Nuclease sensitivity of postreplicated chromatin from Ehrlich ascites tumour cells

1981 ◽  
Vol 59 (1) ◽  
pp. 22-29
Author(s):  
Brian L. A. Miki ◽  
John J. Heikkila ◽  
Ian R. Brown
Keyword(s):  
Structural Modification ◽  
Micrococcal Nuclease ◽  
Base Pairs ◽  
Selective Labelling ◽  
Dna Repeat ◽  
Ehrlich Ascites ◽  
Nuclease Digestion ◽  
Ehrlich Ascites Tumour ◽  
Ascites Tumour Cells ◽  
Ehrlich Ascites Tumour Cells

Chromatin appears to undergo structural modification after replication and before integration into bulk chromatin. In ascites cells, postreplicated chromatin displays a transient resistance to digestion with micrococcal nuclease. This resistance may be correlated with a shorter DNA repeat length (178 base pairs) than that found in bulk chromatin (187 base pairs). Selective labelling or selective digestion of DNA sequence classes could not account for these observations. In both bulk and postreplicated chromatin, three electrophoretic types of mononucleosomes were found. Postreplicated mononucleosome types showed selective sensitivities to nuclease digestion whereas bulk mononucleosome types did not.

Characterization of mononucleosomes and associated glycoproteins from Ehrlich ascites tumour cells

1980 ◽  
Vol 58 (11) ◽  
pp. 1261-1269 ◽  
Author(s):  
Brian L. A. Miki ◽  
James W. Gurd ◽  
Ian R. Brown
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Apparent Molecular Weight ◽  
Micrococcal Nuclease ◽  
Nonhistone Proteins ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Tumour ◽  
Ascites Tumour Cells ◽  
Ehrlich Ascites Tumour Cells

Mononucleosomes generated by the digestion of ascites nuclei with micrococcal nuclease were resolved into three types by polyacrylamide gel electrophoresis. All three types were present in early and late digests and a precursor–product relationship was not apparent. Each mononucleosome type was associated with a unique pattern of subnucleosome DNA fragments and differences were apparent in histone and nonhistone proteins. On the basis of reactivity to 125I-labelled concanavalin A and labelling with [3H]glucosamine, glycoproteins were identified as components of two of the mononucleosome types. The principal glycoprotein species associated with mononucleosomes had an apparent molecular weight of 130 000.

scholarly journals The transport and oxidation of succinate by Ehrlich ascites-tumour cells

1976 ◽  
Vol 160 (1) ◽  
pp. 121-123 ◽  
Author(s):  
T L Spencer
Keyword(s):  
Ph Dependence ◽  
Organic Anion ◽  
Tumour Cells ◽  
Cell Integrity ◽  
Carrier System ◽  
Ascites Tumour ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Tumour ◽  
Ascites Tumour Cells ◽  
Ehrlich Ascites Tumour Cells

The transport and oxidation of succinate by functionally intact Ehrlich ascites-tumour cells was investigated. On the basis of pH dependence and inhibitor sensitivity it was concluded that succinate may be transported across the cell membrane by the organic anion carrier system. Thus the ability of isolated Ehrlich cells to oxidize succinate is real, and is not necessarily a result of damage to cell integrity.

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