Heterogeneity of human plasma low density lipoprotein

1978 ◽  
Vol 56 (10) ◽  
pp. 977-980 ◽  
Author(s):  
Bernard Rubenstein
Keyword(s):  
Human Plasma ◽  
Column Chromatography ◽  
Salt Concentration ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Salt ◽  
High Salt Concentration ◽  
Low Density ◽  
Nacl Concentration

Low density lipoprotein (LDL) was fractionated into subspecies by the use of DEAE-agarose column chromatography and the peptide compositions of the LDL subspecies which eluted at different NaCl concentrations were determined. LDL which elutes at low NaCl concentration has relatively less non-B apoprotein than does LDL which elutes at high salt concentration. The LDL subspecies which elute at high NaCl concentration contain more apo A-1 than do those which elute at the lower NaCl molarity. These results indicate that LDL consists of subfractions which differ in their peptide compositions.

Fractionation of human low density lipoprotein by column chromatography

1976 ◽  
Vol 54 (12) ◽  
pp. 1023-1028 ◽  
Author(s):  
Bernard Rubenstein ◽  
George Steiner
Keyword(s):  
Column Chromatography ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cholesterol Content ◽  
Low Density ◽  
Elution Volume ◽  
Protein Ratio ◽  
Nacl Concentration ◽  
Ldl Subfractions

We have devised a method to fractionate low density lipoprotein (LDL) into subspecies by means of column chromatography. DEAE-agarose columns, 2.6 × 60 cm, were loaded with LDL (25–45 mg LDL protein) and eluted with a 0.045–0.13 M NaCl gradient. The LDL eluted over a volume of 900 ml. Specific portions of the eluted LDL, reapplied to a column identical with the original, reelute at about the same point. Altering the NaCl concentration of the elution fluid changed the elution volume. The cholesterol-protein ratio of the LDL subfractions was progressively lower in fractions eluting at higher NaCl concentrations. These results indicate the LDL is not a homogenous lipoprotein species but consists of subfractions which differ in at least charge and cholesterol content.

Studies on the Thromboplastic Effect of Human Plasma Lipoproteins

1976 ◽  
Vol 35 (01) ◽  
pp. 178-185 ◽  
Author(s):  
Helena Sandberg ◽  
Lars-Olov Andersson
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Platelet Rich Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Free Plasma ◽  
Good Agreement

SummaryHuman plasma lipoprotein fractions were prepared by flotation in the ultracentrifuge. Addition of these fractions to platelet-rich, platelet-poor and platelet-free plasma affected the partial thromboplastin and Stypven clotting times to various degrees. Addition of high density lipoprotein (HDL) to platelet-poor and platelet-free plasma shortened both the partial thromboplastin and the Stypven time, whereas addition of low density lipoprotein and very low density lipoprotein (LDL + VLDL) fractions only shortened the Stypven time. The additions had little or no effect in platelet-rich plasma.Experiments involving the addition of anti-HDL antibodies to plasmas with different platelet contents and measuring of clotting times produced results that were in good agreement with those noted when lipoprotein was added. The relation between structure and the clot-promoting activity of various phospholipid components is discussed.

Modifications of low density lipoprotein induced by the interaction with human plasma glycosaminoglycan-protein complexes

1994 ◽  
Vol 1212 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Gian Mario Cherchi ◽  
Marilena Formate ◽  
Pierina Demuro ◽  
Massimo Masserini ◽  
Ida Varani ◽  
...  
Keyword(s):  
Human Plasma ◽  
Protein Complexes ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density

Low-density lipoprotein modification occurring in human plasma

1998 ◽  
Vol 138 (1) ◽  
pp. 183-195 ◽  
Author(s):  
V.V Tertov ◽  
V.V Kaplun ◽  
I.A Sobenin ◽  
A.N Orekhov
Keyword(s):  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density

3.P.140 Multiple modification of low density lipoprotein occurs in human plasma

1997 ◽  
Vol 134 (1-2) ◽  
pp. 227-228
Author(s):  
V. Tertov ◽  
V. Kaplun ◽  
I. Sobenin ◽  
A. Orekhov
Keyword(s):  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density
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