Expression of gene-specific RNA in cultured cells exposed to rotating 60-Hz magnetic fields

1992 ◽  
Vol 70 (3-4) ◽  
pp. 237-241 ◽  
Author(s):  
Jill E. Parker ◽  
Wendell Winters
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Cultured Cells ◽  
Sine Wave ◽  
Repeat Sequence ◽  
Dot Blot ◽  
Rotating Magnetic Fields ◽  
Murine Sarcoma Virus ◽  
Tumor Virus ◽  
Sarcoma Virus

Replicate sets of cultures of mouse and of human cells were exposed to cyclic, sine wave, 60-Hz rotating magnetic fields of 1.0 Gs [Formula: see text] for 24-, 48-, or 72-h periods. Total RNA extracted from unexposed control and from magnetic field exposed cells was dot blot hybridized to a number of oncogene probes (including v-myc, v-fos, v-raf, and v-Ha-ras), a probe for 3611 murine sarcoma virus, a probe for the 70 000 dalton heat shock protein (hsp70), and a probe for the long terminal repeat sequence of mouse mammary tumor virus. Comparisons of levels of RNA in unexposed and magnetic field exposed cells measured by densitometer readings of resulting autoradiographs revealed no significant increases or decreases in RNA levels in magnetic field exposed cells with the seven probes tested.Key words: 60 hertz, oncogene, mRNA.

scholarly journals The hardening of metals by rotating magnetic fields

1931 ◽  
Vol 130 (814) ◽  
pp. 514-523 ◽  
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Work Hardening ◽  
Rotating Magnetic Fields ◽  
Hardening Process ◽  
Hard Steel ◽  
Steel Balls ◽  
Hardened Condition

In a previous paper by the author experiments were described in which the hardness of various metals was increased by rotating them in a magnetic field. It had been observed that metals in a work-hardened condition, and in particular hard steel which had been super-hardened by the “Cloudburst” process of bombardment with steel balls, exhibit a propensity to become still harder by a process of ageing, the spontaneous increase of hardness commencing with the termination of the work-hardening process, and contiuning during a period of several hours or days.

Frequency-dependent conversion of the torque of a rotating magnetic field on a ferrofluid confined in a spherical cavity

Soft Matter ◽  
2019 ◽  
Vol 15 (44) ◽  
pp. 9018-9030
Author(s):  
Klaus D. Usadel ◽  
Anastasiya Storozhenko ◽  
Igor Arefyev ◽  
Hajnalka Nádasi ◽  
Torsten Trittel ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Magnetic Fields ◽  
Spherical Cavity ◽  
Rotating Magnetic Field ◽  
Rotating Magnetic Fields ◽  
Frequency Dependent

The dynamics of magnetic nanoparticles in rotating magnetic fields is studied both experimentally and theoretically.

Solidification of AlSi Alloys in the ARTEMIS and ARTEX Facilities Including Rotating Magnetic Fields – A Combined Experimental and Numerical Analysis

2006 ◽  
Vol 508 ◽  
pp. 199-204 ◽  
Author(s):  
Marc Hainke ◽  
Sonja Steinbach ◽  
Johannes Dagner ◽  
Lorenz Ratke ◽  
Georg Müller
Keyword(s):  
Magnetic Field ◽  
Numerical Modeling ◽  
Numerical Analysis ◽  
Temperature Gradient ◽  
Magnetic Fields ◽  
Time Dependent ◽  
Flow Conditions ◽  
Rotating Magnetic Fields ◽  
Microstructural Parameters ◽  
Wide Range

The solidification microstructure is the consequence of a wide range of process parameters, like the growth velocity, the temperature gradient and the composition. Although the influence of these parameters is nowadays considerably well understood, an overall theory of the influence of convection on microstructural features is still lacking. The application of time dependent magnetic fields during directional solidification offers the possibility to create defined solidification and flow conditions. In this work, we report about solidification experiments in the ARTEMIS and ARTEX facilities including rotating magnetic fields (RMF). The effect of the forced melt flow on microstructural parameters like the primary and secondary dendrite arm spacing is analyzed for a wide range of magnetic field parameters. The experimental analysis is supported by a rigorous application of numerical modeling. An important issue is hereby the prediction of the resulting macrosegregation, i.e., differences in the composition on the scale of the sample (macroscale) due to the RMF. For the considered configuration and parameters an axial enrichment of Si is found beyond a certain magnetic field strength. The results are compared to available theories and their applicability is discussed.

