Isolation of the Bromouracil-Substituted Light Strand of Mouse Satellite DNA

1973 ◽  
Vol 51 (1) ◽  
pp. 7-10 ◽  
Author(s):  
I. G. Walker ◽  
D. F. Ewart ◽  
A. Chan
Keyword(s):  
Satellite Dna ◽  
Buoyant Density ◽  
Cesium Chloride ◽  
Nucleotide Composition ◽  
Published Data ◽  
Base Pairing ◽  
Distinct Peak ◽  
Double Stranded Dna ◽  
Mouse Satellite Dna ◽  
Light Strand

Mouse L-cells were grown in medium containing bromodeoxyuridine and fluorodeoxyuridine, and the DNA from these cells was centrifuged to equilibrium in alkaline cesium chloride. The bulk of the bromouracil-substituted DNA had a buoyant density lying between 1.82 and 1.85 g/cc. About 4–5% of bromouracil-substituted DNA was observed as a distinct peak with a buoyant density of 1.79 g/cc. After this DNA was purified by recentrifuging, nucleotide analysis revealed an asymmetric composition in which the base-pairing rules for double-stranded DNA were not obeyed. Comparison of the nucleotide composition with published data indicated that the bromouracil-substituted light strand of mouse satellite DNA had been isolated.

A phage-like bacteriocin of Rhizobium trifolii

1973 ◽  
Vol 19 (3) ◽  
pp. 359-368 ◽  
Author(s):  
E. A. Schwinghamer ◽  
C. E. Pankhurst ◽  
P. R. Whitfeld
Keyword(s):  
Phage Type ◽  
Buoyant Density ◽  
Cesium Chloride ◽  
Close Correlation ◽  
Ultraviolet Absorption Spectrum ◽  
Bacterial Cells ◽  
Rhizobium Trifolii ◽  
Bacteriocin Activity ◽  
Double Stranded Dna ◽  
Defective Phage

An inducible bacteriocin produced by a strain of Rhizobium trifolii was partially purified by ammonium sulfate precipitation and differential centrifugation. Examination of fractions of this material banded in sucrose gradients showed a close correlation between bacteriocin activity and presence of phage-like particles observed by electron microscopy. The particles have a head diameter of about 50 nm and have short tails. Buoyant density of intact particles in cesium chloride was 1.46 g/cm3, as compared with 1.49 g/cm3 for a reference rhizobiophage. The apparent nucleoprotein nature of the bacteriocin was confirmed by the ultraviolet absorption spectrum of CsCl-banded material. Double-stranded DNA of density 1.720 g/cm3 (compared with 1.716 g/cm3 for DNA of the phage) was identified in bacteriocin fractions obtained from CsCl density equilibrium gradients. This density was identical with that of DNA from both induced and noninduced bacterial cells of the producing strain. These characteristics and the inability to reproduce in sensitive bacteria identify the bactericidal agent as a large bacteriocin of the defective phage type. Some apparent differences between this bacteriocin and other, partly characterized bacteriocins of R. trifolii are discussed.

scholarly journals Genomic Tackling of Human Satellite DNA: Breaking Barriers through Time

2021 ◽  
Vol 22 (9) ◽  
pp. 4707
Author(s):  
Mariana Lopes ◽  
Sandra Louzada ◽  
Margarida Gama-Carvalho ◽  
Raquel Chaves
Keyword(s):  
Human Genome ◽  
Satellite Dna ◽  
Repetitive Sequences ◽  
Nucleotide Composition ◽  
Genomic Component ◽  
Genomic Studies ◽  
Human Genomic ◽  
Definition Of ◽  
High Degree

(Peri)centromeric repetitive sequences and, more specifically, satellite DNA (satDNA) sequences, constitute a major human genomic component. SatDNA sequences can vary on a large number of features, including nucleotide composition, complexity, and abundance. Several satDNA families have been identified and characterized in the human genome through time, albeit at different speeds. Human satDNA families present a high degree of sub-variability, leading to the definition of various subfamilies with different organization and clustered localization. Evolution of satDNA analysis has enabled the progressive characterization of satDNA features. Despite recent advances in the sequencing of centromeric arrays, comprehensive genomic studies to assess their variability are still required to provide accurate and proportional representation of satDNA (peri)centromeric/acrocentric short arm sequences. Approaches combining multiple techniques have been successfully applied and seem to be the path to follow for generating integrated knowledge in the promising field of human satDNA biology.

Molecular Hybridization of Mouse Satellite DNA-Complementary RNA in Ultrathin Sections Prepared for Electron Microscopy

1974 ◽  
Vol 14 (2) ◽  
pp. 253-261
Author(s):  
J. JACOB ◽  
KATHERINE GILLIES ◽  
D. MACLEOD ◽  
K. W. JONES
Keyword(s):  
Electron Microscopy ◽  
Satellite Dna ◽  
Similar Distribution ◽  
Tissue Sections ◽  
Satellite Sequences ◽  
Interphase Cells ◽  
Nucleolar Chromatin ◽  
Ultrathin Sections ◽  
Mouse Satellite Dna

The feasibility of in situ hybridization in tissue sections prepared for electron microscopy has been examined using mouse satellite DNA-complementary RNA and mouse L cells. The results obtained are encouraging, although certain technical aspects require further clarification. In interphase cells, hybrid-forming sites occur in chromatin patches positioned along the nuclear envelope. It is also confirmed that satellite DNA occurs in nucleolus-associated chromatin. The results suggest that satellite sequences are present in intranucleolar and peri-nucleolar chromatin. A similar distribution is indicated for ribosomal cistrons.

