Metastasis-associated alterations in phospholipids and fatty acids of human prostatic adenocarcinoma cell lines

1992 ◽  
Vol 70 (7) ◽  
pp. 548-554 ◽  
Author(s):  
Rajvir Dahiya ◽  
Bryan Boyle ◽  
Beth Carol Goldberg ◽  
Wan-Hee Yoon ◽  
Badrinath Konety ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Cell Lines ◽  
Cancer Metastasis ◽  
Prostatic Adenocarcinoma ◽  
Gas Liquid Chromatography ◽  
Arachidonic Acid Level ◽  
Lncap Cells ◽  
Type Iv Collagenase ◽  
Adenocarcinoma Cell

Metastatic variants of human prostatic adenocarcinoma cell lines (DU-145, LNCaP, and ND-1) were studied by using soft agar colony forming efficiency, nude mice tumorigenicity, in vitro invasion assay, and type IV collagenase assay. The DU-145 and ND-1 cell line snowed higher metastatic potential than LNCaP. Lipids from DU-145, ND-1, and LNCaP cells were extracted and analyzed by thin-layer chromatography and gas-liquid chromatography. The major lipids were phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, fatty acids, and cholesterol. The sphingomyelin level was significantly higher in highly metastatic cells (DU-145 and ND-1) compared with the lower metastatic variant (LNCaP). The increase in the synthetic pathway and decrease in degradation pathway of sphingomyelin in microsomal fractions was sufficient to account for the measured increase in sphingomyelin in DU-145 cells compared with LNCaP cells. The major fatty acids of these lipids were palmitic (16:0), stearic (18:0), oelic (18:1), and arachidonic acid (20:4). The arachidonic acid level was significantly decreased in DU-145 and ND-1 compared with LNCaP cells. Electron microscopic studies showed no significant changes in the morphology of DU-145, ND-1, and LNCaP cells. The results of these investigations demonstrate for the first time that sphingomyelin and arachidonic acid contents are different in high and low metastatic variants of human prostatic adenocarcinoma cell lines.Key words: prostate cancer, metastasis, morphology, phospholipids, cytogenetic.

scholarly journals The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

2020 ◽  
Author(s):  
Carla Ferreri ◽  
Anna Sansone ◽  
Sandra Buratta ◽  
Lorena Urbanelli ◽  
Eva Costanzi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Extracellular Vesicles ◽  
Cell Membranes ◽  
Saturated Fatty Acids ◽  
Prostatic Adenocarcinoma ◽  
Fatty Acid Isomer ◽  
Adenocarcinoma Cell

A new pathway leading to the n-10 fatty acid series has been recently evidenced, starting from sapienic acid - a monounsaturated fatty acid (MUFA) resulting from the transformation of palmitic acid by delta-6 desaturase. Sapienic acid attracts attention as novel marker of cancer cell plasticity. Here, we analyzed fatty acids including the n-10 fatty acid contents, and compared for the first time cell membranes and the corresponding extracellular vesicles (EV) of two human prostatic adenocarcinoma cell lines of different aggressiveness (PC3 and LNCaP). The n-10 components were 9-13% of the total fatty acids in both cancer cell lines and EVs, with total MUFA levels significantly higher in EVs of the most aggressive cell type (PC3). High sapienic/palmitoleic ratios indicated the preference for delta-6 vs. delta-9 desaturase enzymatic activity in these cell lines. The expressions analysis of enzymes involved in desaturation and elongation by qRT-PCR showed a higher desaturase activity in PC3 and a higher elongase activity toward polyunsaturated fatty acids than toward saturated fatty acids, compared to LNCaP cells. Our results improve the present knowledge in cancer fatty acid metabolism and lipid phenotypes, highlighting EV lipidomics to monitor positional fatty acid isomer profiles and MUFA levels in cancer.

scholarly journals The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 900 ◽  
Author(s):  
Carla Ferreri ◽  
Anna Sansone ◽  
Sandra Buratta ◽  
Lorena Urbanelli ◽  
Eva Costanzi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Extracellular Vesicles ◽  
Cell Membranes ◽  
Saturated Fatty Acids ◽  
Prostatic Adenocarcinoma ◽  
Fatty Acid Isomer ◽  
Adenocarcinoma Cell

A new pathway leading to the n-10 fatty acid series has been recently evidenced, starting from sapienic acid, a monounsaturated fatty acid (MUFA) resulting from the transformation of palmitic acid by delta-6 desaturase. Sapienic acid has attracted attention as a novel marker of cancer cell plasticity. Here, we analyzed fatty acids, including the n-10 fatty acid contents, and for the first time, compared cell membranes and the corresponding extracellular vesicles (EV) of two human prostatic adenocarcinoma cell lines of different aggressiveness (PC3 and LNCaP). The n-10 components were 9–13% of the total fatty acids in both cancer cell lines and EVs, with total MUFA levels significantly higher in EVs of the most aggressive cell type (PC3). High sapienic/palmitoleic ratios indicated the preference for delta-6 versus delta-9 desaturase enzymatic activity in these cell lines. The expressions analysis of enzymes involved in desaturation and elongation by qRT-PCR showed a higher desaturase activity in PC3 and a higher elongase activity toward polyunsaturated fatty acids than toward saturated fatty acids, compared to LNCaP cells. Our results improve the present knowledge in cancer fatty acid metabolism and lipid phenotypes, highlighting EV lipidomics to monitor positional fatty acid isomer profiles and MUFA levels in cancer.

scholarly journals The Effect of Fatty Acids on Ciprofloxacin Cytotoxic Activity in Prostate Cancer Cell Lines. Does Lipid Component Enhance Anticancer Ciprofloxacin Potential?

