Levels of coenzyme A – glutathione mixed disulfide in Escherichia coli

1978 ◽  
Vol 56 (7) ◽  
pp. 753-759 ◽  
Author(s):  
Peter C. Loewen
Keyword(s):  
Escherichia Coli ◽  
Coenzyme A ◽  
Anaerobic Fermentation ◽  
Carbon Sources ◽  
Lower Proportion ◽  
Fermentable Sugars ◽  
Glucose Starvation ◽  
Aerobic Growth ◽  
Mixed Disulfide ◽  
Oxygen Starvation

The pool of coenzyme A – glutathione mixed disulfide (CoASSG) rapidly increased 2.0 times in response to oxygen starvation and 1.5 times in response to glucose starvation but did not change following ammonia starvation. The increase in the CoASSG pool resulted from an increase in the CoASSG fraction of the CoA pool from 42 to 66–93%. Fluoride, cyanide, chloramphenicol, and rifampicin all caused similar increases. Aerobic growth on fermentable sugars resulted in CoASSG making up 40–55% of the CoA pool while growth on nonfermentable carbon sources or anaerobic fermentation resulted in CoASSG replacing acetyl CoA and free CoA to make up 85–95% of the CoA pool. The CoASSG:ATP ratio varied inversely with the growth rate in two groupings of carbon sources made up of either fermentable or nonfermentable molecules. Cultures grown aerobically on fermentable sugars exhibited a lower CoASSG:ATP ratio reflecting the lower proportion of CoASSG in the CoA pool.

scholarly journals Identification of Enzymatic Bottlenecks for the Aerobic Production of Malate from Glycerol by the Systematic Gene Overexpression of Anaplerotic Enzymes in Escherichia coli

2021 ◽  
Vol 22 (5) ◽  
pp. 2266
Author(s):  
Zamira E. Soto-Varela ◽  
Gema Cabrera ◽  
Agustin Romero ◽  
Domingo Cantero ◽  
Antonio Valle ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Anaerobic Fermentation ◽  
Waste Product ◽  
Carbon Sources ◽  
Dicarboxylic Acids ◽  
Tca Cycle ◽  
Biotechnological Production ◽  
Phosphoenol Pyruvate ◽  
Valuable Compounds ◽  
C4 Production

The biotechnological production of dicarboxylic acids (C4) from renewable carbon sources represents an attractive approach for the provision of these valuable compounds by green chemistry means. Glycerol has become a waste product of the biodiesel industry that serves as a highly reduced carbon source for some microorganisms. Escherichia coli is capable of consuming glycerol to produce succinate under anaerobic fermentation, but with the deletion of some tricarboxylic acid (TCA) cycle genes, it is also able to produce succinate and malate in aerobiosis. In this study, we investigate possible rate-limiting enzymes by overexpressing the C-feeding anaplerotic enzymes Ppc, MaeA, MaeB, and Pck in a mutant that lacks the succinate dehydrogenase (Sdh) enzyme. The overexpression of the TCA enzyme Mdh and the activation of the glyoxylate shunt was also examined. Using this unbiased approach, we found that phosphoenol pyruvate carboxylase (Ppc) overexpression enhances an oxidative pathway that leads to increasing succinate, while phosphoenol pyruvate carboxykinase (Pck) favors a more efficient reductive branch that produces mainly malate, at 57.5% of the theoretical maximum molar yield. The optimization of the culture medium revealed the importance of bicarbonate and pH in the production of malate. An additional mutation of the ppc gene highlights its central role in growth and C4 production.

