Isolation and characterization of a mannose-binding lectin from leaves of the Chinese daffodil Narcissus tazetta

1998 ◽  
Vol 76 (4) ◽  
pp. 601-608 ◽  
Author(s):  
Linda SM Ooi ◽  
Hexiang Wang ◽  
T B Ng ◽  
Vincent EC Ooi
Keyword(s):  
Gel Filtration ◽  
Deae Cellulose ◽  
Exchange Chromatography ◽  
Mannose Binding Lectin ◽  
Narcissus Tazetta ◽  
Isolation And Characterization ◽  
Mannose Binding ◽  
Narcissus Pseudonarcissus ◽  
Considerable Homology ◽  
Binding Lectin

A mannose-binding lectin was isolated from leaves of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae) using a procedure that comprised extraction with aqueous buffer, ammonium sulfate precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel Blue gel and mannose-agarose, and FPLC-gel filtration on Superose 12. The lectin was adsorbed on mannose-agarose and unadsorbed on DEAE-cellulose and Affi-gel Blue gel. It was an unglycosylated homodimer with a molecular mass of 26 kDa. Analysis of the N-terminal sequence of the N. tazetta lectin revealed considerable homology to lectins from the daffodil Narcissus pseudonarcissus, the snowdrop Galanthus nivalis (family Amaryllidaceae), the tulip Tulipa, and Kidachi aloe Aloe arborescens (family Liliaceae), and the orchid lectins (family Orchidaceae). The most striking likeness exists among the Amaryllidaceae lectins. The N. tazetta lectin exhibits hemagglutinating activity toward rabbit erythrocytes.Key words: daffodil, mannose-binding lectin, agglutinin.

A novel mannose-binding lectin from Liparis nervosa with anti-fungal and anti-tumor activities

2020 ◽  
Vol 52 (10) ◽  
pp. 1081-1092
Author(s):  
Na Jiang ◽  
Yuqing Wang ◽  
Jing Zhou ◽  
Ruxiao Zheng ◽  
Xiao Yuan ◽  
...  
Keyword(s):  
Gel Filtration ◽  
Sclerotium Rolfsii ◽  
Exchange Chromatography ◽  
Mannose Binding Lectin ◽  
Carbohydrate Binding ◽  
Plant Lectins ◽  
Mannose Binding ◽  
Anti Fungal ◽  
Binding Lectin

Abstract Plant lectins are carbohydrate-binding proteins with nonimmune origin, which can reversibly bind with carbohydrates, agglutinate cells, and precipitate polysaccharides and glycoconjugates. Plant lectins have attracted much attention for their anti-virus, anti-proliferation, and pro-apoptosis properties. Thus the exploration of new lectins has received special attention. Here we purified a mannose-binding lectin from the rhizomes of Liparis nervosa by ion exchange chromatography on DEAE-Sepharose, affinity chromatography on Mannose-Sepharose 4B, and gel filtration chromatography on Sephacryl S-100. The purified L. nervosa lectin (LNL) was identified to be a monomeric protein with a molecular mass of 13 kDa. LNL exhibited hemagglutinating activity towards rabbit erythrocytes, and its activity could be strongly inhibited by D-mannose, N-acetyl glucosamine and thyroglobulin. In vitro experiments showed that LNL exhibited a comparable anti-fungal activity against Piricularia oryzae (Cavara), Bipolaris maydis, Fusarium graminearum, and Sclerotium rolfsii, and anti-proliferation activity against tumor cells by inducing apoptosis. The full-length cDNA sequence of LNL is 715 bp in length and contains a 525 bp open reading frame (ORF) encoding a 110-residue mature protein. It was predicted to have three mannose-binding conserved motifs ‘QXDXNXVXY’. The binding pattern of LNL was further revealed by homology modeling and molecular docking. We demonstrated that LNL is not only a potential therapeutic candidate against tumor but also a new anti-fungal agent.

