Yeast, rye, and calf histones. Similarities and differences detected by electrophoretic and immunological methods

1980 ◽  
Vol 58 (5) ◽  
pp. 405-409 ◽  
Author(s):  
Anne Tessier ◽  
Birgitte Roland ◽  
Claude Gauthier ◽  
William A. Anderson ◽  
Dominick Pallotta
Keyword(s):  
Protein Component ◽  
The Other ◽  
Immunological Methods ◽  
Major Protein ◽  
Histone H4 ◽  
Exclusion Chromatography ◽  
Core Histones ◽  
Similarities And Differences ◽  
Differential Solubility ◽  
Immunological Distance

Yeast histones H2A, H2B, and H3 were purified using the standard histone purification procedures of differential solubility and exclusion chromatography. Yeast histone H4 was isolated by the same methods in a fraction containing one other major protein component. The four yeast core histones were identified by their reactions with antisera against rye and (or) calf histone fractions as well as by their electrophoretic, chromatographic, and solubility properties. The immunological distances between yeast H2B and rye and calf H2B fractions are substantial, as is the rye–calf distance for H2B. The immunological distance between yeast H2A and rye H2A is also large and is similar to the rye H2A – calf H2A distance. On the other hand, the immunological distance between yeast H3 and rye and calf H3 is much greater than that between rye H3 and calf H3. These and other results indicate that yeast H3 differs appreciably from the H3 of higher eucaryotes.

Orosensory Perception, Speech Production, and Deafness

1973 ◽  
Vol 16 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Milo E. Bishop ◽  
Robert L. Ringel ◽  
Arthur S. House
Keyword(s):  
High School ◽  
Speech Production ◽  
Poor Performance ◽  
Normal Hearing ◽  
The Other ◽  
Functional Disorders ◽  
Form Discrimination ◽  
School Subjects ◽  
Oral Form ◽  
Similarities And Differences

The oral form-discrimination abilities of 18 orally educated and oriented deaf high school subjects were determined and compared to those of manually educated and oriented deaf subjects and normal-hearing subjects. The similarities and differences among the responses of the three groups were discussed and then compared to responses elicited from subjects with functional disorders of articulation. In general, the discrimination scores separated the manual deaf from the other two groups, particularly when differences in form shapes were involved in the test. The implications of the results for theories relating orosensory-discrimination abilities are discussed. It is postulated that, while a failure in oroperceptual functioning may lead to disorders of articulation, a failure to use the oral mechanism for speech activities, even in persons with normal orosensory capabilities, may result in poor performance on oroperceptual tasks.

The Kidney and Fibrinolysis

1970 ◽  
Vol 24 (01/02) ◽  
pp. 026-032 ◽  
Author(s):  
N. A Marsh
Keyword(s):  
Molecular Weight ◽  
Plasminogen Activator ◽  
Enzyme System ◽  
Normal Animal ◽  
Fibrinolytic Enzyme ◽  
The Other ◽  
Exclusion Chromatography ◽  
Normal Urine ◽  
Renal Origin ◽  
Molecular Exclusion Chromatography

SummaryMolecular exclusion chromatography was performed on samples of urine from normal and aminonucleoside nephrotic rats. Normal urine contained 2 peaks of urokinase activity, one having a molecular weight of 22,000 and the other around 200,000. Nephrotic urine contained three peaks of activity with MW’s 126,000, 60,000 and 30,000. Plasma activator determined from euglobulin precipitate had a MW. in excess of 200,000. The results indicate that in the normal animal, plasma plasminogen activator does not escape into the urine in substantial quantities but under the conditions of extreme proteinuria there may be some loss through the kidney. The alteration in urokinase output in nephrotic animals indicates a greatly disordered renal fibrinolytic enzyme system.The findings of this study largely support the hypothesis that plasma plasminogen activator of renal origin and urinary plasminogen activator (urokinase) are different molecular species.

