Effect of Estrogens on Rat Serum Lipoproteins

1972 ◽  
Vol 50 (5) ◽  
pp. 474-483 ◽  
Author(s):  
P. Hill ◽  
W. G. Martin
Keyword(s):  
High Density Lipoprotein ◽  
Analytical Ultracentrifugation ◽  
Low Density Lipoprotein ◽  
Subcutaneous Administration ◽  
Density Lipoprotein ◽  
High Density ◽  
Phospholipid Content ◽  
Low Density ◽  
Rat Serum ◽  
Estradiol 17Β

Estrogens, ingredients of contraceptive drugs, were found to affect serum lipids, lipoproteins, and liver lipids of rats selectively. Subcutaneous administration of ethynylestradiol reduced the phospholipid content of the dextran sulfate soluble fraction (HDS) of the serum and decreased the rate of disappearance of the phospholipid moiety, labelled with choline-Me3H in this fraction. Estradiol-17β increased the HDS phospholipid but not its rate of turnover. On analytical ultracentrifugation of the whole serum, no low density lipoprotein was detected and the high density lipoprotein was reduced in ethynylestradiol-treated rats. Ethynylestradiol decreased the phospholipid content and turnover of lecithin in the liver. Both ethynylestradiol and estradiol-17β quantitatively increased the very low density lipoprotein, increasing the phospholipid and triglyceride contents. Data suggested that ethynylestradiol and estradiol-17β affected phospholipid synthesis in the liver to different degrees and one result of this was a change in the proportion of phospholipid in the high density lipoprotein in the rat.

Loss of A-Apoprotein Immunoreactivity during High-Density Lipoprotein Separation

1981 ◽  
Vol 18 (5) ◽  
pp. 308-313 ◽  
Author(s):  
P Johnson ◽  
R A Muirhead ◽  
T Deegan
Keyword(s):  
High Density Lipoprotein ◽  
Surface Structure ◽  
Analytical Ultracentrifugation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Low Density ◽  
Apoprotein B

By use of an electroimmunoassay, concentrations of A-apoproteins were estimated in serum and in corresponding apoprotein fractions isolated by ultracentrifugation. These values were compared with high-density lipoprotein concentrations determined by analytical ultracentrifugation. Concentrations of A-apoproteins estimated in serum were considerably higher than in isolated high-density lipoprotein fractions. These discrepancies could not be accounted for entirely by material losses into other fractions during ultracentrifugal fractionation. No comparable differences in apoprotein-B concentrations were observed during the ultracentrifugal separation of low-density lipoprotein. Concentrations of A-apoproteins estimated in the residual serum after precipitation of low-density lipoproteins by heparin and manganous ions were also lower than in the corresponding whole sera. The discrepancies persisted after treatment of serum and isolated fractions with tetramethylurea, urea (9 mol/l), and by heating at 52°C for 3 hours. It is considered that separation by ultracentrifugation induces subtle alterations in the surface structure of the lipoprotein species which give rise to changes in immunoreactivity.

scholarly journals Accumulation of High-Density Lipoprotein-Derived Estradiol-17β Fatty Acid Esters in Low-Density Lipoprotein Particles1

2001 ◽  
Vol 86 (3) ◽  
pp. 1294-1300 ◽  
Author(s):  
Hannamaarit Helisten ◽  
Anna Höckerstedt ◽  
Kristiina Wähälä ◽  
Aila Tiitinen ◽  
Herman Adlercreutz ◽  
...  
Keyword(s):  
Fatty Acid ◽  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Fatty Acid Esters ◽  
Low Density ◽  
Estradiol 17Β ◽  
Acid Esters

scholarly journals Normal high density lipoprotein inhibits three steps in the formation of mildly oxidized low density lipoprotein: steps 2 and 3

2000 ◽  
Vol 41 (9) ◽  
pp. 1495-1508 ◽  
Author(s):  
Mohamad Navab ◽  
Susan Y. Hama ◽  
G.M. Anantharamaiah ◽  
Kholood Hassan ◽  
Greg P. Hough ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein
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