Control of the Macrosegregations during Solidification of a Binary Alloy by Means of a AC Magnetic Field

2006 ◽  
Vol 508 ◽  
pp. 163-168 ◽  
Author(s):  
Xiao Dong Wang ◽  
A. Ciobanas ◽  
Florin Baltaretu ◽  
Anne Marie Bianchi ◽  
Yves Fautrelle
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Mushy Zone ◽  
Binary Alloy ◽  
Solidification Front ◽  
Laminar Flows ◽  
Rotating Magnetic Field ◽  
Moderate Intensity ◽  
Mushy Region ◽  
Rotating Magnetic Fields

A numerical model aimed at simulating the segregations during the columnar solidification of a binary alloy is used to investigate the effects of a forced convection. Our objective is to study how the segregation characteristics in the mushy zone are influenced by laminar flows driven both by buoyancy and by AC fields of moderate intensity. Various types of magnetic fields have been tested, namely travelling, rotating magnetic field and slowly modulated electromagnetic forces. The calculations have been achieved on two types of alloys, namely tin-lead and aluminiumsilicon. It is shown that the flow configuration changes the segregation pattern. The change comes from the coupling between the liquid flow and the top of the mushy zone via the pressure distribution along the solidification front. The pressure difference along the front drives a mush flow, which transports the solute in the mushy region. Another interesting type of travelling magnetic field has been tested. It consists of a slowly modulated travelling magnetic field. It is shown that in a certain range of values of the modulation period, the channels are almost suppressed. The normal macrosegregation remains, but the averaged segregation in the mushy zone is weaker than in the natural convection case. The optimal period depends on the electromagnetic force strength as well as the cooling rate. The latter phenomenon cannot occur in the case of rotating magnetic fields, since in that configuration the sign of the pressure gradient along the solidification front remains unchanged. Recent solidification experiments with electromagnetic stirring confirm the predicted macrosegregation patterns.

Rheology and Behavior of Magnetic Fluids in Alternating/Rotating Magnetic Fields

2003 ◽  
Author(s):  
Carlos Rinaldi ◽  
Xiaowei He ◽  
Adam Rosenthal ◽  
Thomas Franklin ◽  
Cory Lorenz ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Magnetic Fluids ◽  
Rotating Magnetic Fields ◽  
Magnetic Field Rotation ◽  
Three Phase ◽  
Field Rotation ◽  
And Behavior ◽  
The Magnetic Field ◽  
Cylindrical Test

The rheology and behavior of magnetic fluids in the presence of time-varying magnetic fields is illustrated through three sets of experiments. The first involves measurements of ferrofluid torque on a cylindrical spindle under applied uniform rotating magnetic fields. We measure the torque required to restrain a stationary cylindrical test wall in contact with aqueous ferrofluids subjected to the rotating uniform magnetic field generated by a three-phase AC 2-pole motor stator winding. The torque is found to scale linearly with volume, and to be a function of the applied magnetic field amplitude, frequency and direction of rotation. Measurements show that for ferrofluid entirely inside the cylindrical test wall the torque points in the same direction as the magnetic field rotation pseudovector, whereas for ferrofluid entirely outside the cylindrical wall the torque points in the direction opposite to the field rotation pseudovector. The second set of experiments explores the formation of ordered ferrofluid structures in the gap of a Hele-Shaw cell subjected to simultaneous vertical DC and in-plane horizontal rotating magnetic fields. Finally, the third set of experiments illustrates the effect of applied DC fields on the shape of ferrofluid jets and sheets.

Studies on the Acid Mucopolysaccharide Coat on Viral Envelopes and Transformed Cell Surfaces

1970 ◽  
Vol 28 ◽  
pp. 172-173
Author(s):  
T. Shigematsu ◽  
W. C. Williams ◽  
M. L. Lewis ◽  
R. L. Hales ◽  
L. Dmochowski
Keyword(s):  
Cell Walls ◽  
Cultured Cells ◽  
Staining Technique ◽  
Ruthenium Red ◽  
Acid Mucopolysaccharide ◽  
Specific Staining ◽  
Rous Sarcoma ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Viral Envelopes

It has been reported that cell walls transformed by SV-40 virus (Martinez-palomo,A.,et al.:Cancer Res.,29;925,1969; vorbrodt, A. and Koprowski,H.:J.Nat.Cancer Inst.,43;1241,1969)or Rous sarcoma virus (Morgan,H.R.;J.Virology,2;1133,1968) appear thicker in cross section and more diffuse than normal cell walls following staining with ruthenium red. The Luft method (Luft,J.H.: Proc. 6th Internat.Cong.for Electron Microscopy,2:65,1966), a specific staining technique for acid mucopolysaccharide (AMS) with ruthenium red, has been applied to cultured cells. Cells cultured from NB rat bone tumors induced by Soehner-Dmochowski murine sarcoma virus (SD-MSV) and from RIII strain spontaneous mouse mammary tumors were studied to characterize the nature of virion envelopes. The AMS coat adhering to both type C and type B particles was generally 100 to 200A thick (Fig. 1).