Tourists’ and Residents’ Impressions of A Heritage Tourism Site: The Case of Kampong Taman Sari, Indonesia

2014 ◽  
Vol 8 (3) ◽  
pp. 181 ◽  
Author(s):  
Jenny Ernawati ◽  
Gary T. Moore
Keyword(s):  
Heritage Tourism ◽  
Semantic Differential ◽  
The Other ◽  
Measuring Instrument ◽  
Built Heritage ◽  
Other Hand ◽  
Similarities And Differences

The interface between tourism and built heritage is complicated because much built heritage is located in the middle of living communities. Questions arise about how to achieve a balance between the expectations of tourists and the community. To study this question, this paper reports on tourists’ and residents’ impressions of an international heritage tourism site, the Kampong Taman Sari in Indonesia. Using a linear-numeric semantic differential as the measuring instrument and nine consensus photographs of the site as stimuli, the study investigated similarities and differences in impressions between three groups: tourists (international and domestic) and residents. Three principal dimensions were found to underlie impressions of the site: Attractiveness, Organisation, and Novelty. Significant differences were found among all three groups in their impressions of Attractiveness. In terms of impressions of the Organisation of the site, international and domestic tourists have similar impressions but these differ significantly from the impressions of residents. On the other hand, domestic tourists and residents have similar impressions of the Novelty of the site, which is evaluated differently by international tourists.

scholarly journals Identification and Characterization of the Genes Encoding the Core Histones and Histone Variants of Neurospora crassa

Genetics ◽  
2002 ◽  
Vol 160 (3) ◽  
pp. 961-973 ◽  
Author(s):  
Shan M Hays ◽  
Johanna Swanson ◽  
Eric U Selker
Keyword(s):  
Neurospora Crassa ◽  
Histone Variants ◽  
Null Alleles ◽  
Histone Genes ◽  
Histone H4 ◽  
Coding Regions ◽  
The Core ◽  
Genomic Arrangement ◽  
Genes Encoding ◽  
Core Histones

Abstract We have identified and characterized the complete complement of genes encoding the core histones of Neurospora crassa. In addition to the previously identified pair of genes that encode histones H3 and H4 (hH3 and hH4-1), we identified a second histone H4 gene (hH4-2), a divergently transcribed pair of genes that encode H2A and H2B (hH2A and hH2B), a homolog of the F/Z family of H2A variants (hH2Az), a homolog of the H3 variant CSE4 from Saccharomyces cerevisiae (hH3v), and a highly diverged H4 variant (hH4v) not described in other species. The hH4-1 and hH4-2 genes, which are 96% identical in their coding regions and encode identical proteins, were inactivated independently. Strains with inactivating mutations in either gene were phenotypically wild type, in terms of growth rates and fertility, but the double mutants were inviable. As expected, we were unable to isolate null alleles of hH2A, hH2B, or hH3. The genomic arrangement of the histone and histone variant genes was determined. hH2Az and the hH3-hH4-1 gene pair are on LG IIR, with hH2Az centromere-proximal to hH3-hH4-1 and hH3 centromere-proximal to hH4-1. hH3v and hH4-2 are on LG IIIR with hH3v centromere-proximal to hH4-2. hH4v is on LG IVR and the hH2A-hH2B pair is located immediately right of the LG VII centromere, with hH2A centromere-proximal to hH2B. Except for the centromere-distal gene in the pairs, all of the histone genes are transcribed toward the centromere. Phylogenetic analysis of the N. crassa histone genes places them in the Euascomycota lineage. In contrast to the general case in eukaryotes, histone genes in euascomycetes are few in number and contain introns. This may be a reflection of the evolution of the RIP (repeat-induced point mutation) and MIP (methylation induced premeiotically) processes that detect sizable duplications and silence associated genes.

