Chain Termini of the Satellite RNA from Yeast Ribosomes

1973 ◽  
Vol 51 (5) ◽  
pp. 520-528 ◽  
Author(s):  
K. M. Oakden ◽  
B. G. Lane
Keyword(s):  
Group Analysis ◽  
Formal Structure ◽  
Yeast Cells ◽  
Satellite Rna ◽  
Dominant Form ◽  
Selective Precipitation ◽  
Specific Complex ◽  
Yeast Ribosomes ◽  
Electrophoretic Component ◽  
End Group

(1) When yeast cells are extracted with aqueous phenol and the aqueous phase is made 2.5 M with respect to NaCl at 0°, there is selective precipitation of about 80% of the total RNA. The 17 S and 26 S RNA from yeast ribosomes comprise a preponderant mass-fraction (ca. 90%) of this NaCl-insoluble RNA. A small amount (ca. 3%) of a rapidly migrating electrophoretic component (iRMEC) can be released by aqueous denaturation of yeast NaCl-insoluble RNA (iRNA), and because it forms a specific complex with 26 S RNA, this iRMEC component can be appropriately described as a "satellite" of 26 S RNA.(2) Following its release by aqueous denaturation of yeast NaCl-insoluble RNA, the satellite RNA has been subjected to end-group analysis, and it has been found to have a formal structure that is based on a repeating 5′-mononucleotide unit, i.e. (pN)n, where n = 160–200. On the basis of an analysis of its principal termini, the dominant form of the satellite RNA is[Formula: see text](3) In an allied study, a "rapidly labelled" fraction of yeast NaCl-insoluble RNA has been subjected to aqueous denaturation and end-group analysis.

The 3′-hydroxyl termini in yeast ribosomal RNA

1970 ◽  
Vol 48 (10) ◽  
pp. 1113-1121 ◽  
Author(s):  
K. M. Olver ◽  
B. G. Lane
Keyword(s):  
Gradient Centrifugation ◽  
Group Analysis ◽  
Ribosomal Subunit ◽  
Sucrose Density Gradient ◽  
Sucrose Density Gradient Centrifugation ◽  
Selective Precipitation ◽  
Ribosomal Subunits ◽  
Hydroxyl Termini ◽  
End Group ◽  
Total Yeast

(1) A fraction of high molecular weight RNA was prepared by subjecting total yeast RNA to selective precipitation from aqueous 2.5 M sodium chloride solution at 0°. This "insoluble" RNA accounted for about 80% of the total yeast RNA.(2) The "insoluble" RNA and various of its subfractions obtained by preparative sucrose density-gradient centrifugation were subjected to electrophoretic and end-group analyses.(3) One subfraction, largely composed of 17 S RNA from the smaller ribosomal subunit, was found to contain adenosine as the principal 3′-hydroxyl terminal nucleoside residue. Based on the analysis of 3′-hydroxyl termini, RNA in this subfraction had a mean residue length of about 1600 nucleotides.(4) Another subfraction, largely composed of 26 S RNA from the larger ribosomal subunit, was found to contain uridine as the principal 3′-hydroxyl terminal nucleoside residue. Based on the analysis of 3′-hydroxyl termini, RNA in this subfraction had a mean residue length of about 2400 nucleotides.(5) Electrophoretic and end-group analyses of other subfractions have been reported and discussed in terms of the possible origin of the RNA in these fractions, and also in terms of the way in which the RNA of these fractions might tend to affect the end-group analysis of both the parent fraction and the principal subfractions, which contain RNA from the ribosomal subunits.

