

The light scattering property of a thin optical fibre is studied theoretically. Plane polarized electromagnetic waves with their E-vector and H-vector parallel to the fibre axis are considered to fall perpendicularly on the fibre. Selected maxima and minima in the scattered light patterns are found to be very sensitive to the fibre parameters. The results may be used as a sensitive quality control of the fibre radius or refractive index while the fibre is being drawn.

Microscope laser light-scattering spectroscopy of vesicles within canaliculi of rat hepatocyte couplets

1995 ◽  
Vol 269 (1) ◽  
pp. G73-G84 ◽  
Author(s):  
G. M. Mockel ◽  
S. Gorti ◽  
R. K. Tandon ◽  
T. Tanaka ◽  
M. C. Carey
Keyword(s):  
Light Scattering ◽  
Laser Light ◽  
Scattered Light ◽  
Sodium Taurocholate ◽  
Bile Secretion ◽  
Laser Light Scattering ◽  
Rat Hepatocyte ◽  
Light Scattering Spectroscopy ◽  
Vesicle Secretion ◽  
Secretion Rates

Employing microscope laser light-scattering spectroscopy, we investigated "primary" bile secretion into canalicular spaces of rat hepatocyte couplets in monolayer culture. Time-dependent scattered light intensities were fitted by bi-exponential decays. The "slow" decay was attributed to an undulating canalicular membrane motion, whereas the "fast" decay was consistent with rapidly diffusing intracanalicular vesicles with mean hydrodynamic radii (+/- SD) of 479 +/- 53 A. After addition of micromolar concentrations of common bile salts, increases in the amplitude of the fast component facilitated a quantitative estimate of vesicle secretion rates. A dose-response relationship with 0.1-200 microM sodium taurocholate was characterized by an initial concentration-dependent increase and then a decrease in the amplitude of the fast canalicular component. Since these taurocholate concentrations are nontoxic to cultured hepatocytes, the maximum in vesicle-sized particles at 10 microM taurocholate suggested that its critical micellar concentration of approximately 5 mM was attained within the canalicular spaces. Sodium taurolithocholate resulted in time- and dose-dependent diminution in vesicle secretion rates, which after 2 h was followed by spontaneous canalicular recovery. This suggested that acute bicellular "cholestasis" was followed by oxidative metabolism and detoxification of the monohydroxy bile salt. Microscope laser light-scattering spectroscopy should facilitate further physical-chemical and pathophysiological studies of bile secretion at the cellular level.

scholarly journals Retrieving the refractive index of a sphere from the phase spectrum of its light-scattering profile

2021 ◽  
Vol 2015 (1) ◽  
pp. 012125
Author(s):  
A V Romanov ◽  
M A Yurkin
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Fourier Spectrum ◽  
Mie Theory ◽  
Phase Spectrum ◽  
Spectral Characterization ◽  
Single Particles ◽  
Homogeneous Sphere ◽  
Applicability Range ◽  
The Relationship

Abstract We studied the Fourier spectrum of the light-scattering profiles of single particles in the Rayleigh-Gans-Debye (RGD) and Wentzel–Kramers–Brillouin (WKB) approximations. In the case of a homogeneous sphere, we found the relationship between the key parameters of the spectrum (including its phase) and the sphere characteristics – both analytically and numerically in the framework of the approximations and the rigorous Lorentz–Mie theory, respectively. Based on these results, we have improved the existing spectral characterization method for spheres extending the applicability range to particles with a higher refractive index.

LIGHT-SCATTERING APPARATUS AND TECHNIQUES FOR MEASURING MOLECULAR WEIGHTS OF HIGH POLYMERS

1949 ◽  
Vol 27b (10) ◽  
pp. 791-806 ◽  
Author(s):  
H. J. Hadow ◽  
H. Sheffer ◽  
J. C. Hyde
Keyword(s):  
Molecular Weight ◽  
Refractive Index ◽  
Light Scattering ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Scattered Light ◽  
Molecular Weights ◽  
Sources Of Error ◽  
Reduced Intensity

Apparatus is described for the measurement of small amounts of scattered light, dissymmetry of radiation, depolarization, and very small refractive index differences. Methods of calibration are critically examined. Benzene was adopted as a turbidity standard. Measurements were made on fractionated polystyrene of known molecular weight and on bovine serum albumin solutions. Values of the reduced intensity for benzene were obtained:[Formula: see text]Degree and sources of error are discussed

scholarly journals Light scattering with stream-in-air flow systems.

1979 ◽  
Vol 27 (1) ◽  
pp. 264-267 ◽  
Author(s):  
G C Salzman ◽  
M E Wilder ◽  
J H Jett
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Air Flow ◽  
Mie Theory ◽  
Detector Response ◽  
Theoretical Base ◽  
Forward Angle ◽  
Scattering Measurements ◽  
Flow Systems ◽  
Cell Sizing

Both forward angle and 90 degrees light-scattering measurements have been used for cell sizing with stream-in-air flow systems with very little theoretical base for the measurements. Mie theory calculations are compared with measurements on plastic microspheres. Detector response for homogeneous spheres is shown to be sensitive to refractive index.

Phase transitions in C2O4HNH4, 1/2 H2O. A light scattering study at normal pressure

1987 ◽  
Vol 48 (5) ◽  
pp. 809-819 ◽  
Author(s):  
J.L. Godet ◽  
M. Krauzman ◽  
J.P. Mathieu ◽  
H. Poulet ◽  
N. Toupry
Keyword(s):  
Phase Transitions ◽  
Light Scattering ◽  
Normal Pressure ◽  
Scattering Study
Sign in / Sign up

Export Citation Format

Share Document