Relationships between nucleoside triphosphate pyrophosphohydrolase activity and inosine triphosphate accumulation in human erythrocytes

1976 ◽  
Vol 54 (10) ◽  
pp. 843-847 ◽  
Author(s):  
Christian Sober ◽  
J. Frank Henderson ◽  
George Zombor ◽  
Ernest E. McCoy ◽  
Vernon Verhoef ◽  
...  
Keyword(s):  
Specific Activity ◽  
Human Erythrocytes ◽  
Nucleoside Triphosphate ◽  
Human Red Blood Cells ◽  
Nucleoside Triphosphate Pyrophosphohydrolase ◽  
Specific Activities ◽  
Two Parameters ◽  
Relationship Of ◽  
The Relationship

The relationship between nucleoside triphosphate pyrophosphohydrolase (NTPH) (EC 3.6.1.19) activity in erythrocyte lysates and accumulation of radioactive inosine triphosphate (ITP) in human erythrocytes incubated in vitro with [14C]hypoxanthine, was studied in 93 humans. When ITP accumulation, expressed as percentage of total radioactive nucleotides, was plotted against NTPH specific activity, an inverse relationship was found to exist. A continuous spectrum of NTPH specific activities and ITP accumulation values exists in the human population and the relationship between these two parameters follows the relationship of substrate concentration to enzyme activity predicted by Michaelis–Menten enzyme kinetics. One interpretation of these data is that the ITP concentration in human red blood cells is controlled by the degradation of ITP to IMP and pyrophosphate catalyzed by NTPH.

THE RELATIONSHIP BETWEEN CORTICOSTERONE SYNTHESIS FROM ENDOGENOUS PRECURSORS AND FROM ADDED RADIOACTIVE PRECURSORS BY RAT ADRENAL TISSUE IN VITRO

1966 ◽  
Vol 36 (3) ◽  
pp. 231-238 ◽  
Author(s):  
G. P. VINSON
Keyword(s):  
Specific Activity ◽  
Single Point ◽  
Biosynthetic Pathways ◽  
Activity Change ◽  
Adrenal Tissue ◽  
Specific Activities ◽  
The Relationship ◽  
Synthesis Of Hormones ◽  
Venous Plasma

SUMMARY A kinetic study of corticosterone recovered during incubation of rat adrenal tissue with [4-14C]progesterone and [16-3H]pregnenolone shows that both its 3H: 14C ratio and its specific activity change with time throughout incubation. Corticosterone is itself metabolized, and hypotheses based on the rate of production of corticosterone may be therefore deceptive when the amount is measured at a single point in time. Thus disparities between the specific activities of products and intermediates may be expected which alone do not necessarily indicate differences between the biosynthetic pathways involved in production from endogenous precursors and those from added radioactive precursors. Other experiments suggest that steroids in incubation media, in concentrations comparable with those found in adrenal venous plasma, do not inhibit the continued synthesis of hormones.

Specific Activities of the UTP Pools of Human Lymphocytes After Uridine-3H Labeling

1973 ◽  
Vol 51 (6) ◽  
pp. 954-957 ◽  
Author(s):  
T. Fields ◽  
L. Brox
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Rna Synthesis ◽  
Specific Activity ◽  
Human Lymphocytes ◽  
Direct Measure ◽  
Specific Activities ◽  
The Relationship

Human lymphocytes were labeled in vitro under various conditions with uridine-5-3H, and the specific activities of the acid-soluble UTP pools were determined by high-pressure liquid chromatography. Essentially, all the acid-soluble counts were in either UTP- or UDP-glucose. The relationship between the intracellular UTP specific activity, the extracellular uridine concentration, and the time of labeling demonstrates that the incorporation of uridine cannot be taken as a direct measure of RNA synthesis in these cells.

The Relationship of Width, Thickness, Volume and Load to Extension for Human Skin in Vitro

1980 ◽  
Vol 9 (4) ◽  
pp. 179-183 ◽  
Author(s):  
H L Stark ◽  
A Al-Haboubi
Keyword(s):  
Human Skin ◽  
Collagen Fibre ◽  
Phase Volume ◽  
Lateral Contraction ◽  
Low Stiffness ◽  
Phase Thickness ◽  
Relationship Of ◽  
The Relationship ◽  
Fibre Matrix

The relationships of width, thickness, volume and load to extension for human skin in vitro are reported. The specimens tested exhibited a low stiffness phase followed by a high stiffness phase. Volume rose than fell back to the initial volume at approximately the end of the low stiffness phase, and continued on falling to a final reduction of about 20 per cent at failure. Width decreased throughout, showing a maximum rate of reduction at approximately the end of the low stiffness phase. Thickness increased at a rate which also was maximum at the end of the low stiffness phase. The specimens used were long compared with their width and thickness thus offering no constraint to lateral contraction. An interpretation of this data in respect of the behaviour of the collagen fibre matrix is postulated.

