Immobilized wheat germ agglutinin separates small oligosaccharides derived from poly-N-acetyllactosaminoglycans of embryonal carcinoma cells

1988 ◽  
Vol 66 (5) ◽  
pp. 449-453 ◽  
Author(s):  
Ossi Renkonen ◽  
Pekka Mäkinen ◽  
Karl Hård ◽  
Jari Helin ◽  
Leena Penttilä
Keyword(s):  
Chromatographic Separation ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Binding Affinities ◽  
Embryonal Carcinoma Cells ◽  
Small Column ◽  
Teratocarcinoma Cells ◽  
Weak Binding

Five pure oligosaccharides derived from poly-N-acetyllactosaminoglycans of teratocarcinoma cells were chromatographed on immobilized wheat germ agglutinin (WGA). Three of them, Galβ1-4GlcNAc, GlcNAcβ1-3Gal, and GlcNAcβ1-3Galβ1-4GlcNAc, revealed only weak binding, but GlcNAcβ1-6Galβ1-4GlcNAc was bound moderately and GlcNAcβ1-6Gal was bound quite strongly. The differences in the binding affinities were large enough to allow chromatographic separation of the five oligosaccharides into three distinct fractions in a small column of WGA–agarose.

Wheat germ agglutinin chromatography of GlcNacβ1-3(GlcNAcβl-6)Gal and GlcNAcβ1-3(GlcNAcβ1-6)Galβ1-4GlcNAc, obtained by in vitro synthesis and by partial cleavage of teratocarcinoma poly-N-acetyllactosaminoglycans

1990 ◽  
Vol 68 (1) ◽  
pp. 44-53 ◽  
Author(s):  
Antti Seppo ◽  
Leena Penttilä ◽  
Anne Makkonen ◽  
Anne Leppänen ◽  
Ritva Niemelä ◽  
...  
Keyword(s):  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Embryonal Carcinoma ◽  
Periodate Oxidation ◽  
Carcinoma Cells ◽  
Teratocarcinoma Cell ◽  
Partial Acid Hydrolysis ◽  
In Vitro Synthesis ◽  
In Vitro Biosynthesis

GlcNAcβ1-3(GlcNAcβ1-6)[14C(U)]Gal and GlcNAcβ1-3(GlcNAcβ1-6)[14C(U)]Galβ1-4GlcNAc were prepared by in vitro synthesis. They were characterized by enzymatic sequencing, by partial acid hydrolysis, and by periodate oxidation experiments. The two saccharides were isolated also from partial acid hydrolysates of metabolically labeled poly-N- acetyllactosaminoglycans of murine embryonal carcinoma cells (line PC 13). The tetrasaccharide was retarded in a column of agarose-linked wheat germ agglutinin; the trisaccharide was strongly bound. Chromatography in this column separated the trisaccharide into two distinct peaks, which represented interconvertible molecules. Together with our previous data on linear teratocarcinoma saccharides, these findings show that affinity chromatography with immobilized wheat germ agglutinin can be advantageously used in fractionating radiolabeled oligo-N-acetyllactosaminoglycans and saccharides related to them.Key words: GlcNAcβ1-3(GlcNAcβ1-6)Gal, GlcNAcβ1-3(GlcNAcβ1-6)Galβ1-4GlcNAc, wheat germ agglutinin – agarose chromatography, in vitro biosynthesis, teratocarcinoma cell.

Neutral amino acid transport in embryonal carcinoma cells

1985 ◽  
Vol 122 (3) ◽  
pp. 379-386 ◽  
Author(s):  
John S. Zuzack ◽  
Richard J. Tasca ◽  
Stephen M. DiZio
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Neutral Amino Acid ◽  
Embryonal Carcinoma Cells ◽  
Acid Transport

Dickkopf-3 alters the morphological response to retinoic acid during neuronal differentiation of human embryonal carcinoma cells

2014 ◽  
Vol 74 (12) ◽  
pp. 1243-1254 ◽  
Author(s):  
Rocío Jiménez Alfonso ◽  
Irantzu Gorroño-Etxebarria ◽  
Miriam Rabano ◽  
Maria dM. Vivanco ◽  
Robert Kypta
Keyword(s):  
Retinoic Acid ◽  
Neuronal Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Morphological Response

scholarly journals Anticancer Activity of a Combination of Cisplatin and Fisetin in Embryonal Carcinoma Cells and Xenograft Tumors

2011 ◽  
Vol 10 (2) ◽  
pp. 255-268 ◽  
Author(s):  
Rakshamani Tripathi ◽  
Tanmoy Samadder ◽  
Sarika Gupta ◽  
Avadhesha Surolia ◽  
Chandrima Shaha
Keyword(s):  
Anticancer Activity ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells

Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1

2008 ◽  
Vol 90 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Yu Yang ◽  
Lanjing Zhang ◽  
Yanyu Wei ◽  
Hua Wang ◽  
Mariko Fukuma ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells
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