Effect of L-Histidine on Human and Rat Jejunal Pyruvate Kinase Activity

1971 ◽  
Vol 49 (10) ◽  
pp. 1105-1116 ◽  
Author(s):  
Fred B. Stifel ◽  
Robert H. Herman
Keyword(s):  
Pyruvate Kinase ◽  
Inverse Relationship ◽  
Kinase Activity ◽  
Progressive Increase ◽  
Pyruvate Kinase Activity ◽  
Deficient Diet ◽  
Feeding Groups ◽  
Regulation Of Glycolysis ◽  
Complete Diet

L-Histidine was found to be an in vitro activator of both human and rat jejunal pyruvate kinase activity and rat hepatic pyruvate kinase activity. Jejunal and hepatic pyruvate kinase activities were higher in rats fed a histidine-complete diet than a histidine-deficient diet. Feeding groups of rats successively larger doses of oral histidine produced a progressive increase in rat jejunal pyruvate kinase activity. An inverse relationship was seen between the pyruvate kinase activity without histidine added and the percent stimulation with histidine added in vitro.The histidine effect appears to be a ubiquitous one in the rat, since stimulation was observed in each of the seven tissues tested. The broad range of the histidine effect suggests that it may play a fundamental role in the regulation of glycolysis in mammalian systems.

scholarly journals Lead inhibits in vitro creatine kinase and pyruvate kinase activity in brain cortex of rats

2010 ◽  
Vol 24 (3) ◽  
pp. 1045-1051 ◽  
Author(s):  
Tatiana Wannmacher Lepper ◽  
Evandro Oliveira ◽  
Gustavo Duarte Waltereith Koch ◽  
Daiane Bolzan Berlese ◽  
Luciane Rosa Feksa
Keyword(s):  
Creatine Kinase ◽  
Pyruvate Kinase ◽  
Brain Cortex ◽  
Kinase Activity ◽  
Pyruvate Kinase Activity

scholarly journals AG-348 enhances pyruvate kinase activity in red blood cells from patients with pyruvate kinase deficiency

Blood ◽  
2017 ◽  
Vol 130 (11) ◽  
pp. 1347-1356 ◽  
Author(s):  
Charles Kung ◽  
Jeff Hixon ◽  
Penelope A. Kosinski ◽  
Giovanni Cianchetta ◽  
Gavin Histen ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Pyruvate Kinase ◽  
Blood Cells ◽  
Kinase Activity ◽  
Red Cell ◽  
Pyruvate Kinase Activity ◽  
Pyruvate Kinase Deficiency ◽  
Key Points ◽  
Allosteric Activator

Key Points AG-348 is a small-molecule allosteric activator of WT red cell pyruvate kinase as well as mutant enzymes associated with hemolytic anemia. Activity in vitro, in mice, and in red blood cells suggests it may address the underlying molecular pathology in PK deficiency patients.

scholarly journals The role of mitochondria in modifying calcium-sensitive cytoplasmic metabolic activities. Modification of pyruvate kinase activity

1972 ◽  
Vol 128 (2) ◽  
pp. 415-420 ◽  
Author(s):  
J. Meli ◽  
F. L. Bygrave
Keyword(s):  
Pyruvate Kinase ◽  
Rat Liver ◽  
Kinase Activity ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Pyruvate Kinase Activity ◽  
Metabolic Activities ◽  
The One

1. The modification of pyruvate kinase activity in vitro was examined by altering the environmental [Mg2+]/[Ca2+] ratio with EDTA on the one hand and isolated rat liver mitochondria on the other. 2. Controlled additions of Ca2+ and EDTA caused pyruvate kinase activity to be alternately and rapidly switched on and off. 3. By being able to accumulate Ca2+ in preference to Mg2+ rat liver mitochondria were able to alter the [Mg2+]/[Ca2+] ratio in the vicinity of pyruvate kinase and thereby modify the activity of this enzyme. 4. The possible role of mitochondria in modifying pyruvate kinase and other ion-sensitive cytoplasmic enzyme activities is discussed.

scholarly journals Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin

1979 ◽  
Vol 180 (3) ◽  
pp. 523-531 ◽  
Author(s):  
R M Denton ◽  
N J Edgell ◽  
B J Bridges ◽  
G P Poole
Keyword(s):  
Pyruvate Kinase ◽  
Kinase Activity ◽  
Covalent Modification ◽  
Epididymal Adipose Tissue ◽  
Pyruvate Kinase Activity ◽  
Marked Diminution ◽  
Fructose 1,6 Bisphosphate ◽  
Fat Pads

1. Evidence is presented that exposure of epididymal fat-pads from fed rats to insulin leads to a marked diminution in the Km for phosphoenolpyruvate of pyruvate kinase. Effects of insulin may be readily demonstrated in experiments both in vivo and in vitro and are not secondary to the activation by the hormone of glucose transport. No effect of insulin is apparent in tissues from 48 h-starved animals. 2. The mechanism of the effect of insulin on pyruvate kinase was not established. The observed changes in Km do not appear to be the result of alterations in the amounts of bound effectors such as fructose 1,6-bisphosphate and alanine. Rather, as the effect persists in incubated extracts, it appears that a change in the degree of phosphorylation or some other covalent modification of the enzyme may be involved.

Red blood cell age, pyruvate kinase activity, and insulin receptors. Evidence that monocytes and RBCs may behave differently

Diabetes ◽  
1983 ◽  
Vol 32 (11) ◽  
pp. 1017-1022 ◽  
Author(s):  
A. Camagna ◽  
R. De Pirro ◽  
L. Tardella ◽  
L. Rossetti ◽  
R. Lauro ◽  
...  
Keyword(s):  
Blood Cell ◽  
Pyruvate Kinase ◽  
Red Blood Cell ◽  
Kinase Activity ◽  
Insulin Receptors ◽  
Pyruvate Kinase Activity ◽  
Cell Age

Red Blood Cell Age, Pyruvate Kinase Activity, and Insulin Receptors: Evidence that Monocytes and RBCs May Behave Differently

Diabetes ◽  
1983 ◽  
Vol 32 (11) ◽  
pp. 1017-1022 ◽  
Author(s):  
A. Camagna ◽  
R. D. Pirro ◽  
L. Tardella ◽  
L. Rossetti ◽  
R. Lauro ◽  
...  
Keyword(s):  
Blood Cell ◽  
Pyruvate Kinase ◽  
Red Blood Cell ◽  
Kinase Activity ◽  
Insulin Receptors ◽  
Pyruvate Kinase Activity ◽  
Cell Age

Pyruvate kinase activity in cerebral hemispheres and cerebellum-brainstem of normal and hypoxic-ischemic newborn rats

2004 ◽  
Vol 3 (3) ◽  
pp. 152-155 ◽  
Author(s):  
Hacer Yapicioglu ◽  
Mehmet Satar ◽  
Nejat Narli ◽  
Levent Kayrin ◽  
Ercan Tutak
Keyword(s):  
Pyruvate Kinase ◽  
Kinase Activity ◽  
Cerebral Hemispheres ◽  
Newborn Rats ◽  
Pyruvate Kinase Activity
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