Effects of cytochalasin B on steroid-induced oocyte meiosis and centrifugally induced nuclear movement in the goldfish Carassius auratus

1985 ◽  
Vol 63 (7) ◽  
pp. 743-751 ◽  
Author(s):  
Hamid R. Habibi ◽  
Charles A. Lessman
Keyword(s):  
Centrifugal Force ◽  
Carassius Auratus ◽  
Cytochalasin B ◽  
Simple Technique ◽  
Germinal Vesicle ◽  
Oocyte Nucleus ◽  
Nuclear Movement ◽  
Goldfish Carassius Auratus ◽  
Oocyte Meiosis ◽  
High Doses

In goldfish, 17α, 20β-dihydroxyprogesterone (DHP) induced oocyte nucleus or germinal vesicle migration (GVM) and dissolution (GVD) in a dose-related fashion. Administration of cytochalasin B (CB) in the presence of DHP inhibited the steroid-induced GVM and GVD after 24 and 48 h of incubation. The presence of CB alone, at concentrations below 25 μg/mL, had no effect on GVM or GVD. Furthermore, CB, either alone or in combination with DHP, elicited significant increases in follicular diameter after 24 and 48 h of incubation. To test the effect of CB on ooplasmic viscoelasticity, fully grown follicles were centrifuged and the centrifugally induced germinal vesicle (GV) displacement was determined. Pretreatment (24 h) of follicles with high doses of CB (25 and 50 μg/mL) increased the movement of GV in a centripetal direction. However, at lower concentrations (0.005–5 μg/mL), CB treatment was without an effect on the centrifugally induced GV movement in the oocyte. The present study suggests involvement of microfilaments or other cytoskeletal components, sensitive to CB, in the mechanisms of GVM and GVD in goldfish oocytes. In addition, a simple technique has been described for testing ooplasmic viscoelasticity determined by movement of the GV under a centrifugal force.

Secretion of progestogens during induced ovulation in goldfish

1984 ◽  
Vol 62 (10) ◽  
pp. 1946-1949 ◽  
Author(s):  
R. E. Peter ◽  
M. Sokolowska ◽  
B. Truscott ◽  
J. Walsh ◽  
D. R. Idler
Keyword(s):  
Carassius Auratus ◽  
Germinal Vesicle ◽  
Serum Levels ◽  
Rapid Onset ◽  
Control Groups ◽  
Germinal Vesicle Breakdown ◽  
Luteinizing Hormone Releasing Hormone ◽  
Goldfish Carassius Auratus ◽  
Time Period ◽  
Short Time

Female goldfish (Carassius auratus L.) were held at 20 °C and ovulation was induced by injection of des-Gly10[D-Ala6] luteinizing hormone releasing hormone ethylamide (LHRH-A) plus pimozide, and 3 h later, LHRH-A. This treatment causes rapid onset of a prolonged surge of gonadotropin (GtH) release. At 6 h after the second set of injections, serum levels of GtH, 17α-hydroxyprogesterone (17α-P), and 17α-hydroxy-20β-dihydroprogesterone (17α,20β-P) are significantly higher than in control groups; oocytes have reached the germinal vesicle breakdown stage of maturation by this time. By 20 h after the second set of injections, seven of eight fish had ovulated in the LHRH-A and pimozide treatment group; the serum levels of GtH were greater than at 6 h, but serum concentrations of 17α-P and 17α,20β-P were decreased to control levels. The ovulated fish still retained ovulated oocytes. This indicates that goldfish have a preovulatory surge of secretion of progestogens, which may aid in induction of oocyte maturation and ovulation within a short time period, enabling the goldfish to synchronize ovulation with the photoperiod and to take advantage of favorable environmental conditions for spawning within a 24-h period.