Lectins from bulbs of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae)

2000 ◽  
Vol 78 (4) ◽  
pp. 463-468 ◽  
Author(s):  
L SM Ooi ◽  
T B Ng ◽  
Yunqi Geng ◽  
V EC Ooi
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Syncytium Formation ◽  
Exchange Chromatography ◽  
Amino Acid Sequences ◽  
Lung Cells ◽  
Narcissus Tazetta ◽  
Mannose Binding ◽  
Fetal Bovine

The isolation of three lectins with similar N-terminal amino acid sequences from the bulbs of the Chinese daffodil Narcissus tazetta was achieved. The isolation protocol involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on mannose-agarose, and fast protein liquid chromatography-gel filtration on Superose 12. The lectins were all adsorbed on mannose-agarose and demonstrated a single band with a molecular weight of 13 kDa in SDS-polyacrylamide gel electrophoresis and a single 26 kDa peak in gel filtration, indicating that they were mannose-binding, dimeric proteins. The lectins differed in hemagglutinating activity, with the magnitude of the activity correlating with the ionic strength of the buffer required to elute the lectin from the DEAE-cellulose column. The bulb lectin did not exert potent cytotoxicity against cancer cell lines or fetal bovine lung cells but inhibited syncytium formation in, and reinstated viability of, fetal bovine lung cells infected with bovine immunodeficiency virus.Key words: lectins, daffodil bulbs, chromatography.

Isolation and characterization of a new mannose-binding lectin gene fromTaxus media

2004 ◽  
Vol 29 (4) ◽  
pp. 399-407 ◽  
Author(s):  
Guoyin Kai ◽  
Lingxia Zhao ◽  
Jingui Zheng ◽  
Lei Zhang ◽  
Zhiqi Miao ◽  
...  
Keyword(s):  
Mannose Binding Lectin ◽  
Lectin Gene ◽  
Isolation And Characterization ◽  
Mannose Binding ◽  
Binding Lectin

Isolation and characterization of a jacalin-related mannose-binding lectin from salt-stressed rice ( Oryza sativa ) plants

Planta ◽  
2000 ◽  
Vol 210 (6) ◽  
pp. 970-978 ◽  
Author(s):  
Willy J. Peumans ◽  
Annick Barre ◽  
Corinne Houles Astoul ◽  
Paula Rovira ◽  
Pierre Roug� ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Mannose Binding Lectin ◽  
Isolation And Characterization ◽  
Mannose Binding ◽  
Binding Lectin

scholarly journals Novel galactonic acid-binding hexameric lectin from Hibiscus mutabilis seeds with antiproliferative and potent HIV-1 reverse transcriptase inhibitory activities.

2009 ◽  
Vol 56 (4) ◽  
Author(s):  
Sze Kwan Lam ◽  
Tzi Bun Ng
Keyword(s):  
Reverse Transcriptase ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Inhibitory Activities ◽  
Binding Lectin ◽  
Hiv 1 ◽  
Hepatoma Hepg2 ◽  
Mcf 7

A hexameric 150-kDa lectin was isolated from dried Hibiscus mutabilis seeds using a chromatographic protocol that involved ion exchange chromatography on SP-Sepharose, and gel filtration on Superdex 75 and Superdex 200. The lectin was not adsorbed on SP-Sepharose and was eluted from the Superdex 75 column in the void volume. It was eluted in the first peak from Superdex 200. It was strongly adsorbed on DEAE-cellulose and Q-Sepharose and could not be easily desorbed. The hemagglutinating activity of the lectin, which was stable at pH 4-7 and up to 50 degrees C, could be inhibited by 25 mM galactonic acid. This is the first report of a galactonic acid-binding lectin. It potently inhibited HIV-1 reverse transcriptase with an IC(50) of 0.2 microM. It exhibited weak antiproliferative activity towards both hepatoma HepG2 cells (40% inhibition) and breast cancer MCF-7 cells (50% inhibition) at 100 microM concentration of the lectin. It did not inhibit mycelial growth of a number of fungi tested.

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