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 409
Author(s):  
Alicja Chrzanowska ◽  
Wioletta Olejarz ◽  
Grażyna Kubiak-Tomaszewska ◽  
Andrzej K. Ciechanowicz ◽  
Marta Struga
Keyword(s):  
Prostate Cancer ◽  
Fatty Acids ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Cancer Cell Lines ◽  
Lncap Cells ◽  
Ic50 Values ◽  
Late Apoptosis ◽  
Pc3 Cells

Purpose: To assess cytotoxic effect of ciprofloxacin conjugates with fatty acids on prostate cancer cells (LNCaP and DU-145) with different hormone sensitivity, based on previous promising results from the PC3 cells. Methods: Cytotoxicity were estimated using MTT and LDH tests, whereas its mechanisms were estimated by apoptosis and IL-6 assays. The intensity of proteins involved in lipid metabolism was determined using ML-CS assay. Results: The hormone insensitive DU-145 cells were more vulnerable than the hormone sensitive LNCaP cells. The IC50 values for oleic (4), elaidic (5) and docosahexaenoic acid (8) conjugates were 20.2 µM, 17.8 µM and 16.5 µM, respectively, in DU-145 cells, whereas in LNCaP cells IC50 exceeded 20 µM. The strong conjugate cytotoxicity was confirmed in the LDH test, the highest (70.8%) for compound (5) and 64.2% for compound (8) in DU-145 cells. This effect was weaker for LNCaP cells (around 60%). The cytotoxic effect of unconjugated ciprofloxacin and fatty acids was weaker. The early apoptosis was predominant in LNCaP while in DU-145 cells both early and late apoptosis was induced. The tested conjugates decreased IL-6 release in both cancer cell lines by almost 50%. Proteomic analysis indicated influence of the ciprofloxacin conjugates on lipid metabolic proteins in prostatic cancer. Conclusion: Our findings suggested the cytotoxic potential of ciprofloxacin conjugates with reduction in proteins involved in prostate cancer progress.

scholarly journals Prediagnostic plasma polyunsaturated fatty acids and the risk of amyotrophic lateral sclerosis

Neurology ◽  
2019 ◽  
Vol 94 (8) ◽  
pp. e811-e819 ◽  
Author(s):  
Éilis J. O'Reilly ◽  
Kjetil Bjornevik ◽  
Jeremy D. Furtado ◽  
Laurence N. Kolonel ◽  
Loic Le Marchand ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Amyotrophic Lateral Sclerosis ◽  
Arachidonic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Conditional Logistic Regression ◽  
Gas Liquid Chromatography ◽  
Blood Draw ◽  
Total N ◽  
Bottom Quartile ◽  
Lateral Sclerosis

ObjectiveTo examine the association between prediagnostic plasma polyunsaturated fatty acids levels (PUFA) and amyotrophic lateral sclerosis (ALS).MethodsWe identified 275 individuals who developed ALS while enrolled in 5 US prospective cohorts, and randomly selected 2 controls, alive at the time of the case diagnosis, matched on cohort, birth year, sex, ethnicity, fasting status, and time of blood draw. We measured PUFA, expressed as percentages of total fatty acids, using gas liquid chromatography and used conditional logistic regression to estimate risk ratios (RR) and 95% confidence intervals (CI) for the association between PUFA and ALS.ResultsThere was no association between total, n-3, and n-6 PUFA, eicosapentaenoic acid, or docosapentaenoic acid levels and ALS. Higher plasma α-linolenic acid (ALA) in men was associated with lower risk of ALS in age- and matching factor-adjusted analyses (top vs bottom quartile: RR = 0.21 [95% CI 0.07, 0.58], p for trend = 0.004). In women, higher plasma arachidonic acid was associated with higher risk (top vs bottom quartile: RR = 1.65 [95% CI 0.99, 2.76], p for trend = 0.052). Multivariable adjustment, including correlated PUFA, did not change the findings for ALA and arachidonic acid. In men and women combined, higher plasma docosahexaenoic acid (DHA) was associated with higher risk of ALS (top vs bottom quartile: RR = 1.56 [95% CI 1.01, 2.41], p for trend = 0.054), but in multivariable models the association was only evident in men.ConclusionsThe majority of individual PUFAs were not associated with ALS. In men, ALA was inversely and DHA was positively related to risk of ALS, while in women arachidonic acid was positively related. These findings warrant confirmation in future studies.

scholarly journals The use of Raman spectroscopy to differentiate between different prostatic adenocarcinoma cell lines

2005 ◽  
Vol 92 (12) ◽  
pp. 2166-2170 ◽  
Author(s):  
P Crow ◽  
B Barrass ◽  
C Kendall ◽  
M Hart-Prieto ◽  
M Wright ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cell Lines ◽  
Prostatic Adenocarcinoma ◽  
Adenocarcinoma Cell

5-Azacytidine modulates γ-glutamyltransferase and glutathione levels in two human prostatic adenocarcinoma cell lines

IUBMB Life ◽  
1998 ◽  
Vol 46 (2) ◽  
pp. 321-331
Author(s):  
M. El Yaagoubi ◽  
A. Visvikis ◽  
A. Khalid ◽  
G. Siest ◽  
M. Wellman
Keyword(s):  
Cell Lines ◽  
Prostatic Adenocarcinoma ◽  
Adenocarcinoma Cell
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