Accumulation of microtubule-associated protein 1A (MAP1A) in differentiating P19 embryonal carcinoma cells

1995 ◽  
Vol 73 (9-10) ◽  
pp. 695-702 ◽  
Author(s):  
A. R. Vaillant ◽  
D. L. Brown
Keyword(s):  
Neurite Outgrowth ◽  
Mitotic Spindle ◽  
Embryonal Carcinoma ◽  
Growth Cones ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Protein Levels ◽  
Undifferentiated Cells ◽  
P19 Embryonal Carcinoma Cells ◽  
Ec Cells

We have examined the accumulation of MAP1A in retinoic acid induced P19 embryonal carcinoma (EC) neurons. By immunofluorescent confocal microscopy, MAP1A was detected in the mitotic spindle of undifferentiated cells but was not evident in association with the interphase microtubules in most cells. By day 4 of differentiation, when neurite outgrowth was underway, MAP1A was co-localized with microtubules in all neurites but was absent from growth cones. By day 8, substantial neurite outgrowth had occurred and MAP1A was seen in all processes. At day 12, no further neurite outgrowth was evident and existing neurites were organized into fascicles. Western blotting and ELISA showed that MAP1A protein levels increased during differentiation. Peak accumulation occurred no later than day 8, coinciding with the period of neurite outgrowth, and then decreased after day 8. The results suggest that in differentiating P19 EC cells MAP1A modulates microtubule dynamics during neurite outgrowth.Key words: MAP1A, neuronal cytoskeleton, neurite outgrowth.

Effects of taxol on the polymerization and posttranslational modification of class III β-tubulin in P19 embryonal carcinoma cells

1995 ◽  
Vol 73 (9-10) ◽  
pp. 687-694 ◽  
Author(s):  
Nicole B. Laferrière ◽  
D. L. Brown
Keyword(s):  
Neurite Outgrowth ◽  
Posttranslational Modification ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Specific Class ◽  
P19 Cells ◽  
Embryonal Carcinoma Cells ◽  
Class Iii ◽  
P19 Embryonal Carcinoma Cells ◽  
Β Tubulin

Undifferentiated P19 embryonal carcinoma cells and P19 cells induced to differentiate along a neuronal pathway by 10−6 M retinoic acid were treated with taxol to examine the effects of this microtubule-stabilizing drug on the subcellular sorting of class III β-tubulin and on neurite outgrowth. P19 cells were grown on cover slips and then treated with taxol at concentrations of 10−6 to 10−9 M for 24 h. The microtubule cytoskeleton was examined after double-immunofluorescence labelling with a monoclonal antibody to α-tubulin (YOL 1/34) and a monoclonal neuron-specific class III β-tubulin antibody (TuJ1). Treatment of undifferentiated P19 cells with concentrations of taxol greater than 4 × 10−8 M caused microtubule bundling and multiple aster formation and promoted polymerization of the low levels of class III β-tubulin found in these cells. In neurons, at 2 × 10−8 M taxol, bundling of microtubules at the base of the neurite was apparent. At taxol concentrations greater than 1 × 10−7 M, enhanced assembly of class III β-tubulin was apparent, although long neurites were not observed. Using isoelectric focusing followed by western blotting, we detected an additional isoform of class III β-tubulin after treatment with 10−6 M taxol. These results indicate taxol treatment alters the normal subcellular sorting of tubulin isotypes, promotes the polymerization and posttranslational modification of class III β-tubulin, and interferes with neurite outgrowth.Key words: tubulin, taxol, microtubule, posttranslational modification, neurite outgrowth.

scholarly journals Synthesis and processing of small B2 transcripts in mouse embryonal carcinoma cells.

1990 ◽  
Vol 10 (8) ◽  
pp. 4058-4067 ◽  
Author(s):  
T S Bladon ◽  
C J Frégeau ◽  
M W McBurney
Keyword(s):  
Embryonal Carcinoma ◽  
Consensus Sequence ◽  
Rna Polymerase Iii ◽  
Cell Types ◽  
Nucleocytoplasmic Transport ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
B2 Rna ◽  
P19 Embryonal Carcinoma Cells ◽  
Rna Levels

B2 genes are short repeated sequences which are transcribed by RNA polymerase III. Abundant transcripts accumulate in embryonic and transformed cells, but transcripts are rare or absent from normal differentiated cell types. During retinoic acid-induced differentiation of P19 embryonal carcinoma cells, an early transient increase in B2 RNA levels is followed by a rapid drop in expression. The marked changes in B2 RNA levels are most likely due to transcriptional modulation since B2 RNA stabilities are unaffected by differentiation. At least four short-lived B2 RNAs with apparent lengths of 150, 180, 240, and 500 nucleotides were characterized. The two larger RNAs are polyadenylated and are more stable in cells. A cDNA of a B2 gene was isolated which was over 99% identical to the consensus sequence. This B2 cDNA can be transcribed in human cells and yields at least two distinct transcripts. We propose a model for B2 RNA metabolism which describes transcription, posttranscriptional modification and processing, and nucleocytoplasmic transport.

scholarly journals Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

2008 ◽  
Vol 53 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Izuho Hatada ◽  
Sumiyo Morita ◽  
Mika Kimura ◽  
Takuro Horii ◽  
Riu Yamashita ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Genome Wide ◽  
P19 Embryonal Carcinoma Cells

Neurogenesis Using P19 Embryonal Carcinoma Cells

10.3791/58225 ◽  
2019 ◽  
Author(s):  
Paweł Leszczyński ◽  
Magdalena Śmiech ◽  
Aamir S. Teeli ◽  
Aleksandra Zołocińska ◽  
Anna Słysz ◽  
...  
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells
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