Attempts to Find a Specific Intranuclear Location for Replicating Polyoma Virus DNA

1973 ◽  
Vol 51 (9) ◽  
pp. 1225-1228 ◽  
Author(s):  
E. Frost ◽  
P. Bourgaux
Keyword(s):  
Nuclear Membrane ◽  
Mouse Embryo ◽  
Protein Complexes ◽  
Polyoma Virus ◽  
Large Structure ◽  
Viral Dna ◽  
Embryo Cells

Attempts were made to localize the site of replication of polyoma virus DNA in mouse embryo cells. Although newly synthesized viral DNA was clearly confined within the nucleus, no evidence was found to indicate that replication takes place either on the nuclear membrane, or in the nucleolus, or in association with any other large structure. In all nuclear fractions examined, the mature and replicative forms of the viral DNA were found in the same proportions, but as parts of DNA–protein complexes with unique sedimentation properties.

RNA synthesis in polyoma virus-infected cells. I. Pattern of formation of polyribosome-associated messenger RNA during productive infection

1970 ◽  
Vol 48 (10) ◽  
pp. 1104-1112 ◽  
Author(s):  
William P. Cheevers ◽  
Rose Sheinin
Keyword(s):  
Dna Replication ◽  
Messenger Rna ◽  
Rna Synthesis ◽  
Polyoma Virus ◽  
Productive Infection ◽  
Mrna Synthesis ◽  
Viral Dna ◽  
Experimental Conditions ◽  
Embryo Cells ◽  
Infected Cells

Experimental conditions have been established for selective measurement of the synthesis of mRNA in mouse embryo cells. Using such conditions, it was found that productive infection of these cells by polyoma virus resulted in stimulation of mRNA synthesis. The pattern of induction of mRNA synthesis was biphasic, characterized by distinct "early" and "late" periods, as denoted by the time of initiation of progeny viral DNA replication. The formation of "early" mRNA was first detected at 9–11 h postinfection, 6 h prior to the time of onset of virus-induced synthesis of cell DNA and 9 h prior to initiation of polyoma DNA replication. The initiation of synthesis of "late" mRNA was approximately coincident with the onset of formation of viral DNA. Most of the newly synthesized "early" and "late" mRNA was of relatively small size (8–12 S) and was associated with polyribosomes which sedimented at less than 180 S. The proportion of the total "late" mRNA which was virus-specific was three times higher than that of the total "early" mRNA; however, the mRNA synthesized both "early" and "late" was predominantly cell-specific.

Activity of Thymidine Kinase in Mouse Embryo Cells infected with Polyoma Virus

Nature ◽  
1967 ◽  
Vol 214 (5084) ◽  
pp. 191-192 ◽  
Author(s):  
G. RICCERI ◽  
G. COCUZZA
Keyword(s):  
Thymidine Kinase ◽  
Mouse Embryo ◽  
Polyoma Virus ◽  
Embryo Cells

scholarly journals Effect of Phleomycin on Polyoma Virus Synthesis in Mouse Embryo Cells

1971 ◽  
Vol 7 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Akiko Iwata ◽  
R. A. Consigli
Keyword(s):  
Mouse Embryo ◽  
Polyoma Virus ◽  
Embryo Cells

Rosette-Like Arrangement of Polyoma Virus Particles in Infected Mouse-Embryo Cells

1965 ◽  
Vol 89 (2) ◽  
pp. 535-536
Author(s):  
Ryoichi Mori ◽  
Kazunobu Amako ◽  
Kenji Takeya
Keyword(s):  
Infected Mouse ◽  
Mouse Embryo ◽  
Polyoma Virus ◽  
Virus Particles ◽  
Embryo Cells
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