Wheat-Embryo Ribonucleates. I. Subcellular Localization of a Satellite Polyribonucleotide

1973 ◽  
Vol 51 (5) ◽  
pp. 606-612 ◽  
Author(s):  
A. A. Azad ◽  
B. G. Lane
Keyword(s):  
Microsomal Fraction ◽  
Large Subunit ◽  
Satellite Rna ◽  
Selective Precipitation ◽  
Ribosomal Subunits ◽  
Wheat Embryo ◽  
Wheat Embryos ◽  
The Subject ◽  
Rna Complex ◽  
Electrophoretic Component

(1) When wheat embryos are extracted with aqueous phenol and the aqueous phase is made 2.5 M with respect to NaCl at 0 °C, there is selective precipitation of about 80% of the total RNA. The 18 S and 26 S RNA species from the wheat-embryo ribosomes comprise a preponderant mass fraction (ca. 80%) of this NaCl-insoluble RNA (iRNA). A small amount of a rapidly migrating electrophoretic component (iRMEC) can be released by aqueous denaturation of wheat-embryo NaCl-insoluble RNA and because it is specifically complexed with 26 S RNA, the iRMEC component has been termed a "satellite" of 26 S RNA.(2) The wheat-embryo satellite RNA has been shown to be present in the microsomal fraction recovered from cell-free homogenates of wheat embryos.(3) The wheat-embryo satellite RNA has been shown to be differentially localized in the large subunit of wheat-embryo ribosomes where it presumably exists as part of the same intermolecular 26 S RNA complex that can be isolated by directly extracting the whole embryos with aqueous phenol.(4) During preparation of the ribosomal subunits, there is substantial degradation of the component ribonucleates and the nature of this degradation is the subject of a brief discussion.

scholarly journals THE ISOTOPE DERIVATIVE METHOD OF PROTEIN AMINO END-GROUP ANALYSIS

1951 ◽  
Vol 190 (2) ◽  
pp. 733-740 ◽  
Author(s):  
Sidney. Udenfriend ◽  
Sidney F. Velick
Keyword(s):  
Group Analysis ◽  
Derivative Method ◽  
End Group

scholarly journals Genetic Polymorphism in Caseins of Cow's Milk. V. End-Group Analysis of β-Caseins A, B, and C

1965 ◽  
Vol 48 (7) ◽  
pp. 884-887 ◽  
Author(s):  
E.B. Kalan ◽  
M.P. Thompson ◽  
Rae Greenberg ◽  
L. Pepper
Keyword(s):  
Genetic Polymorphism ◽  
Group Analysis ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
End Group

scholarly journals Sizing and Thermal Stability of Prepared Tetraaminophthalocyaninatocopper(II) Derivatives-grafted Polymers

2016 ◽  
Vol 13 (2) ◽  
pp. 221-234
Author(s):  
Baghdad Science Journal
Keyword(s):  
Thermal Stability ◽  
Ethylene Glycol ◽  
Group Analysis ◽  
Condensation Polymerization ◽  
Grafted Polymers ◽  
Poly Ethylene Glycol ◽  
Molecular Weights ◽  
Poly Ethylene ◽  
Thermal Stability Of ◽  
End Group

Different polymers were prepared by condensation polymerization of sebacic anhydride and adipic anhydride with ethylene glycol and poly(ethylene glycol). Their number average molecular weights were determined by end group analysis. Then, they were grafted on the prepared phthalocyaninatocopper(II) compounds with the general formula (NH2)4PcCu(II) having amino groups of 3,3',3'',3'''- or 4,4',4'',4'''- positions. All prepared polymers, compounds, and phthalocyaninatocopper(II)-grafted polymers were characterized by FTIR. The sizing measurements were carried out in 3,3',3'',3'''- (NH2)4PcCu(II) and 4,4',4'',4'''- (NH2)4PcCu(II) compounds with and without grafting polymers. The results showed that the grafting process led to decreasing in particle size and increasing in surface area. The grafting process was reflected positively on the thermal degradation of 3,3',3'',3'''- (NH2)4PcCu(II) and 4,4',4'',4'''- (NH2)4PcCu(II) grafted polymers. They had higher thermal stability accompanied with higher char residue and T50% weight loss with 3,3',3'',3'''-(NH2)4PcCu(II) and their grafted polymers being the best.

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