Evaluation of the fatty acid selectivity of a phosphatidylinositol-specific cytosolic phospholipase C from pig and human platelets

1984 ◽  
Vol 62 (2-3) ◽  
pp. 115-120 ◽  
Author(s):  
Bruce J. Holub ◽  
Bernadette Celi
Keyword(s):  
Fatty Acid ◽  
Phospholipase C ◽  
Human Platelets ◽  
Gas Liquid Chromatography ◽  
Fatty Acid Selectivity ◽  
Sheep Liver ◽  
Cytosolic Phospholipase ◽  
Naturally Occurring ◽  
Relative Conversion ◽  
Fatty Acid Compositions

The fatty acid selectivity of cytosolic phospholipase C (phosphatidylinositol phosphodiesterase) from pig and human platelets towards phosphatidylinositol was evaluated. For this purpose, the relative conversion of rat liver phosphatidylinositol (enriched in stearate and arachidonate) and sheep liver phosphatidylinositol (enriched in stearate plus oleate and containing linoleate and arachidonate) was compared and, in addition, the fatty acid compositions of the diacylglycerol products were determined by gas–liquid chromatography. The cytosolic enzyme exhibited essentially complete specificity for phosphatidylinositol when choline-, ethanolamine-, serine-, or inositol-containing phospholipids labelled with [14C]stearate were tested as substrates. Similar percentage conversions of rat and sheep liver phosphatidylinositols to 1,2-diacylglycerol were found with phospholipase C from either pig or human platelets. Furthermore, the newly formed diacylglycerols and the unreacted phospholipid had fatty acid compositions which were very similar to the corresponding substrates. These results suggest that the phospholipase C from isolated platelet cytosol is highly selective towards phosphatidylinositol, but not with respect to the fatty acid composition of naturally occurring phosphatidylinositol. They also suggest that any preferential release of arachidonoyl diacylglycerol in stimulated human platelets is more likely controlled by compartmentation of the corresponding phosphatidylinositol precursor within platelet membranes and its availability, rather than directly by a marked enzyme preference for arachidonate-containing species.

Influence of cytoplasms on the fatty acid composition of two maize inbred lines

1976 ◽  
Vol 86 (1) ◽  
pp. 151-154 ◽  
Author(s):  
P. Gregory ◽  
C. O. Grogan
Keyword(s):  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Lines ◽  
Gas Liquid Chromatography ◽  
Maize Seeds ◽  
Maize Inbred Lines ◽  
Oleic And Linoleic Acids ◽  
Fatty Acid Compositions

SUMMARYGas liquid chromatography was used to investigate the influence of several T, S and unclassified cytoplasms on the fatty acid composition of oil from A632 and CrS4HLA maize seeds. The fatty acid compositions of the A632 and CrS4HLA seeds differed markedly from each other with respect to oleic and linoleic acids but were stable within each seed line with respect to all sixteen of the cytoplasms tested.

FATTY ACID COMPOSITION OF NORMAL MINK TISSUES

1966 ◽  
Vol 44 (2) ◽  
pp. 179-185 ◽  
Author(s):  
B. L. Walker ◽  
V. F. Lishchenko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Fatty Acids ◽  
Methyl Esters ◽  
Dietary Factors ◽  
Gas Liquid Chromatography ◽  
Healthy Female ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

Lipids, extracted from the adrenals, brain, erythrocytes, heart, kidney, liver, plasma, and spleen of normal healthy female mink, were transesterified with 1% sulphuric acid in methanol, and the resulting methyl esters were analyzed by gas–liquid chromatography after purification by thin-layer chromatography. All of the tissues examined contained higher concentrations of unsaturated than of saturated acids, the highest levels of unsaturated acids occurring in the lipids of heart, adrenals, and plasma, and of the essential fatty acids (ω6 series, with six carbon atoms after last double bond) in plasma, erythrocyte, and kidney lipids. The fatty acid compositions of mink tissues resemble those reported in the literature for the rat; detailed comparisons are not possible because of the known influence of dietary factors on tissue fatty acids.

