Inhibition of cell-free protein synthesis by analogues of aurintricarboxylic acid

1978 ◽  
Vol 56 (12) ◽  
pp. 1162-1167 ◽  
Author(s):  
B. L. Kreamer ◽  
J. Anderson ◽  
D. S. H. Liu ◽  
M. B. Sparks ◽  
A. Richardson
Keyword(s):  
Protein Synthesis ◽  
Wheat Germ ◽  
Potent Inhibitor ◽  
Ring System ◽  
Chick Brain ◽  
Free Protein ◽  
Aurintricarboxylic Acid ◽  
Carboxylic Acid Groups ◽  
Post Mitochondrial Supernatant ◽  
Cell Free Protein Synthesis

Aurintricarboxylic acid is a potent inhibitor of cell-free protein synthesis by the post-mitochondrial supernatant of chick brain and the translation of mRNA by wheat germ lysate. Comparison of commercially available and chemically synthesized analogues of aurintricarboxylic acid indicates that the unique aurin triphenyl methane ring system and the carboxylic acid groups are both necessary for inhibition of cell-free protein synthesis in both systems.

Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli, wheat germ, and rabbit reticulocytes

2008 ◽  
Vol 133 (2) ◽  
pp. 183-189 ◽  
Author(s):  
Mami Hino ◽  
Masatoshi Kataoka ◽  
Kazuaki Kajimoto ◽  
Takenori Yamamoto ◽  
Jun-Ichi Kido ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Wheat Germ ◽  
Cell Extract ◽  
Crude Cell Extract ◽  
Free Protein ◽  
Cell Free Protein Synthesis ◽  
Rabbit Reticulocytes

scholarly journals De novo reconstitution of chromatin using wheat germ cell‐free protein synthesis

FEBS Open Bio ◽  
2021 ◽  
Author(s):  
Yaeta Endo ◽  
Nobuaki Takemori ◽  
Szilvia K. Nagy ◽  
Kei‐ichi Okimune ◽  
Rohinton Kamakaka ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Germ Cell ◽  
Wheat Germ ◽  
De Novo ◽  
Free Protein ◽  
Cell Free Protein Synthesis

Stabilization effect of zeolite on DHFR mRNA in a wheat germ cell-free protein synthesis system

2000 ◽  
Vol 89 (2) ◽  
pp. 193-195 ◽  
Author(s):  
Gyoo Yeol Jung ◽  
Eun Yeol Lee ◽  
You-Eil Kim ◽  
Byeong Woo Jung ◽  
Sang Hyeon Kang ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Germ Cell ◽  
Wheat Germ ◽  
Synthesis System ◽  
Free Protein ◽  
Stabilization Effect ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

Genome-scale, biochemical annotation method based on the wheat germ cell-free protein synthesis system

2004 ◽  
Vol 65 (11) ◽  
pp. 1549-1555 ◽  
Author(s):  
Tatsuya Sawasaki ◽  
Yoshinori Hasegawa ◽  
Ryo Morishita ◽  
Motoaki Seki ◽  
Kazuo Shinozaki ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Germ Cell ◽  
Wheat Germ ◽  
Synthesis System ◽  
Free Protein ◽  
Annotation Method ◽  
Genome Scale ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

scholarly journals Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology

2021 ◽  
Vol 8 ◽  
Author(s):  
Marie-Laure Fogeron ◽  
Lauriane Lecoq ◽  
Laura Cole ◽  
Matthias Harbers ◽  
Anja Böckmann
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Germ Cell ◽  
Structural Biology ◽  
Wheat Germ ◽  
New Technologies ◽  
Basic Research ◽  
Free Protein ◽  
Eukaryotic Proteins ◽  
Cell Free Protein Synthesis

Cell-free protein synthesis (CFPS) systems are gaining more importance as universal tools for basic research, applied sciences, and product development with new technologies emerging for their application. Huge progress was made in the field of synthetic biology using CFPS to develop new proteins for technical applications and therapy. Out of the available CFPS systems, wheat germ cell-free protein synthesis (WG-CFPS) merges the highest yields with the use of a eukaryotic ribosome, making it an excellent approach for the synthesis of complex eukaryotic proteins including, for example, protein complexes and membrane proteins. Separating the translation reaction from other cellular processes, CFPS offers a flexible means to adapt translation reactions to protein needs. There is a large demand for such potent, easy-to-use, rapid protein expression systems, which are optimally serving protein requirements to drive biochemical and structural biology research. We summarize here a general workflow for a wheat germ system providing examples from the literature, as well as applications used for our own studies in structural biology. With this review, we want to highlight the tremendous potential of the rapidly evolving and highly versatile CFPS systems, making them more widely used as common tools to recombinantly prepare particularly challenging recombinant eukaryotic proteins.

scholarly journals Cell-free Protein Synthesis by Wheat Germ Extracts.

2003 ◽  
Vol 45 (1) ◽  
pp. 3-8
Author(s):  
Yuzuru TOZAWA ◽  
Tatsuya SAWASAKI ◽  
Yaeta ENDO
Keyword(s):  
Protein Synthesis ◽  
Wheat Germ ◽  
Free Protein ◽  
Cell Free Protein Synthesis ◽  
Wheat Germ Extracts
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