N-Acylation of tyramines: purification and characterization of an arylamine N-acetyltransferase from rat brain and liver

1979 ◽  
Vol 57 (10) ◽  
pp. 1204-1209 ◽  
Author(s):  
Peter H. Yu ◽  
Alan A. Boulton
Keyword(s):  
Monoamine Oxidase ◽  
Rat Brain ◽  
Kinetic Parameters ◽  
Biogenic Amines ◽  
Significant Activity ◽  
Purification And Characterization ◽  
Rat Brain And Liver ◽  
The Brain ◽  
Optimal Ph

The N-acylation of tyramine isomers and other biogenic amines has been studied. The liver exhibits the highest activity towards tyramines, while the brain exhibits a low but significant activity. In the brain, tyramine N-acylation activity was heterogenously distributed. The arylamine N-acetyltransferase has been partially purified from both rat liver and brain, the two enzymes being quite similar with respect to their chromatographic properties, optimal pH requirement (pH 7.8), and their kinetic parameters. The product N-acetyltyramine is not oxidized by liver amidohydrolase or monoamine oxidase.

Partial purification and characterization of rat brain myo-inositol-1-phosphatase

1987 ◽  
Vol 15 (3) ◽  
pp. 429-430 ◽  
Author(s):  
F. CAREY ◽  
J. A. CREBA ◽  
P. J. FRENCH ◽  
A. D. SHORT ◽  
M. WALTERS ◽  
...  
Keyword(s):  
Rat Brain ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Inositol 1

Partial purification and characterization of two phosvitin phosphatases from rat brain

1996 ◽  
Vol 28 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Taxiarchis P. Geladopoulos ◽  
Theodore G. Sotiroudis ◽  
Athanasios E. Evangelopoulos
Keyword(s):  
Rat Brain ◽  
Partial Purification ◽  
Purification And Characterization

Characterization of two ‘mitochondrial’ particulates from rat brain

1960 ◽  
Vol 198 (2) ◽  
pp. 467-470 ◽  
Author(s):  
Dennis R. Dahl ◽  
Roberta J. Jacobs ◽  
Frederick E. Samson
Keyword(s):  
Rat Brain ◽  
Tricarboxylic Acid Cycle ◽  
Chemical Properties ◽  
Oxidative Capacity ◽  
Brain Mitochondria ◽  
P Fraction ◽  
Tricarboxylic Acid Cycle Intermediates ◽  
Physical And Chemical ◽  
The Brain

Two particulate fractions were isolated from a preparation of rat brain mitochondria and some of the physical and chemical properties were studied. The distinguishing physical difference was the color; the heavier, more densely packed particles (P) were dark, while the lighter, loosely packed particles (W) were almost white. The amount of (P) and (W) are approximately equal. P has a higher concentration of protein than W but the nucleic acid (RNA and DNA) concentration is about the same. The per cent water of W is slightly greater than P. The P fraction was capable of oxidative-phosphorylation with several tricarboxylic acid cycle intermediates but W had no oxidative capacity. P appears to undergo the typical ‘swelling’ of mitochondria whereas W does not. P and W both increase in amount with the neonatal maturation of the brain.

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