Adrenocortical function in aging exercise-trained rats

1977 ◽  
Vol 43 (5) ◽  
pp. 839-843 ◽  
Author(s):  
J. A. Severson ◽  
R. D. Fell ◽  
J. G. Tuig ◽  
D. R. Griffith
Keyword(s):  
Age Groups ◽  
Plasma Corticosterone ◽  
Treadmill Running ◽  
Adrenocortical Function ◽  
Elevated Plasma ◽  
Corticosterone Concentration ◽  
Relationship Of ◽  
The Relationship

Plasma corticosterone concentrations and in vitro adrenal secretion of corticosterone were determined in exercise-trained rats. Rats, 100, 200, and 300 days of age, were trained for a 10-wk period by treadmill running. Following the training program, rats were subjected to an acute bout of swimming. Acute swimming elevated plasma corticosterone concentrations in all age groups. At 170 days of age, the plasma corticosterone concentration following swimming was higher in exercise-trained rats than in controls. The opposite was true of acutely swum rats at 270 and 370 days of age. Acute swimming elevated the in vitro adrenal gland response to adrenocorticotropic hormone stimulation in control rats at all ages and in trained rats at 170 days of age. The in vivo relationship of epinephrine and the pituitary adrenal system is suggested as a mechanism which could have caused this response. The relationship of secretion rates to plasma corticosterone concentrations indicated that extra-adrenal mechanisms, such as decreased turnover, were also responsible for the elevated plasma corticosterone levels observed in response to acute swimming.

The development of the lamb with particular reference to the alimentary tract

1964 ◽  
Vol 6 (2) ◽  
pp. 169-178 ◽  
Author(s):  
R. V. Large
Keyword(s):  
Body Weight ◽  
Alimentary Tract ◽  
Live Weight ◽  
The Body ◽  
Root Transformation ◽  
Feeding Regimes ◽  
Gut Fill ◽  
Relationship Of ◽  
The Relationship

1. Thirty Suffolk × Half bred lambs were slaughtered at the following ages: two twin lambs at birth and two singles and two twins at 1, 2, 3, 4, 6, 8 and 16 weeks of age.2. The following weights were recorded: live-weight immediately before slaughter; and carcass, head, skin, feet, alimentary tract, heart, liver, kidneys, lungs and trachea, and blood immediately afterwards.3. The alimentary tract was emptied and weighed in four separate parts; reticulo-rumen, omasum-abomasum, small intestine, large intestine.4. The volumes of the reticulo-rumen and the omasum-abomasum were measured by immersing in water and filling the organs with water to 2 cm. pressure.5. The in vitro digestive efficiency of rumen liquor from lambs of 1, 2, 3 and 4 weeks of age was assessed.6. Empty body weight was considered to be valuable in comparing animals of different ages or from different feeding regimes or at different times of the year because variations in gut ‘fill’ were eliminated.7. There were no differences between singles and twins in the relationship of the fresh weights of the parts of the body to empty body weight, except that development of the liver and the blood was rather slower for singles.8. Little evidence was found of a difference in rate of development of the alimentary tract between singles an d twins, although the log an d square root transformation suggested a possible difference in reticulo-rumen size in favour of twins, significant at the 5% level.

scholarly journals Ways of editorial art development

Bibliosphere ◽  
2016 ◽  
pp. 107-111
Author(s):  
L. A. Mosunova
Keyword(s):  
Functional Organization ◽  
Specific Activity ◽  
Productive Activity ◽  
Self Development ◽  
Relationship Of ◽  
The Relationship ◽  
Conditions Of Learning

Editorial art is treated as realization of the relationship of artistic-aesthetic and intellectual-speech activities. This specific activity is formed under certain conditions of learning editing, where it becomes a form of self-expression, conscience of students, means of their self-development. The principles of this training, the main one of which is the editing structural-functional organization in situation of joint productive activity, are described.

scholarly journals Higher gestational weight gain and lower serum estradiol levels on the day of human chorionic gonadotropin administration are associated with increased risk of preeclampsia after in vitro fertilization