Isolation of cowpox virus a-type inclusions and characterization of their major protein component

Virology ◽  
1986 ◽  
Vol 149 (2) ◽  
pp. 174-189 ◽  
Author(s):  
Dhavalkumar D. Patel ◽  
David J. Pickup ◽  
Wolfgang K. Joklik
Keyword(s):  
Protein Component ◽  
Major Protein ◽  
Cowpox Virus

scholarly journals The immunological characterization of several human ribonucleases by using primary binding tests

1977 ◽  
Vol 163 (3) ◽  
pp. 419-426 ◽  
Author(s):  
E A Neuwelt ◽  
M Schmukler ◽  
M S Niziak ◽  
P B Jewett ◽  
C C Levy
Keyword(s):  
Human Plasma ◽  
Cross Reaction ◽  
The Other ◽  
Human Pancreas ◽  
Human Tissues ◽  
Antigenic Difference ◽  
Immunological Characterization ◽  
Similarities And Differences

RNAases (ribonucleases), purified from four human tissues, as well as bovine pancreatic RNAase (RNAase A), were studied by immunodiffusion methods and by two different primary binding tests. The enzymes fell into two groups immunologically, those purified from plasma and pancreas in one and those from spleen and liver in the other. No antigenic cross-reaction between the two groups was detected by any of the immunoassays used. There was a slight antigenic cross-reaction between the human and bovine pancreatic RNAases. The liver and spleen RNAases were immunologically identical by all criteria used, whereas a small but consistent antigenic difference between the human plasma and human pancreas enzymes was detected. The significance of this difference between the human plasma and pancreas RNAases is discussed in relation to similarities and differences in their properties.

scholarly journals THE ABSORPTION OF PROTEIN SOLUTIONS FROM THE PULMONARY ALVEOLI

1937 ◽  
Vol 66 (4) ◽  
pp. 449-458 ◽  
Author(s):  
Cecil K. Drinker ◽  
Madeleine Field Warren ◽  
Margaret MacLanahan
Keyword(s):  
Thoracic Duct ◽  
Horse Serum ◽  
Molecular Size ◽  
The Other ◽  
Immunological Methods ◽  
Protein Solutions ◽  
Blood Capillaries ◽  
Egg Albumin ◽  
Pulmonary Alveoli ◽  
The Right

Horse serum, crystallized hemoglobin, and crystallized egg albumin have been injected into the lung alveoli of dogs in which the entrances of the right lymphatics have been tied and the thoracic duct cannulated. Samples of blood and lymph have been taken following this injection. Only after several hours in the case of the horse serum and hemoglobin have these proteins been detected by immunological methods and invariably they have appeared first in the blood. Egg albumin also enters the blood capillaries, but much more rapidly than the other two proteins, due probably to the smaller molecular size.

scholarly journals In Trypanosoma brucei RNA Editing, TbMP18 (Band VII) Is Critical for Editosome Integrity and for both Insertional and Deletional Cleavages

2006 ◽  
Vol 27 (2) ◽  
pp. 777-787 ◽  
Author(s):  
Julie A. Law ◽  
Sean O'Hearn ◽  
Barbara Sollner-Webb
Keyword(s):  
Rna Interference ◽  
Trypanosoma Brucei ◽  
Rna Editing ◽  
Protein Complex ◽  
Substrate Recognition ◽  
The Other ◽  
Major Protein

ABSTRACT In trypanosome RNA editing, uridylate (U) residues are inserted and deleted at numerous sites within mitochondrial pre-mRNAs by an ∼20S protein complex that catalyzes cycles of cleavage, U addition/U removal, and ligation. We used RNA interference to deplete TbMP18 (band VII), the last unexamined major protein of our purified editing complex, showing it is essential. TbMP18 is critical for the U-deletional and U-insertional cleavages and for integrity of the ∼20S editing complex, whose other major components, TbMP99, TbMP81, TbMP63, TbMP52, TbMP48, TbMP42 (bands I through VI), and TbMP57, instead sediment as ∼10S associations. Additionally, TbMP18 augments editing substrate recognition by the TbMP57 terminal U transferase, possibly aiding the recognition component, TbMP81. The other editing activities and their coordination in precleaved editing remain active in the absence of TbMP18. These data are reminiscent of the data on editing subcomplexes reported by A. Schnaufer et al. (Mol. Cell 12:307-319, 2003) and suggest that these subcomplexes are held together in the ∼20S complex by TbMP18, as was proposed previously. Our data additionally imply that the proteins are less long-lived in these subcomplexes than they are when held in the complete editing complex. The editing endonucleolytic cleavages being lost when the editing complex becomes fragmented, as upon TbMP18 depletion, should be advantageous to the trypanosome, minimizing broken mRNAs.

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