Rapid automated synthesis via diisopropyl phosphoramidite in situ activation. Chemical synthesis and cloning of a calmodulin gene

1986 ◽  
Vol 64 (6) ◽  
pp. 548-555 ◽  
Author(s):  
G. Alvarado-Urbina ◽  
R. Chiarello ◽  
D. Roberts ◽  
G. Vilain ◽  
F. Jurik ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Solid Support ◽  
Automated Synthesis ◽  
Dna Fragments ◽  
Cleavage Sites ◽  
Gene Coding ◽  
In Situ Activation ◽  
Restriction Endonuclease Cleavage

A gene coding for a calmodulin was synthesized and cloned. The chemical synthesis of the gene, coding for 149 amino acids, was achieved by the enzymatic ligation of 61 chemically synthesized DNA fragments. The DNA fragments were synthesized using a solid support with a diisopropyl phosphoramidite intermediate and in situ activation. The automated standard cycle time was 10 min/addition. The synthesizer was designed and constructed from inexpensive, readily available parts and controlled by a Commodore 64 computer. The gene possesses 27 unique, regularly spaced, restriction endonuclease cleavage sites to facilitate gene mutagenesis.

Erratum: Rapid automated synthesis via diisopropyl phosphoramidite in situ activation. Chemical synthesis and cloning of a calmodulin gene

1986 ◽  
Vol 64 (12) ◽  
pp. 1382-1382
Author(s):  
G. Alvarado-Urbina ◽  
R. Chiarello ◽  
D. Roberts ◽  
G. Vilain ◽  
F. Jurik ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Automated Synthesis ◽  
Calmodulin Gene ◽  
In Situ Activation

A Photoactivatable α5β1-Specific Integrin Ligand

2018 ◽  
Author(s):  
Roshna Vakkeel ◽  
Aleeza Farrukh ◽  
Aranzazu del Campo
Keyword(s):  
Cellular Response ◽  
Light Exposure ◽  
Matrix Composition ◽  
Cellular Behavior ◽  
Dynamic Variations ◽  
In Situ Activation ◽  
Controlled Exposure ◽  
Cellular Behaviour ◽  
Integrin Ligand

In order to study how dynamic changes of α5β1 integrin engagement affect cellular behaviour, photoactivatable derivatives of α5β1 specific ligands are presented in this article. The presence of the photoremovable protecting group (PRPG) introduced at a relevant position for integrin recognition, temporally inhibits ligand bioactivity. Light exposure at cell-compatible dose efficiently cleaves the PRPG and restores functionality. Selective cell response (attachment, spreading, migration) to the activated ligand on the surface is achieved upon controlled exposure. Spatial and temporal control of the cellular response is demonstrated, including the possibility to in situ activation. Photoactivatable integrin-selective ligands in model microenvironments will allow the study of cellular behavior in response to changes in the activation of individual integrins as consequence of dynamic variations of matrix composition.

Comparative study of MoS2 and Co/MoS2 catalysts prepared by ex situ/in situ activation of ammonium and tetraalkylammonium thiomolybdates

2004 ◽  
Vol 210 (1-2) ◽  
pp. 105-117 ◽  
Author(s):  
L. Alvarez ◽  
J. Espino ◽  
C. Ornelas ◽  
J.L. Rico ◽  
M.T. Cortez ◽  
...  
Keyword(s):  
Comparative Study ◽  
Ex Situ ◽  
In Situ Activation ◽  
Mos2 Catalysts

A facile one pot route for the synthesis of imide tethered peptidomimetics

2016 ◽  
Vol 14 (2) ◽  
pp. 556-563 ◽  
Author(s):  
Veladi Panduranga ◽  
Girish Prabhu ◽  
Roopesh Kumar ◽  
Basavaprabhu Basavaprabhu ◽  
Vommina V. Sureshbabu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Efficient Method ◽  
One Pot ◽  
Protected Amino Acid

A simple and efficient method for the synthesis of N,N’-orthogonally protected imide tethered peptidomimetics is presented. The imide peptidomimetics were synthesized by coupling the in situ generated selenocarboxylate of Nα-protected amino acids with Nα-protected amino acid azides in good yields.

In situ studies on alginic acid synthesis and other aspects of the metabolism of Laminaria digitata

1972 ◽  
Vol 50 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Johan A. Hellebust ◽  
Arne Haug
Keyword(s):  
Amino Acids ◽  
Time Course ◽  
Alginic Acid ◽  
Acid Synthesis ◽  
Short Term ◽  
Fresh Weight Basis ◽  
Mannuronic Acid ◽  
Guluronic Acid ◽  
Rate Of Photosynthesis

Amino acids, particularly alanine and aspartate, become more strongly labeled than mannitol in short-term 14C-photoassimilation experiments. The amino acids are the most likely sources of carbon for alginic acid synthesis and respiration in the dark, in contrast to mannitol, which appears to be relatively unavailable. Temperature is very important in determining the rate of loss of recent photoassimilate in L. digitata. The rate of photosynthesis, on a fresh weight basis, is much higher for blades than for stipes.The time course for incorporation of photoassimilated carbon into alginate differs for the stipe and blade both in light and dark periods. Very little 14C enters alginate in blades in the dark, while alginate in stipes acquires considerable amounts of activity during dark periods. Alginate in both blade and stipe acquires 14C predominantly in mannuronic acid residues of their alginate during short-term photoassimilation periods, while guluronic acid residues become relatively more rapidly labeled during dark periods.

Probing Structure Modification of Palladium Nanomaterials during Chemical Synthesis by using In Situ X-ray Diffraction: Electrochemical Properties

2015 ◽  
Vol 2 (4) ◽  
pp. 592-599 ◽  
Author(s):  
Yaovi Holade ◽  
Teko W. Napporn ◽  
Claudia Morais ◽  
Karine Servat ◽  
K. Boniface Kokoh
Keyword(s):  
Chemical Synthesis ◽  
Electrochemical Properties ◽  
Structure Modification ◽  
X Ray Diffraction ◽  
X Ray

Efficient Transamidation of Primary Carboxamides by in Situ Activation withN,N-Dialkylformamide Dimethyl Acetals

2006 ◽  
Vol 128 (50) ◽  
pp. 16406-16409 ◽  
Author(s):  
Thomas A. Dineen ◽  
Matthew A. Zajac ◽  
Andrew G. Myers
Keyword(s):  
In Situ Activation

Peptide Synthesis through Cell-Free Expression of Fusion Proteins Incorporating Modified Amino Acids as Latent Cleavage Sites for Peptide Release

ChemBioChem ◽  
2016 ◽  
Vol 17 (10) ◽  
pp. 908-912 ◽  
Author(s):  
Mantas Liutkus ◽  
Samuel A. Fraser ◽  
Karine Caron ◽  
Dannon J. Stigers ◽  
Christopher J. Easton
Keyword(s):  
Amino Acids ◽  
Peptide Synthesis ◽  
Fusion Proteins ◽  
Free Expression ◽  
Cleavage Sites ◽  
Peptide Release
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