The distribution of the stalk cell differentiation inducing factor and other lipids during the differentiation of Dictyostelium discoideum

1986 ◽  
Vol 64 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Nancy Neave ◽  
Linda Kwong ◽  
James I. S. MacDonald ◽  
Gerald Weeks
Keyword(s):  
Life Cycle ◽  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Polar Lipid ◽  
Steryl Ester ◽  
De Novo ◽  
Stalk Cell ◽  
Membrane Bound

The production of the stalk cell differentiation inducing factor (DIF) is restricted to the differentiation phase of the Dictyostelium discoideum life cycle. By the migrating pseudoplasmodial stage, the majority of the accumulated DIF is localized outside the pseudoplasmodia and the remaining pseudoplasmodial DIF is distributed between the intracellular and intercellular compartments. These results indicate possible reasons for the failure to detect gradients of DIF along the migrating pseudoplasmodia. De novo synthesized sterol is also localized predominantly outside the pseudoplasmodia. In contrast the majority of the de novo synthesized polar lipid and steryl ester are within the pseudoplasmodium. Of the de novo synthesized intracellular components, the polar lipid is almost entirely membrane bound and, although most of the sterol and steryl ester are also membrane bound, significant amounts are cytosolic. Intracellular DIF is also detectable in both membrane and cytosolic fractions.

Differanisole A, an inducer of the differentiation of Friend leukemic cells, induces stalk cell differentiation in Dictyostelium discoideum

FEBS Letters ◽  
1993 ◽  
Vol 322 (1) ◽  
pp. 73-75 ◽  
Author(s):  
Yuzuru Kubohara ◽  
Koji Okamoto ◽  
Yoshimasa Tanaka ◽  
Ken-ichi Asahi ◽  
Akira Sakurai ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Leukemic Cells ◽  
Stalk Cell

Ammonia depletion and DIF trigger stalk cell intact Dictyostelium discoideum slugs

Development ◽  
1989 ◽  
Vol 105 (3) ◽  
pp. 569-574 ◽  
Author(s):  
M. Wang ◽  
P. Schaap
Keyword(s):  
Cell Differentiation ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Stalk Cell

The differentiation-inducing factor, DIF, was induce stalk cell differentiation in Dictyostelium incubated as submerged monolayers. We investigated the regulates the differentiation of stalk cells in the was found that in migrating or submerged slugs DIF cell differentiation, which is most likely due to the antagonist. Cyclic AMP and ammonia were earlier antagonists in vitro. We show here that ammonia, but an antagonist for DIF-induced stalk cell can induce stalk cell differentiation when ammonia are enzymically depleted. However, depletion of cAMP increase the efficacy of DIF. We propose that the cell differentiation during early culmination may be drop in ammonia levels inside the organism.

Dictyopyrones, novel α-pyronoids isolated from Dictyostelium spp., promote stalk cell differentiation in Dictyostelium discoideum

2005 ◽  
Vol 73 (7) ◽  
pp. 377-384 ◽  
Author(s):  
Akiko Arai ◽  
Yukino Goto ◽  
Aiko Hasegawa ◽  
Kohei Hosaka ◽  
Haruhisa Kikuchi ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Stalk Cell

PARASEXUAL GENETIC ANALYSIS OF CELL PROPORTIONING MUTANTS OF DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1981 ◽  
Vol 99 (2) ◽  
pp. 183-196
Author(s):  
James H Morrissey ◽  
William F Loomis
Keyword(s):  
Cell Differentiation ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Double Mutant ◽  
Complementation Group ◽  
Stalk Cell ◽  
Recessive Mutation ◽  
Group Vi ◽  
Recessive Mutations

ABSTRACT Eight independently isolated mutants of Dictyostelium discoideum that differentiate exclusively into stalk cells make up one complementation group and carry single recessive mutations at the stalky locus, stkA, located on linkage group II. KY19, a previously described strain that differentiates into spores, but not stalk cells, was found to possess a recessive mutation defining the stalkless locus, stlA, located on linkage group VI. An analysis of the properties of these mutants, together with the phenotype of a haploid double mutant carrying stkA and stlA indicates that stlA results in poorly organized stalk tubes and incomplete stalk cell differentiation, while stkA causes all of the cells to differentiate into stalk cells, even when not enclosed in the stalk tube. The significance of these results is discussed in relation to current theories of pattern formation in D. discoideum.

