A Flow Method for Determination of the Kinetic Parameters for Immobilized Enzymes

1975 ◽  
Vol 53 (1) ◽  
pp. 11-14 ◽  
Author(s):  
T. T. Ngo ◽  
P. S. Bunting ◽  
K. J. Laidler
Keyword(s):  
Kinetic Parameters ◽  
Flow Rate ◽  
Immobilized Enzymes ◽  
Flow Cell ◽  
Substrate Solution ◽  
Flow Method ◽  
Inner Surface ◽  
Electric Eel ◽  
Nylon Tubing

A flow method is described for determination of the kinetic parameters (Vm and Km) for enzymes that are bound to particles, to membranes, and to the interior surfaces of tubes. Substrate solution is pumped through Tygon tubing to a microvolume flow cell and back into the reaction mixture, the flow rate being adjusted to be faster than the rate of formation of product. To illustrate the technique, it is applied to the determination of the parameters for electric-eel acetylcholinesterase attached to particles, to membranes, and to the inner surface of nylon tubing.

An apparatus for the determination of kinetic parameters associated with immobilized enzymes

1973 ◽  
Vol 55 (1) ◽  
pp. 282-287 ◽  
Author(s):  
François Widmer ◽  
Jack E. Dixon ◽  
Nathan O. Kaplan
Keyword(s):  
Kinetic Parameters ◽  
Immobilized Enzymes

Discussion of Problems of Measurement Carbon on the Inner Surface of Inner Grooved Copper Tube by Infrared Absorption Method

2012 ◽  
Vol 503-504 ◽  
pp. 1347-1350
Author(s):  
Huai Bao Yuan ◽  
Yue Hong Li
Keyword(s):  
Flow Rate ◽  
Infrared Absorption ◽  
Copper Tube ◽  
Absorption Method ◽  
Calibration Constant ◽  
Integral Calculus ◽  
Surface Carbon ◽  
Inner Surface ◽  
Recovery Experiment

The inner surface carbon in the inner grooved copper tube was determined by tube furnace heating-infrared absorption method. Some factors which influenced the determination such as sample preparation and cleaning, flow rate, furnace constants, blank value, calibration constant were tested and optimized. The inner surface carbon of copper tube was released completely while the experiment temperature was chosen as 400~600°C and the flow rate of oxygen was 750 mL/min. And the obtained integral calculus curve was ideal. The method has been applied to the determination of inner surface carbon in the inner grooved copper tube with carbon content of 0.24 mg/dm2. The result of the RSD was 3.7％. And the recovery experiment through joining in synthesize carbon sample was carried on, the recovery was 98～103%.

MEASUREMENT OF STEROID BINDING PROTEINS

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S104-S121 ◽  
Author(s):  
E. E. Baulieu ◽  
J. P. Raynaud ◽  
E. Milgrom
Keyword(s):  
Kinetic Parameters ◽  
Binding Proteins ◽  
Binding Specificity ◽  
Binding Parameters ◽  
Target Organs ◽  
Biological Mixtures ◽  
Steroid Binding Proteins ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.

DETERMINATION OF THE LIMITING FLOW RATE IN LIQUID FILMS FOR HEAT TRAHSFER CRISIS CALCULATION

1978 ◽  
Author(s):  
V. M. Borishansky ◽  
A.A. Andreevsky ◽  
Mikhail Ya. Belenkiy ◽  
G.S Bykov ◽  
Mikhail Gotovskii ◽  
...  
Keyword(s):  
Flow Rate ◽  
Liquid Films
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