Purification and characterization of plasma T-kininogen from Wistar and brown Norway rats

1989 ◽  
Vol 67 (2-3) ◽  
pp. 86-92 ◽  
Author(s):  
Albert Adam ◽  
Jacques Damas ◽  
Claire Renard ◽  
Germain Calay ◽  
Victor Bourdon
Keyword(s):  
Amino Acid ◽  
Cysteine Proteinase ◽  
Sugar Content ◽  
Gel Filtration ◽  
Rat Plasma ◽  
Brown Norway ◽  
Cysteine Proteinase Inhibitor ◽  
Neutral Sugar ◽  
Norway Rat ◽  
Norway Rats

A rapid and convenient three-step purification scheme has been developed for the purification of T-kininogen (alpha1-cysteine proteinase inhibitor) from rat plasma. The purification process includes chromatography on hydroxyapatite, immunoaffinity chromatography and gel filtration. This procedure is applied to plasma from the brown Norway rat which is known to be deficient in high and low molecular weight kininogens. The method furnished large amounts of T-kininogen from turpentine-treated Wistar rats as well as from untreated and turpentine-treated deficient brown Norway rats. The amino acid and hexose content of the three T-kininogens has been determined. While the composition of the molecules isolated from both injured rats was similar, the neutral sugar content of T-kininogen purified from untreated brown Norway rats was lower and its amino acid composition showed slight differences. The three molecules have identical behaviour and similar physicochemical and immunological properties when analysed by SDS electrophoresis, isoelectrofocusing and two-dimensional immunoelectrophoresis.Key words: T-kininogen, T-kinin, kallikrein–kininogen system.

Effects of salbutamol and Ro-20-1724 on airway and parenchymal mechanics in rats

1999 ◽  
Vol 87 (4) ◽  
pp. 1373-1380 ◽  
Author(s):  
Ferenc Peták ◽  
Janet L. Wale ◽  
Peter D. Sly
Keyword(s):  
Steady State ◽  
Airway Resistance ◽  
Brown Norway ◽  
Wave Tube ◽  
Norway Rat ◽  
Airway Caliber ◽  
Brown Norway Rat ◽  
Phosphodiesterase Type ◽  
Induced Changes ◽  
Norway Rats

We investigated the effects of a selective β2-agonist, salbutamol, and of phosphodiesterase type 4 inhibition with 4-(3-butoxy-4-methoxy benzyl)-2-imidazolidinone (Ro-20-1724) on the airway and parenchymal mechanics during steady-state constriction induced by MCh administered as an aerosol or intravenously (iv). The wave-tube technique was used to measure the lung input impedance (Zl) between 0.5 and 20 Hz in 31 anesthetized, paralyzed, open-chest adult Brown Norway rats. To separate the airway and parenchymal responses, a model containing an airway resistance (Raw) and inertance (Iaw), and a parenchymal damping (G) and elastance (H), was fitted to Zl spectra under control conditions, during steady-state constriction, and after either salbutamol or Ro-20-1724 delivery. In the Brown Norway rat, the response to iv MCh infusion was seen in Raw and G, whereas continuous aerosolized MCh challenge produced increases in G and H only. Both salbutamol, administered either as an aerosol or iv, and Ro-20-1724 significantly reversed the increases in Raw and G when MCh was administered iv. During the MCh aerosol challenge, Ro-20-1724 significantly reversed the increases in G and H, whereas salbutamol had no effect. These results suggest that, after MCh-induced changes in lung function, salbutamol increases the airway caliber. Ro-20-1724 is effective in reversing the airway narrowings, and it may also decrease the parenchymal constriction.

scholarly journals Intraspecific Differences Regarding Granular Polymorphism in Granular Ducts’ Cells in Rats’ Mandibular Gland

2018 ◽  
Vol 75 (1) ◽  
pp. 1
Author(s):  
Bianca MATOSZ ◽  
Flavia RUXANDA ◽  
Adrian Florin GAL ◽  
Vlad Emil LUCA ◽  
Viorel MICLĂUȘ
Keyword(s):  
Wistar Rats ◽  
Mandibular Gland ◽  
Wistar Rat ◽  
Excretory Duct ◽  
Brown Norway ◽  
Mandibular Glands ◽  
Norway Rat ◽  
Brown Norway Rat ◽  
Norway Rats ◽  
Granular Ducts

Mandibular gland ducts’ system in rodents consist of intralobular ducts (intercalated, granular, striated) and interlobular one (main excretory duct). Granular ducts are located between intercalated and striated ducts, being present only in mandibular gland of the mouse, rat, hamster and gerbil. The biological material used for this study was represented by two strains from the same species, three Wistar rats and three Brown Norway rats. After the animals were euthanized, the mandibular glands were harvested and then processed for histological investigations. The tissue fragments were sectioned at 5μm thickness and then stained the sections using Tricrom-Goldners method. Our results emphasize that the granular ducts are well developed; regarding the shape, they are convoluted in both Wistar and Brown Norway rats, without any significant differences between the two strains. In Wistar rat, the granules in granular ducts cells are small to medium in size, with discrete polymorphism. In Brown Norway rat, the cytoplasm is loaded with granules as in Wistar rat, but these are several times larger and more polymorphic.