scholarly journals Nudeotide sequence of mouse satellite DNA

1981 ◽  
Vol 9 (3) ◽  
pp. 683-696 ◽  
Author(s):  
Wolfram Hörz ◽  
Werner Altenburger
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

The organization of the mouse satellite DNA at centromeres

1989 ◽  
Vol 183 (2) ◽  
pp. 494-500 ◽  
Author(s):  
Ann Joseph ◽  
A.R. Mitchell ◽  
O.J. Miller
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

A NON-NUCLEOTIDE POLYMER FOUND IN THE DNA OF THE SAND DOLLAR, ECHINARACHNIUS PARMA: II. PRELIMINARY CHARACTERIZATION

1967 ◽  
Vol 45 (2) ◽  
pp. 281-287 ◽  
Author(s):  
Herbert S. Rosenkranz
Keyword(s):  
Buoyant Density ◽  
Sedimentation Coefficient ◽  
Negative Electrode ◽  
Cesium Chloride ◽  
Amino Sugar ◽  
Electrophoretic Analysis ◽  
Positive Test ◽  
Preliminary Characterization ◽  
Acid Hydrolysate

A preliminary characterization of the non-nucleotidic component present in the DNA of Echinarachnius parma was undertaken. This material has an extremely high sedimentation coefficient (907 S). It contains no deoxyribose and presumably no ribose. After acid hydrolysis it was strongly ninhydrin-positive and also gave positive tests for reducing sugars as well as a slightly positive test for amino sugars. Upon electrophoretic analysis of an acid hydrolysate, three ninhydrinpositive spots were detected. One of these migrated to the negative electrode with a mobility identical with that of galactosamine, the other migrated to the positive electrode, and the third was neutral at pH 6.3. The spot with a mobility identical with that of galactosamine also gave a positive test for amino sugar. The material was not attacked by α-amylase. However, digestion with a crude trypsin preparation resulted in loss of the banding property in gradients of cesium chloride. Exposure to purified trypsin did not completely digest it, but caused an increase in buoyant density.

scholarly journals Microtubule-associated protein MAP2 preferentially binds to a dA/dT sequence present in mouse satellite DNA.

1983 ◽  
Vol 2 (8) ◽  
pp. 1229-1234 ◽  
Author(s):  
J. Avila ◽  
E. Montejo de Garcini ◽  
F. Wandosell ◽  
A. Villasante ◽  
J.M. Sogo ◽  
...  
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

scholarly journals Mouse Satellite DNA

Nature ◽  
1967 ◽  
Vol 215 (5101) ◽  
pp. 575-575
Author(s):  
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

scholarly journals Mouse satellite DNA, centromere structure, and sister chromatid pairing.

1986 ◽  
Vol 103 (4) ◽  
pp. 1145-1151 ◽  
Author(s):  
L M Lica ◽  
S Narayanswami ◽  
B A Hamkalo
Keyword(s):  
Electron Microscopy ◽  
Sister Chromatid ◽  
Satellite Dna ◽  
Marker Chromosome ◽  
Restriction Enzymes ◽  
Centromeric Heterochromatin ◽  
Mitotic Chromosomes ◽  
Metaphase Chromosomes ◽  
Sister Chromatids ◽  
Mouse Satellite Dna

The experiments described were directed toward understanding relationships between mouse satellite DNA, sister chromatid pairing, and centromere function. Electron microscopy of a large mouse L929 marker chromosome shows that each of its multiple constrictions is coincident with a site of sister chromatid contact and the presence of mouse satellite DNA. However, only one of these sites, the central one, possesses kinetochores. This observation suggests either that satellite DNA alone is not sufficient for kinetochore formation or that when one kinetochore forms, other potential sites are suppressed. In the second set of experiments, we show that highly extended chromosomes from Hoechst 33258-treated cells (Hilwig, I., and A. Gropp, 1973, Exp. Cell Res., 81:474-477) lack kinetochores. Kinetochores are not seen in Miller spreads of these chromosomes, and at least one kinetochore antigen is not associated with these chromosomes when they were subjected to immunofluorescent analysis using anti-kinetochore scleroderma serum. These data suggest that kinetochore formation at centromeric heterochromatin may require a higher order chromatin structure which is altered by Hoechst binding. Finally, when metaphase chromosomes are subjected to digestion by restriction enzymes that degrade the bulk of mouse satellite DNA, contact between sister chromatids appears to be disrupted. Electron microscopy of digested chromosomes shows that there is a significant loss of heterochromatin between the sister chromatids at paired sites. In addition, fluorescence microscopy using anti-kinetochore serum reveals a greater inter-kinetochore distance than in controls or chromosomes digested with enzymes that spare satellite. We conclude that the presence of mouse satellite DNA in these regions is necessary for maintenance of contact between the sister chromatids of mouse mitotic chromosomes.

scholarly journals Histone Acetylation in Chromatin Containing Mouse Satellite DNA

1983 ◽  
Vol 133 (2) ◽  
pp. 379-382 ◽  
Author(s):  
Iliya G. PASHEV ◽  
Stephan I. DIMITROV ◽  
Ivan G. IVANOV ◽  
George G. MARKOV
Keyword(s):  
Histone Acetylation ◽  
Satellite Dna ◽  
Mouse Satellite Dna
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