Simultaneous separation of halophilic proteins and nucleic acids after adsorption onto agarose gels

1977 ◽  
Vol 55 (8) ◽  
pp. 904-907 ◽  
Author(s):  
Alan Pater ◽  
Mary M. Pater
Keyword(s):  
Nucleic Acids ◽  
Ammonium Sulphate ◽  
Salt Concentration ◽  
Concentration Gradient ◽  
Halophilic Bacterium ◽  
Agarose Gel ◽  
Dilute Solutions ◽  
Agarose Gels ◽  
Simultaneous Separation

In dilute solutions, proteins and nucleic acids from the extremely halophilic bacterium Halobacterium cutirubrum remain soluble in 2.38 M ammonium sulphate and bind firmly to unsubstituted agarose gel columns. These macromolecules are clearly separated during elution with a decreasing salt concentration gradient.

scholarly journals Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels

1974 ◽  
Vol 139 (1) ◽  
pp. 157-162 ◽  
Author(s):  
S. L. Petrović ◽  
B. D. Šumonja ◽  
R. B. Vasiljević
Keyword(s):  
Nucleic Acids ◽  
Penicillium Chrysogenum ◽  
Rna Viruses ◽  
Gel Filtration ◽  
Trisodium Citrate ◽  
Gel Chromatography ◽  
Agarose Gel ◽  
Density Region ◽  
Double Stranded Rna ◽  
Agarose Gels

Double-stranded nucleic acids from a strain of Penicillium chrysogenum containing RNA viruses were isolated by agarose-gel filtration, and separated into DNA and double-stranded RNA fractions by agarose-gel chromatography in 2.5m-NaCl. The DNA fraction contained less than 1% alkali-labile polynucleotides, and sedimented homogeneously at 8–10S in alkaline sucrose gradients. In CsCl gradients it tended to band in the density region of 1.66–1.72g/ml. It had a `melting' temperature (Tm) of 75°C in 0.015m-NaCl–0.0015m-trisodium citrate, corresponding to 51.5mol% of G+C. The double-stranded RNA fraction did not contain detectable DNA. It could not band in CsCl up to a density of 1.78g/ml, and mainly consisted of a 14–15S RNA species with a Tm of 88.5°C in the above solvent, and a G+C content of 49.3 mol%.

Bifunctional Photobinding of Psoralen to Single Stranded Nucleic Acids

1973 ◽  
Vol 28 (7-8) ◽  
pp. 370-375 ◽  
Author(s):  
S Marciani ◽  
M. Terbojevich ◽  
F Dall 'Acqua ◽  
G. Rodighiero
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Nucleic Acids ◽  
Dilute Solutions ◽  
Base Pairs ◽  
Bacterial Dna ◽  
Disordered Structure ◽  
Concentrated Solution ◽  
Reduced Rate ◽  
Molecular Weight Of Dna

Abstract As psoralen and other furocoumarin derivatives, intercalated between two base pairs of native DNA, under irradiation at 365 nm form inter-strand cross-linkings as a consequence of bifunctional addition, the writers have investigated the ability of psoralen to give such bifunctional photo­ additions, too, with nucleic acids with disordered or partilly disordered structure (denatured DNA and r-RNA). On the basis of fluorimetric, light-scattering, viscosimetric measurements and of the renaturation ability of denatured bacterial DNA, certain results have been obtained. In addition to monofunctional photoadditions, psoralen can give bifunctional binding by irradiation at 365 nm both with denatured DNA and with r-RNA. However, when irradiation of denatured DNA in the presence of psoralen was performed in a concentrated solution (0.4%), the formation of bifunctional additions between two different strands was demonstrated by the increase (50%) of molecular weight of denatured DNA. However, when irradiation of denatured DNA was performed in more dilute solutions (0.1%), the bifunctional photoaddition of psoralen took place producing only bi­ functional additions in the same strand, very probably with the formation of loops, as has been shown by the absence of increase of molecular weight of DNA and by the more restricted structure assumed by the macromolecule, revealed by the light-scattering and viscosimetric measurements. The formation of these bifunctional additions was confirmed by the reduced rate of renaturation shown by denatured bacterial DNA after irradiation in the presence of psoralen. In the case of r-RNA, psoralen, when irradiated can form bifunctional additions only in the same strand.