2020 ◽  
Author(s):  
Yu-Chen Chen ◽  
Yun-Ju Lai ◽  
Yu-Ting Su ◽  
Ni-Chin Tsai ◽  
Kuo-Chung Lan
Keyword(s):  
Weight Gain ◽  
In Vitro Fertilization ◽  
Gestational Weight Gain ◽  
Chorionic Gonadotropin ◽  
Serum Estradiol ◽  
Vitro Fertilization ◽  
Gestational Weight ◽  
Relationship Of ◽  
The Relationship

Abstract Background Very few previous studies have examined the effect of endocrine parameters during ART on preeclampsia. Moreover, there is little known about the relationship of steroid hormone levels on development of the placenta. The purpose of this study is to assess the association of preeclampsia with serum estradiol (E2) and progesterone (P4) levels on the day of human chorionic gonadotropin (hCG) administration during controlled ovarian hyperstimulation (COH) for in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). Methods This was a hospital-based cohort study using clinical data from the Kaohsiung Chang Gung Memorial Hospital Obstetric and Neonatal Database (KCGMHOND) from Jan 1, 2001 to December 1, 2018. Eligible women underwent at least one autologous IVF/ICSI cycle and had a live-born infant with a gestational age (GA) of more than 20 weeks. Results A total of 622 women who had live births after fresh IVF/ICSI-ET during the study period met our inclusion criteria. Twenty-eight women (4.5%) met the diagnostic criteria for preeclampsia. However, women in the preeclampsia group had a significantly higher body mass index (22 vs. 24, p =0.05), body weight at delivery (70.0 vs. 80.5 kg, p <0.001) and gestational weight gain (13.0 vs. 19.6 kg, p =0.002) and had lower use of ICSI (29.9% vs. 10.7%, p =0.021). Logistic regression analysis of the relationship of patient and treatment characteristics with preeclampsia. The crude ORs indicated that young female age >34, not using ICSI, E2 peak <1200 pg/mL and gestational weight gain >20 kg were associated with preeclampsia. After adjustment for confounding, the only factors that remained significant were E2 peak <1200 pg/mL (aOR = 4.634, 95% CI = 1.061 to 20.222), and gestational weight gain >20 kg (aOR: 13.601, 95% CI: 3.784, 48.880). Conclusions For women receiving IVF/ICSI, lower estradiol hormone levels on the day of hCG administration and higher pregnancy weight gain are related with subsequent preeclampsia.

Lethality of a tritiated amino acid in early mouse embryos

Development ◽  
1985 ◽  
Vol 88 (1) ◽  
pp. 209-217
Author(s):  
Janet L. Wiebold ◽  
Gary B. Anderson
Keyword(s):  
Specific Activity ◽  
Subsequent Development ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Radiation Induced ◽  
Specific Activities ◽  
Tritiated Amino Acids ◽  
Early Mouse

2- to 4-cell and morula- to blastocyst-stage mouse embryos were cultured for 1 h in tritiated leucine at two specific activities and their subsequent development followed in vitro and in vivo (after transfer to recipients), respectively. 2- to 4-cell embryos that incorporated an average of 42 d.p.m. per embryo were impaired in their ability to develop to the morula and blastocyst stage. Recipients receiving morulae and blastocysts that had incorporated an average of 384 d.p.m. per embryo failed to produce young. Reduction of the specific activity improved the viability of embryos both in vitro and in vivo but development was still less than that of unlabelled embryos. Protein degradation curves were different for both 2- to 4-cell and morulato blastocyst-stage embryos labelled at the two different specific activities. Most studies using tritiated amino acids have employed higher specific activities than those used here and they may have to be reevaluated due to the possibility of radiation-induced artifacts.

scholarly journals On the Nature of Castle’s Hemopoietic Factor

Blood ◽  
1951 ◽  
Vol 6 (12) ◽  
pp. 1234-1239 ◽  
Author(s):  
SHEILA T. CALLENDER ◽  
L. G. LAJTHA
Keyword(s):  
Vitamin B12 ◽  
Gastric Juice ◽  
Intrinsic Factor ◽  
Normal Serum ◽  
Relationship Of ◽  
The Relationship

Abstract 1. Normal gastric juice (intrinsic factor) and vitamin B12 together form a thermolabile hemopoietic factor which ripens megaloblasts in vitro, both gastric juice and B12 alone being inactive. 2. The hemopoietic factor in normal serum which ripens megaloblasts in vitro also appears to be thermolabile, heating to 56 C. for 2 hours destroying some of its activity. 3. The relationship of these factors is discussed and an extra-gastric as well as a gastric source of intrinsic factor is postulated.

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