Colchicine induces multiple axis formation and stalk cell differentiation in Dictyostelium discoideum

Development ◽  
1978 ◽  
Vol 47 (1) ◽  
pp. 195-206
Author(s):  
Danton H. O'Day ◽  
Antony J. Durston
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Immunofluorescent Staining ◽  
Stalk Cell ◽  
Axis Formation ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Long Time ◽  
Cellular Slime

Colchicine is shown to have several effects on the development of the pseudoplasmodia of the cellular slime mould Dictyostelium discoideum At concentrations of 0·01 M and above culmination was prevented, while differentiation of cells into stalk cells occurred at the rear of cell masses. Essentially all cells transformed into stalk cells when slugs were left on colchicine agar for a long time. At concentrations of 0·01 M normal slug architecture was maintained while above 0·025 M pseudoplasmodia reorganized into multiple mounds. Each of these mounds developed an apparently normal discrete tip which was devoid of prespore cells as shown by immunofluorescent staining. The same effects were observed in growing cultures and in regulating slugs treated with colchicine. The data are consistent with the ideas that microtubules are involved in the maintenance of slug architecture and in the differentiation of stalk cells. The modes by which these intracellular structures may operate in these functions are discussed.

Developmental regulation of a stalk cell differentiation-inducing factor in Dictyostelium discoideum

1982 ◽  
Vol 91 (1) ◽  
pp. 191-196 ◽  
Author(s):  
Jenny J. Brookman ◽  
Chris D. Town ◽  
Keith A. Jermyn ◽  
Robert R. Kay
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Developmental Regulation ◽  
Stalk Cell

scholarly journals Rates of degradation and synthesis of glycosidases de novo during growth and differentiation of Dictyostelium discoideum

1975 ◽  
Vol 148 (2) ◽  
pp. 169-177 ◽  
Author(s):  
D Every ◽  
J M Ashworth
Keyword(s):  
Amino Acids ◽  
Enzyme Activity ◽  
Life Cycle ◽  
Dictyostelium Discoideum ◽  
Growth Medium ◽  
Growth Phase ◽  
Enzyme Activities ◽  
De Novo ◽  
Antibody Preparation ◽  
Differentiated Cells

1. Injection of a purified preparation of β-N-acetylglucosaminidase from the spent growth medium of myxamoebae of Dictyostelium discoideum into rabbits gave rise to an antibody preparation containing both anti-α-glucosidase and anti-β-acetylglucosaminidase activities. 2. These two activities were shown to reside in different immunoglobulin molecules and it was concluded that the β-N-acetylglucosaminidase preparation contained trace amounts of highly antigenic α-glucosidase. 3. A single precipitin band having β-N-acetylglucosaminidase activity was formed in Ouchterlony plates when this antibody preparation was tested against extracts obtained from differentiated cells or from myxamoebae grown either axenically or on bacteria. 4. The antibody preparation was used to show that both β-N-acetylglucosaminidase and α-glucosidase molecules are synthesized de novo from isotopically labelled amino acids during both the growth and differentiation phases of the life cycle and to show that neither of these proteins is significantly degraded during the growth phase or during the first 9h of differentiation. 5. The rates of accumulation of these assayable enzyme activities are thus equal to their rates of synthesis during growth and early differentiation. 6. The factors regulating cellular enzyme activity during the life cycle of D. discoideum are discussed.

Induction by acid load of the maturation of prestalk cells in Dictyostelium discoideum

Development ◽  
1988 ◽  
Vol 104 (4) ◽  
pp. 669-681
Author(s):  
K. Inouye
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Cell Maturation ◽  
Stalk Cell ◽  
Body Construction ◽  
Acid Load ◽  
Cellular Slime ◽  
Plasma Membrane Proton Pump

During the process of fruiting body construction in the cellular slime mould Dictyostelium discoideum, prestalk cells become mature stalk cells in a well-controlled manner. To identify the natural inducer of stalk cell maturation, substances known to induce stalk cell differentiation under in vitro conditions, and some other related compounds, were examined for their effects in vivo on migrating slugs, the precursor structures of the fruiting bodies. Among these substances, addition of weak acids such as CO2, and addition followed by removal of weak bases such as NH3, strikingly induced the maturation of prestalk cells in situ in slugs. On the other hand, inhibitors of the plasma membrane proton pump did not efficiently induce the maturation of prestalk cells in intact slugs. Differentiation inducing factor (DIF), an endogenous inducer of prestalk differentiation, seemed to be an even poorer inducer of stalk cell maturation when applied to intact slugs. The activities of these substances in inducing stalk cell maturation showed a good correlation with their effects on the cytoplasmic pH (pHi) of prestalk cells; the larger the pHi drop, the stronger the induction of stalk cell maturation, suggesting a requirement for a pHi decrease for the maturation of prestalk cells. Based on these results, it was proposed that stalk cell differentiation, which is induced by DIF, is blocked halfway during normal development by (an) agent(s) that prevent(s) the decrease in pHi.

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