Isolation and Tissue Specificity of Chromatin Associated Proteins in Vicia faba

1975 ◽  
Vol 53 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Barbara Grahek Mischke ◽  
Oscar G. Ward
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Vicia Faba ◽  
Tissue Specificity ◽  
Gene Action ◽  
Sodium Dodecyl ◽  
Acid Extraction ◽  
Dodecyl Sulfate ◽  
Associated Proteins ◽  
Different Tissues

A method is described that permits extraction of one class of non-histones in 8 M urea −0.14 M mercaptoethanol prior to acid extraction of histones and a second class in 0.05 M Tris – 1% sodium dodecyl sulfate following acid extraction of histones. Comparisons of histones and non-histones extracted by this method with those obtained by other procedures demonstrate two important advantages of the method: (1) histones obtained by this method are not contaminated by acid-soluble non-histones, and (2) non-histones are not subjected to acid or phenol during extraction. Changes in the distributions of chromatin-associated proteins in different tissues suggest that some species represent regulators of gene action.

Solubility and adsorption behaviors of chlorophenols in the presence of surfactant

1997 ◽  
Vol 35 (7) ◽  
pp. 123-130 ◽  
Author(s):  
J. C. Liu ◽  
P. S. Chang
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Critical Micelle Concentration ◽  
Sodium Dodecyl ◽  
Adsorption Behaviors ◽  
Brij 35 ◽  
Important Index ◽  
Dodecyl Sulfate ◽  
Triton X

The solubility of chlorophenols as affected by surfactant was investigated. Three kinds of surfactant, sodium dodecyl sulfate, Triton X-100, and Brij 35, were utilized. The solubilization of chlorophenols by surfactant follows the order of 2,4,6-trichlorophenol > 2,4-dichlorophenol > 2,6-dichlorophenol > 2-chlorophenol; and the critical micelle concentration is an important index. The adsorption reactions of 2,4-dichlorophenol and 2,4,6- trichlorophenol onto hydrous montmorillonite in the presence of surfactant were examined. The presence of surfactant decreased the adsorption of chlorophenols significantly. The roles of hydrophobicity of chlorophenols in solubilization and adsorption behaviors are discussed.

Different submicellar solubilization mechanisms revealed by 1H NMR and 2D diffusion ordered spectroscopy (DOSY)

2020 ◽  
Vol 22 (19) ◽  
pp. 11075-11085
Author(s):  
Mengjian Wu ◽  
Zhaoxia Wu ◽  
Shangwu Ding ◽  
Zhong Chen ◽  
Xiaohong Cui
Keyword(s):  
Nmr Spectroscopy ◽  
Sodium Dodecyl Sulfate ◽  
1H Nmr ◽  
Sodium Dodecyl ◽  
Butyl Methacrylate ◽  
H Nmr ◽  
Molecular Scale ◽  
Two Systems ◽  
Dodecyl Sulfate ◽  
Triton X

Different submicellar solubilization mechanisms of two systems, Triton X-100/tetradecane and sodium dodecyl sulfate (SDS)/butyl methacrylate, are revealed on the molecular scale by 1H NMR spectroscopy and 2D diffusion ordered spectroscopy (DOSY).

New Insights into the Kinetics and Morphology of CO2 Hydrate Formation in the Presence of Sodium Dodecyl Sulfate

2021 ◽  
Author(s):  
Xiao-Yan Deng ◽  
Ying Yang ◽  
Dong-Liang Zhong ◽  
Xi-Yue Li ◽  
Bin-Bin Ge ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Hydrate Formation ◽  
Dodecyl Sulfate
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