Creation of Plasmidless and Markerless Escherichia coli Succinate Producing Strain and Evaluation of its Biosynthetic Potential during Utilization of Lignocellulosic Sugars

2020 ◽  
Vol 36 (2) ◽  
pp. 3-11
Author(s):  
O.A. Zhuravliova ◽  
Т.А. Voeikova ◽  
A.Yu. Gulevich ◽  
V.G. Debabov
Keyword(s):  
Escherichia Coli ◽  
Succinic Acid ◽  
Plant Biomass ◽  
Anaerobic Fermentation ◽  
Basic Research ◽  
Acid Fermentation ◽  
Mixed Acid ◽  
Gene Coding ◽  
Lignocellulosic Sugars ◽  
Basic Research Project

The plasmidless and markerless Escherichia coli succinate producing strain SGM2.0Pyc-int has been engineered and characterized. The strain has the inactivated main mixed-acid fermentation pathways due to the deletions of ldhA,poxB, ackA,pta, and adhE genes, constitutively expresses the genes of the aceEF-lpdA operon encoding components of pyravate dehydrogenase complex, and possesses the chromosomally integrated Bacillus subtilis pycA gene coding for pyruvate carboxylase. The capacity of the strain to synthesize succinic acid in course of dual-phase aerobic-anaerobic fermentation with lignocellulosic sugars as substrates was studied. The SGM2.0Pyc-int strain synthesized succinic acid from glucose, xylose, and arabinose with a molar yields of 1.41 mol/mol, 1.18 mol/mol, and 1.18 mol/mol, respectively, during the anaerobic production stage. The constructed strain has great potential for developing efficient processes for the succinic acid production from plant biomass-derived sugars. Escherichia coli, fermentation, arabinose, glucose, xylose, succinic acid. The work was supported by a Grant from the Russian Foundation for Basic Research (Project no. 18-29-14005).

scholarly journals Succinyl Coenzyme A Synthetase from Escherichia coli

1967 ◽  
Vol 242 (15) ◽  
pp. 3531-3537
Author(s):  
John A. Grunau ◽  
Ernest Knight ◽  
Emily S. Hart ◽  
I.C. Gunsalus
Keyword(s):  
Escherichia Coli ◽  
Coenzyme A ◽  
Succinyl Coenzyme A Synthetase

scholarly journals Mutants of Escherichia coli with Temperature-sensitive Malonyl Coenzyme A-Acyl Carrier Protein Transacylase

1974 ◽  
Vol 249 (23) ◽  
pp. 7468-7475
Author(s):  
Mark E. Harder ◽  
Ruth C. Ladenson ◽  
Steven D. Schimmel ◽  
David F. Silbert
Keyword(s):  
Escherichia Coli ◽  
Coenzyme A ◽  
Acyl Carrier Protein ◽  
Temperature Sensitive ◽  
Carrier Protein

Changes in Size of Intracellular Pools of Coenzyme A and Its Thioesters inEscherichia coliK-12 Cells to Various Carbon Sources and Stresses

1998 ◽  
Vol 62 (6) ◽  
pp. 1122-1128 ◽  
Author(s):  
Shigeru CHOHNAN ◽  
Hiroaki IZAWA ◽  
Hirofumi NISHIHARA ◽  
Yoshichika TAKAMURA
Keyword(s):  
Coenzyme A ◽  
Carbon Sources

scholarly journals The growth of Paracoccus halodenitrificans in a defined medium

1984 ◽  
Vol 30 (6) ◽  
pp. 837-840 ◽  
Author(s):  
Lawrence I. Hochstein ◽  
Geraldine A. Tomlinson
Keyword(s):  
Synthetic Medium ◽  
Carbon Sources ◽  
Defined Medium ◽  
Anaerobic Growth ◽  
Inorganic Salts ◽  
Vitamin B ◽  
Aerobic Growth ◽  
Methionine Biosynthesis ◽  
Dependent Pathway

A synthetic medium, consisting of inorganic salts and any of a number of carbon sources, supported the aerobic growth of Paracoccus halodenitrificans when supplemented with thiamine. The same medium plus an appropriate nitrogenous oxide supported anaerobic growth when additionally supplemented with methionine. The observation that vitamin B12 or betaine replaced methionine suggested that P. halodenitrificans had a defect in the cobalamin-dependent pathway for methionine biosynthesis, as well as the inability to synthesize betaine when growing anaerobically.

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