Fatty-acid synthesis in the echinoderms: Asterias rubens, Echinus esculentus and Holothuria forskali

1968 ◽  
Vol 48 (2) ◽  
pp. 521-533 ◽  
Author(s):  
W. V. Allen
Keyword(s):  
Fatty Acid ◽  
Acid Synthesis ◽  
Gas Liquid Chromatography ◽  
Asterias Rubens ◽  
Phospholipid Classes ◽  
Tissue Lipids ◽  
Specific Activities ◽  
Branched Chain ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

The fatty-acid compositions of tissue lipids of Asterias rubens L., Echinus esculentus L. and Holothuria forskali Delia Chiaje were determined by means of gas-liquid chromatography. Considerable proportions of branched-chain, normal odd-numbered and eicosatetraenoic acids occur in the latter two animals. Tissues of all three animals were incubated with 1−14C-acetate. The relative specific activities of fatty-acid fractions isolated by thin-layer chromatography upon AgNO3-impregnated silica gel G fell in the order: saturates > monoenes — dienes > polyenes. The fatty-acid compositions of the alkoxydiglyceride, triglyceride and phospholipid classes of A. rubens hepatic caecal lipid were also determined.

FATTY ACID COMPOSITION AND GLYCERIDE STRUCTURE IN RATS FED RAPESEED OIL OR CORN OIL

1963 ◽  
Vol 41 (1) ◽  
pp. 43-49 ◽  
Author(s):  
B. M. Craig ◽  
C. G. Youngs ◽  
Joyce L. Beare ◽  
J. A. Campbell
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rapeseed Oil ◽  
Corn Oil ◽  
Abdominal Fat ◽  
The Body ◽  
Glyceride Structure ◽  
Gas Liquid Chromatography ◽  
Fat Depots ◽  
Fatty Acid Compositions

The fatty acid compositions of liver, skin, abdominal fat, and residual degutted carcass from each of five rats fed diets containing corn or rapeseed oil for 21 weeks were determined by gas-liquid chromatography. Statistical analysis of the results showed no significant variations among rats or among cutaneous, abdominal, and carcass fats. In these fat depots, the proportions of fatty acids resembled those of the diet except for palmitic, palmitoleic, oleic, and erucic acids. Glyceride compositions of the body fats were in agreement with those predicted by Vander Wal's distribution theory.

Principal Fatty Acids of Depot Fat and Milk Lipids from Harp Seal (Pagophilus groenlandica) and Hooded Seal (Cystophora cristata)

1968 ◽  
Vol 25 (11) ◽  
pp. 2419-2426 ◽  
Author(s):  
P. M. Jangaard ◽  
P. J. Ke
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Highly Unsaturated Fatty Acids ◽  
Hooded Seal ◽  
Cystophora Cristata ◽  
Milk Lipids ◽  
Iodine Values ◽  
Fatty Acid Compositions

The fatty acid compositions of six samples of depot fat taken from harp seals (Pagophilus groenlandica), ranging in age from 1 to 2 days to adult, and a sample of milk lipids, were determined by gas–liquid chromatography (GLC). Three depot fat samples taken from hooded seals (Cystophora cristata), a sample of milk lipids from this species, and commercial seal oils from 4 years' production were similarly analyzed.No significant differences could be found between the composition of the milk lipids and the depot fat of the harp seals at the different ages, other than slightly lower iodine values in the younger animals (avg 140.6 vs. 148.9). The lipid samples from hooded seals had low iodine values (107–127) and correspondingly lower concentration of highly unsaturated fatty acids. The ratio of the fatty acids 22:5ω3/22:6ω3 could be used to differentiate between depot fat from harp seals (ratio 0.51–0.74) and that from hooded seals (ratio 0.25–0.42) in the samples analyzed.Commercial oils, which consist essentially of oil from harp seals, were similar in composition to oil prepared from this species alone.No difference could be found in the iodine values or fatty acid compositions of samples taken from various parts of the animal or from samples representing a cross section of blubber.

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