Identification and molecular cloning of germinal vesicle lamin B3 in goldfish (Carassius auratus ) oocytes

2001 ◽  
Vol 268 (4) ◽  
pp. 932-939 ◽  
Author(s):  
Akihiko Yamaguchi ◽  
Masakane Yamashita ◽  
Michiyasu Yoshikuni ◽  
Yoshitaka Nagahama
Keyword(s):  
Molecular Cloning ◽  
Carassius Auratus ◽  
Germinal Vesicle ◽  
Goldfish Carassius Auratus

scholarly journals Dietary vitamin E on the reproductive performance of the fantail goldfish Carassius auratus auratus

2014 ◽  
Vol 12 (1) ◽  
pp. 14
Author(s):  
Harton Arfah ◽  
. Melati ◽  
Mia Setiawati
Keyword(s):  
Vitamin E ◽  
Reproductive Performance ◽  
Carassius Auratus ◽  
Gonadosomatic Index ◽  
Germinal Vesicle ◽  
Dietary Vitamin ◽  
Germinal Vesicle Breakdown ◽  
Goldfish Carassius Auratus ◽  
Egg Diameter

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph"> </p><p class="BasicParagraph">This research was conducted to examine the different dose of vitamin E in the diet on female broodstock reproduction performance of the fantail goldfish <em>Carassius auratus auratus</em>. This research consisted of four treatments with three replications. The use of vitamin E doses was 0, 125, 250, and 375 mg/kg. The vitamin E was dissolved in vegetable oil and mixed with albumen as a binder in feed. The vitamin E was sprayed at feed and was air dried. Female broodstock of the fantail goldfishes were reared for 40 days. The result showed that 375 mg/kg treatment performed the highest quality of reproduction. Egg diameter, gonadosomatic index, fecundity, and germinal vesicle breakdown of fish which are treated by 375 mg/kg vitamin E were respectively 0.92±0.05 mm, 8.86±4.62%, 56.00±29.18%, and 67.35±17.67% higher than control. Thus, 375 mg/kg of dietary vitamin E suplementating was a best dose to improve female broodstock productivity of the fantail goldfish</p><p class="BasicParagraph"> </p><p class="BasicParagraph">Keywords: female broodstock, fantail goldfish, vitamin E, reproduction quality</p><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"> </p><p class="BasicParagraph">Penelitian dilakukan untuk menguji suplementasi vitamin E dengan dosis berbeda dicampur ke dalam pakan komersial terhadap produktivitas induk betina ikan komet <em>Carassius auratus auratus</em>. Penelitian ini menggunakan empat perlakuan dengan tiga ulangan. Dosis vitamin E yang diberikan, yaitu 0, 125, 250, dan 375 mg/kg pakan. Vitamin E dilarutkan dalam minyak nabati dan dicampur dengan putih telur sebagai perekat pada pakan. Vitamin E disemprotkan ke pakan dan dikeringanginkan. Induk betina ikan komet pascasalin dengan bobot 72,78±19,47 g diberi perlakuan selama 40 hari. Hasil penelitian menunjukkan bahwa induk betina ikan komet yang diberi suplementasi vitamin E sebanyak 375 mg/kg dalam pakan memiliki diameter telur 0,92±0,05 mm, <em>gonadosomatic index</em> 8,86±4,62%, fekunditas 56,00±29,18 butir/g ikan, dan <em>germinal vesicle breakdown</em> 67,35±17,67% yang lebih tinggi dibandingkan kontrol. Dengan demikian, suplementasi vitamin E sebesar 375 mg/kg pada pakan adalah dosis terbaik dalam meningkatkan produktivitas induk betina ikan komet.</p><p class="BasicParagraph"> </p><p class="BasicParagraph">Kata kunci: induk betina, ikan komet, vitamin E, kualitas reproduksi</p><p> </p>

SEM of Hepatocytes and Biliary Passages in Goldfish Liver

1977 ◽  
Vol 35 ◽  
pp. 556-557
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Carassius Auratus ◽  
Cell Layer ◽  
Three Dimensional ◽  
Cell Types ◽  
Biliary System ◽  
Fish Food ◽  
Commercial Fish ◽  
Goldfish Carassius Auratus ◽  
Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

Induction of Gynogenesis and Androgenesis in Goldfish Carassius auratus (var. oranda)

2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas
Keyword(s):  
Carassius Auratus ◽  
Goldfish Carassius Auratus

Toxicity and deleterious impacts of selenium nanoparticles at supranutritional and imbalance levels on male goldfish (Carassius auratus) sperm

2021 ◽  
Vol 66 ◽  
pp. 126758
Author(s):  
Javad Seyedi ◽  
Mohammad Reza Kalbassi ◽  
Milad Esmaeilbeigi ◽  
Mohammad Behzadi Tayemeh ◽  
Jamshid Amiri Moghadam
Keyword(s):  
Carassius Auratus ◽  
Selenium Nanoparticles ◽  
Goldfish Carassius Auratus
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