The Aharanov-Bohm Phase Shift and Magnetic Vector Potential “A” Could Accommodate for Optical Coupler, Digital-to-Analogue Magnetic Field Excess Correlations of Photon Emissions Within Living Aqueous Systems

2016 ◽  
pp. 3333-3340
Author(s):  
Michael A Persinger ◽  
Stanley A Koren
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Single Photon ◽  
Rest Mass ◽  
Radioactive Decay ◽  
Local Magnetic Field ◽  
Magnetic Vector Potential ◽  
Aqueous Systems ◽  
Magnetic Field Effects ◽  
Rotating Magnetic Fields

Quantitative convergence for solutions involving electron drift velocity, the magnetic A vector and phase shifts reveal an increment of energy in the range of 10-20 J that could relate the Aharanov-Bohm phase modulation of the orbital frequency of a Bohr atom to the electron’s Compton wavelength. The universal persistence of 10-12 W per m2 whose energy applied the square of the hydrogen wavelength solves for the energy equivalence of the rest mass of an electron could set the conditions for excess correlations between electronic systems that produce magnetic fields through optocouplers. Experimental evidence and quantitative solutions indicate variations of the Lorentz Lemma and circularly rotating magnetic fields whose phase and group velocities are uncoupled could create the conditions for excess correlations. Modification of Basharov’s operator of resonance interaction for decoherence and entanglement in the radioactive decay of a diatomic system and Das and Misra’s estimates for the fractal charge of a photon strongly suggests that the efficacy for optocoupler circuits to generate non-local magnetic field effects in living and non-living aqueous systems originates from a single photon wave across the circuit’s p-n junctions. A review of the concepts and data indicate that excess correlations involving photons under optimal conditions are measureable within macrosystems

Markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line

1981 ◽  
Vol 1 (1) ◽  
pp. 66-74
Author(s):  
E M Scolnick ◽  
M O Weeks ◽  
T Y Shih ◽  
S K Ruscetti ◽  
T M Dexter
Keyword(s):  
Cell Line ◽  
Gene Product ◽  
Cultured Cells ◽  
Precursor Cell ◽  
Hemopoietic Stem Cell ◽  
Guanine Nucleotide Binding Protein ◽  
Precursor Cell Line ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma

The src gene product of Harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. We have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the Harvey murine sarcoma virus-coded p21. In this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416B, which was originally described as a continuous cell line of a hemopoietic stem cell, CFU-S. The currently available 416B cells express markedly elevated levels of endogenous p21. The level of endogenous p21 in the 416B cells is 5- to 10-fold higher than the level of p21 in Harvey murine sarcoma virus-infected cells and more than 100 times higher than the level of endogenous p21 that we have observed in a variety of other fresh or cultured cells. The results indicate that marked regulation of the levels of an endogenous sarc gene product can occur, and speculation about a possible role for endogenous p21 in normal hemopoietic stem cells is discussed.

Semiconductor Crystal Growth by the Vertical Bridgman Process With Transverse Rotating Magnetic Fields

2006 ◽  
Vol 129 (2) ◽  
pp. 241-243 ◽  
Author(s):  
X. Wang ◽  
N. Ma
Keyword(s):  
Magnetic Field ◽  
Crystal Growth ◽  
Magnetic Fields ◽  
Applied Magnetic Field ◽  
Rotating Magnetic Field ◽  
Semiconductor Crystal ◽  
Rotating Magnetic Fields ◽  
Electrically Conducting ◽  
Vertical Bridgman ◽  
Bridgman Process

During the vertical Bridgman process, a single semiconductor crystal is grown by the solidification of an initially molten semiconductor contained in an ampoule. The motion of the electrically conducting molten semiconductor can be controlled with an externally applied magnetic field. This paper treats the flow of a molten semiconductor and the dopant transport during the vertical Bridgman process with a periodic transverse or rotating magnetic field. The frequency of the externally applied magnetic field is sufficiently low that this field penetrates throughout the molten semiconductor. Dopant distributions in the crystal are presented.

Ferrohydrodynamics in Time-Varying Magnetic Fields

2002 ◽  
Author(s):  
Carlos Rinaldi ◽  
June-Ho Lee ◽  
Adam D. Rosenthal ◽  
Thomas Franklin ◽  
Markus Zahn
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Outer Wall ◽  
Anomalous Behavior ◽  
Rotating Magnetic Fields ◽  
Free Surfaces ◽  
Couette Viscometer ◽  
Three Phase ◽  
Viscous Torque ◽  
Theoretical Analyses

Measurements of magnetic-field-induced ferrofluid flow and torque in uniform rotating magnetic fields are presented and compared to theoretical analyses in order to understand observed paradoxical behavior. The viscous torque from this fluid flow is measured using a cylindrical Couette viscometer, as a function of magnetic field amplitude, frequency, and direction of rotation. The first set of experiments measures the torque on the outer wall of a polycarbonate spindle that is attached to a viscometer, which functions as a torque meter. The spindle is immersed in the ferrofluid, which is centered in the gap of a three-phase AC 2-pole motor stator winding. Anomalous behavior, such as negative effective viscosity, is demonstrated and discussed. The second set of experiments measures the viscous torque on the inner wall of a hollow spindle attached to the torque meter and filled completely with ferrofluid so that there is no free surface. These measurements show that magnetic fluid effects arise even in the absence of free surfaces. These observations are then shown to agree with a recently derived analysis of spin-up flow in ferrofluids.

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