In situ XRF analysis of Cs adsorption by the precipitation bands of Prussian blue analogues formed in agarose gels

2019 ◽  
Vol 34 (5) ◽  
pp. 979-985 ◽  
Author(s):  
Hisashi Hayashi ◽  
Yui Sato ◽  
Saya Aoki ◽  
Mao Takaishi
Keyword(s):  
Prussian Blue ◽  
Agarose Gel ◽  
X Ray ◽  
Agarose Gels ◽  
Xrf Analysis ◽  
Prussian Blue Analogues ◽  
Xrf Spectroscopy

The measurement of Cs adsorption by the precipitation bands of Mn-based Prussian blue analogues (PBAs), Co-based PBAs, and Prussian blue (PB), which were spontaneously formed in agarose gel, was carried out using in situ X-ray fluorescence (XRF) spectroscopy.

scholarly journals Interaction of poly-L-lysine with nucleic acids. V. Effect of salt concentration

Biopolymers ◽  
1969 ◽  
Vol 8 (1) ◽  
pp. 153-155 ◽  
Author(s):  
Kimiko Matsuo ◽  
Masamichi Tsuboi
Keyword(s):  
Nucleic Acids ◽  
Salt Concentration

SELECTIVE RETENTION AND FILTRATION OF BRAIN NUCLEIC ACIDS IN AGAROSE GELS

1974 ◽  
Vol 22 (3) ◽  
pp. 327-332 ◽  
Author(s):  
S. L. Petrović ◽  
J. J. Ivanuš ◽  
L. M. Rakić
Keyword(s):  
Nucleic Acids ◽  
Agarose Gels ◽  
Selective Retention

Voltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels

1993 ◽  
Vol 212 (1) ◽  
pp. 168-172 ◽  
Author(s):  
R. Barbieri ◽  
V. Izzo ◽  
M.A. Costa ◽  
G. Giudice ◽  
G. Duro
Keyword(s):  
Nucleic Acids ◽  
Voltage Gradient ◽  
Agarose Gels ◽  
Gradient Electrophoresis

scholarly journals Characterization and use of the potent ribonuclease inhibitor aurintricarboxylic acid for the isolation of RNA from animal tissues

1989 ◽  
Vol 263 (1) ◽  
pp. 73-80 ◽  
Author(s):  
A F Skidmore ◽  
T J C Beebee
Keyword(s):  
Nucleic Acids ◽  
Northern Blotting ◽  
Rat Tissues ◽  
Agarose Gel ◽  
Animal Tissues ◽  
Ribonuclease Inhibitor ◽  
Subsequent Removal ◽  
Aurintricarboxylic Acid ◽  
High Yields

Inclusion of aurintricarboxylic acid (ATA) in extraction buffers for the isolation of RNA from animal tissues resulted in high yields (0.5-2.0 mg/g of tissue) of undegraded material as judged by agarose-gel-electrophoretic analyses and Northern-blotting experiments. However, ATA bound to nucleic acids, forming stable complexes, and so we have established methods for spectrophotometric quantification of RNA in these coloured complexes, and for easy removal of sufficient ATA to leave RNA in a consistently hybridizable condition at the end of a purification. The use and subsequent removal of ATA was straightforward and gave satisfactory results for all rat tissues tested.

Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis

2005 ◽  
Vol 43 (8) ◽  
Author(s):  
Qing Huang ◽  
Wei-Ling Fu
Keyword(s):  
Comparative Analysis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Fluorescent Dyes ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Dna Staining

AbstractEthidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Safer nucleic acid stains, such as SYBR

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