Excision Properties of DNA Polymerase

1973 ◽  
Vol 51 (10) ◽  
pp. 1399-1401
Author(s):  
L. M. Hoepfinger ◽  
C. D. Morgan ◽  
C. L. Lakanen
Keyword(s):  
Escherichia Coli ◽  
Acridine Orange ◽  
Dna Polymerase ◽  
Nitrous Acid ◽  
Dna Polymerase I ◽  
Untreated Material ◽  
Polymerase I

The excision of nucleotides from altered DNA or d(A-T) copolymer by Escherichia coli DNA polymerase I was measured. Altered DNA or d(A-T) copolymer was produced by treating radioactive DNA or d(A-T) copolymer with nitrous acid, formaldhehyde, or acridine orange. The resultant products were subjected to DNA polymerase I treatment. Excision of nucleotides occurred to a greater extent from the nitrous acid and formaldehyde treated materials than it did from untreated material. DNA treated with acridine orange snowed no significant differences in excision relative to untreated DNA.

scholarly journals The effects of acridine orange on deoxyribonucleic acid in Escherichia coli

1978 ◽  
Vol 171 (3) ◽  
pp. 567-573 ◽  
Author(s):  
G R Barker ◽  
N Hardman
Keyword(s):  
Escherichia Coli ◽  
Acridine Orange ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Insoluble Fraction ◽  
Low Molecular Weight ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Dna Polymerase I ◽  
Polymerase I

1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA isolated from cells treated with Acridine Orange under conditions that inhibit growth contains material of low molecular weight, which is absent from DNA isolated from cells treated under conditions in which growth is not impaired. 4. Cells incubated with Acridine Orange at both pH 7.4 and 7.9 suffer degradation of DNA, as shown by loss of labelled DNA from the acid-insoluble fraction, which is not observed with untreated cells at either pH. 5. The results suggest that elimination of the F'-Lac plasmid by Acridine Orange requires inactivation of repair processes.

Sign in / Sign up

Export Citation Format

Share Document