Preparation of Methyl Derivatives of Some Organic Acids for Analysis by Gas–Liquid Chromatography

1971 ◽  
Vol 49 (8) ◽  
pp. 949-952 ◽  
Author(s):  
Craig A. Atkins ◽  
David T. Canvin
Keyword(s):  
Liquid Chromatography ◽  
Organic Acids ◽  
Thionyl Chloride ◽  
Glyoxylic Acid ◽  
Gas Liquid Chromatography ◽  
Methyl Derivative ◽  
Oxaloacetic Acid ◽  
Ethereal Diazomethane ◽  
Methyl Derivatives ◽  
Derivatives Of

One microgram to 1 mg of organic acid was completely converted to its methyl derivative when it was dissolved in methanol and treated with an excess of ethereal diazomethane. Single products were obtained from α-ketoglutaric acid, oxaloacetic acid, pyruvic acid, glyoxylic acid, or hydroxypyruvic acid on reaction with thionyl chloride – methanol. The separation of 24 organic acids by gas–liquid chromatography on a Reoplex 400 column is presented.

scholarly journals Gas-liquid chromatography of dialkyl, alkyl acyl, and diacyl derivatives of glycerol

1969 ◽  
Vol 10 (1) ◽  
pp. 128-131
Author(s):  
Randall Wood ◽  
W.J. Baumann ◽  
Fred Snyder ◽  
H.K. Mangold
Keyword(s):  
Liquid Chromatography ◽  
Gas Liquid Chromatography ◽  
Derivatives Of

Screening for urinary oligosaccharides: a quantitative procedure.

1978 ◽  
Vol 24 (4) ◽  
pp. 669-674 ◽  
Author(s):  
P Purkiss ◽  
R C Hughes ◽  
R W Watts
Keyword(s):  
Liquid Chromatography ◽  
Age Groups ◽  
Normal Subjects ◽  
Gel Chromatography ◽  
Gas Liquid Chromatography ◽  
Complex Carbohydrates ◽  
Derivatives Of ◽  
Quantitative Procedure ◽  
Urinary Oligosaccharides

Abstract A method for screening urine for abnormalities in glycoconjugate excretion is presented. An oligosaccharide-containing fraction is isolated from urine by gel chromatography and the carbohydrate-containing components in this fraction are degraded to monosaccharides, suitable derivatives of which are analyzed by gas-liquid chromatography. A pattern which characterizes the monosaccharides originating in complex carbohydrates in urine is thus obtained. Data obtained by applying this method to normal subjects in various age groups are presented and the applicability of the method and its limitations are illustrated by the use of examples from patients with previously diagnosed disorders of glycoconjugate metabolism.

Fast Cleanup of Difficult Substrates for Determination of Fenitrothion and Some Derivatives

1981 ◽  
Vol 64 (6) ◽  
pp. 1470-1473
Author(s):  
Patrick Haché ◽  
René Marquette ◽  
Gilles Volpé ◽  
Victorin N Mallet
Keyword(s):  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Parent Compound ◽  
Chicken Liver ◽  
Gas Liquid Chromatography ◽  
Fast Recovery ◽  
Simple Method ◽  
Flame Photometric Detector ◽  
Derivatives Of

Abstract A simple method is described for the fast recovery of fenitrothion, an organophosphorus insecticide, from soil, chicken liver, urine, clams, and pine needles. The substrate is homogenized with acetonitrile or methanol, diluted with water, and passed through a column containing Amberlite XAD-7. Fenitrothion is recovered quantitatively by eluting with 4 portions of 25 mL ethyl acetate. After evaporation, the compound is determined quantitatively by gas-liquid chromatography with a flame photometric detector. The procedure is also suitable for some derivatives of fenitrothion, namely, fenitrooxon and S-methylfenitrothion. As low as 0.05 ppm of the parent compound may be determined.

Sign in / Sign up

Export Citation Format

Share Document