scholarly journals Complete amino acid sequence of bovine colostrum low-M r cysteine proteinase inhibitor

FEBS Letters ◽  
1985 ◽  
Vol 186 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Masayuki Hirado ◽  
Susumu Tsunasawa ◽  
Fumio Sakiyama ◽  
Michio Niinobe ◽  
Setsuro Fujii
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Proteinase Inhibitor ◽  
Cysteine Proteinase ◽  
Bovine Colostrum ◽  
Cysteine Proteinase Inhibitor ◽  
Complete Amino Acid Sequence

Cloning of rat eotaxin: ozone inhalation increases mRNA and protein expression in lungs of Brown Norway rats

1998 ◽  
Vol 274 (1) ◽  
pp. L171-L176 ◽  
Author(s):  
Yukio Ishii ◽  
Manabu Shirato ◽  
Akihiro Nomura ◽  
Tohru Sakamoto ◽  
Yoshiyuki Uchida ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acute Inflammation ◽  
Brown Norway ◽  
Bronchial Epithelial ◽  
Inflammatory Conditions ◽  
Acid Protein ◽  
Mrna And Protein Expression ◽  
Pulmonary Infiltration ◽  
Norway Rats ◽  
Amino Acid Protein

The C-C chemokine eotaxin is thought to be important in the selective recruitment of eosinophils to the site of inflammation in guinea pigs, mice, and humans. We isolated the rat eotaxin gene to determine whether a similar molecule might play a role in the pulmonary infiltration of eosinophils during acute inflammation in the rat. The cDNA for rat eotaxin encoded a 97-amino acid protein containing a 74-amino acid mature eotaxin protein with 97.3% identity to mouse eotaxin. The recombinant protein encoded by this gene displayed specific chemotactic activity for eosinophils when analyzed with a microchemotactic chamber. The expression of eotaxin mRNA increased ∼1.6-fold immediately after exposure to ozone and was 4-fold higher after 20 h. The number of lavageable eosinophils at the same time points were 3- and 15-fold greater, respectively, than control eosinophils. Immunocytochemistry revealed that alveolar macrophages and bronchial epithelial cells were positive for eotaxin. These results suggest that eotaxin may be involved in the recruitment of eosinophils into the air spaces during certain inflammatory conditions in rats.

Brown Norway rats show impaired nNOS-mediated information transfer in renal autoregulationThis article is part of a Special Issue on Information Transfer in the Microcirculation.

2009 ◽  
Vol 87 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Xuemei Wang ◽  
William A. Cupples
Keyword(s):  
Information Transfer ◽  
Blood Pressure Reduction ◽  
Selective Inhibition ◽  
Macula Densa ◽  
Tubuloglomerular Feedback ◽  
Brown Norway ◽  
Norway Rat ◽  
Depressor Response ◽  
Norway Rats ◽  
Nnos Inhibition

Nonselective inhibition of NO synthase (NOS) augments myogenic autoregulation of renal blood flow (RBF) and profoundly reduces RBF. Previously in Wistar rats, we showed that augmented autoregulation, but not vasoconstriction, is duplicated by intrarenal inhibition of neuronal NOS (nNOS), whereas intrarenal inhibition of inducible NOS (iNOS) has no effect on RBF or on RBF dynamics. Thus macula densa nNOS transfers information from tubuloglomerular feedback to the afferent arteriole. This information flow requires that macula densa nNOS can sufficiently alter ambient NO concentration, that is, that endothelial NOS (eNOS) and iNOS do not alter local NO concentration. Because the Brown Norway rat often shows exaggerated responses to NOS inhibition and has peculiarities of renal autoregulation that are related to NO, we used this strain to study systemic and renal vascular responses to NOS inhibition. The first experiment showed transient blood pressure reduction by bolus i.v. acetylcholine that was dose-dependent in both strains and substantially prolonged in Brown Norway rats. The depressor response decayed more rapidly after nonselective NOS inhibition and the difference between strains was lost, indicating a greater activity of eNOS in Brown Norway rats. In Brown Norway rats, selective inhibition of iNOS reduced RBF (–16% ± 7%) and augmented myogenic autoregulation, whereas nNOS inhibition reduced RBF (–25% ± 4%) and did not augment myogenic autoregulation. The significant responses to intrarenal iNOS inhibition, the reduced modulation of autoregulation by nNOS inhibition, and the enhanced endothelial depressor response suggest that physiological signalling by NO within the kidney is impaired in Brown Norway rats because of irrelevant or inappropriate input of NO by eNOS and iNOS.

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