Studies on Spermatogenesis in Rats. III. Effects of Hormonal Treatment on Differentiation Kinetics of the Spermatogenic Cycle in Regressed Hypophysectomized Rats

1971 ◽  
Vol 49 (7) ◽  
pp. 768-775 ◽  
Author(s):  
V. L. W. Go ◽  
R. G. Vernon ◽  
I. B. Fritz
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Hormonal Treatment ◽  
Testicular Function ◽  
Carnitine Acetyltransferase ◽  
Hormonal Replacement ◽  
Hypophysectomized Rats ◽  
Kinetics Of ◽  
Stimulating Hormone

The general hormonal requirements for the restoration of spermatogenesis in regressed hypophysectomized rats were investigated. With the aid of the Staput fractionation technique, it was established that thymidine-3H was readily incorporated into spermatogonia and resting spermatocytes. Labeled cells did not progress to form appreciable numbers of primary spermatocytes or spermatids in the absence of hormonal replacement. The inhibition of formation of pachytene primary spermatocytes in hypophysectomized rats was overcome by administration of follicle-stimulating hormone (FSH), luteinizing hormone (LH), or testosterone, but a combination of either FSH plus LH, or FSH plus testosterone, was required for the progression of pachytene primary spermatocytes to spermatids and spermatozoa. Carnitine acetyltransferase (CAT) measurements in testes from various groups of animals provided ancillary evidence consistent with the conclusion that either FSH, LH, or testosterone was required for the normal restoration of pachytene-diplotene spermatocyte formation. However, one or more additional blocks in spermatogenesis existed in hypophysectomized animals, since elevation of depressed testicular CAT levels in hypophysectomized rats to normal levels required FSH plus LH, or FSH plus testosterone. Cortisone and thyroxin treatment had no measurable effects on testicular function in hypophysectomized rats.

Endocrine and local testicular regulation

2011 ◽  
pp. 1347-1353
Author(s):  
Ilpo Huhtaniemi
Keyword(s):  
Growth Factors ◽  
Luteinizing Hormone ◽  
Leydig Cell ◽  
Follicle Stimulating Hormone ◽  
Regulatory System ◽  
The Other ◽  
Seminiferous Tubules ◽  
Testicular Function ◽  
Cell Product ◽  
Stimulating Hormone

The testis has two functions, androgen production and spermatogenesis, and a key role in their regulation is played by the two pituitary gonadotropins, luteinizing hormone and follicle-stimulating hormone (FSH). Other hormones and growth factors also influence testicular function, often by modulating the gonadotropin effects. Moreover, a plethora of local paracrine and autocrine signals within the testis are known. The main testicular hormone, testosterone, a Leydig cell product, regulates spermatogenesis in seminiferous tubules in paracrine fashion. The other functions of testosterone are endocrine, occurring outside the testis. This chapter summarizes the main hormonal regulatory system of the testis, the hypothalamic–pituitary–testicular axis, and how its effects are modulated by other extratesticular hormones and local testicular factors.

EFFECTS OF FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE ON PLASMA TESTOSTERONE LEVELS IN HYPOPHYSECTOMIZED AND IN INTACT IMMATURE AND ADULT MALE RATS

1974 ◽  
Vol 61 (2) ◽  
pp. 193-198 ◽  
Author(s):  
S. EL SAFOURY ◽  
A. BARTKE
Keyword(s):  
Luteinizing Hormone ◽  
Adult Male ◽  
Follicle Stimulating Hormone ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Adult Rats ◽  
Testosterone Levels ◽  
Hypophysectomized Rats ◽  
Stimulating Hormone

SUMMARY The effects of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on plasma testosterone levels were examined in hypophysectomized and in intact immature and adult male rats. The animals were injected with saline, LH, FSH, or both gonadotrophins twice daily for 3·5 days and were killed 3 h after the last injection. Plasma testosterone levels were measured by radioimmunoassay. In immature hypophysectomized rats, plasma testosterone levels were not changed by treatment with LH, FSH or LH plus FSH. The weight of the testes and of the seminal vesicles was increased only in animals injected with both LH and FSH. In adult hypophysectomized rats, LH caused the expected increase in plasma testosterone levels, while FSH injected alone had no effect. Plasma testosterone levels in rats treated with 5 μg LH and 20 μg FSH were significantly greater than those in animals given 5 μg LH alone. However, the same dose of FSH did not potentiate the action of 25 μg LH on plasma testosterone levels. In adult hypophysectomized rats the weight of testes was not affected by any of the treatments. The weight of the seminal vesicles was increased by the higher dose of LH and addition of FSH caused no further increase. In intact immature and adult rats plasma testosterone levels and the weight of testes were not changed by any of the treatments. Seminal vesicle weight was increased only in adult rats treated with the higher dose of LH together with FSH. The results demonstrate that FSH potentiates the action of low doses of LH on plasma testosterone levels in adult hypophysectomized rats and suggest that FSH may be involved in the regulation of androgen secretion by the rat testis.

THE VENTRAL PROSTATE WEIGHT METHOD IN HYPOPHYSECTOMIZED RATS FOR LUTEINIZING HORMONE

1968 ◽  
Vol 59 (4) ◽  
pp. 622-628 ◽  
Author(s):  
Peter Christiansen
Keyword(s):  
Luteinizing Hormone ◽  
Urine Sample ◽  
Mammary Carcinoma ◽  
Positive Response ◽  
Follicle Stimulating Hormone ◽  
Ventral Prostate ◽  
Prostate Weight ◽  
Hypophysectomized Rats ◽  
Weight Method ◽  
Stimulating Hormone

ABSTRACT The influence of ovine follicle stimulating hormone (NIH-FSH-S-3) on ovine luteinizing hormone (NIH-LH-S-8) in the Ventral Prostate Weight method (VPW) was studied. Adding of NIH-FSH-S-3 to NIH-LH-S-8 at ratios of 1:1, 4:1 and 10:1 gave no significantly higher responses than did NIH-LH-S-8 alone. Urinary extracts from 2 women, hypophysectomized for metastasizing mammary carcinoma and from 3 children between 2 and 5 years old (1 boy, 2 girls) gave no positive response with the doses employed (1/4 of a 24 hours urine sample total per rat). It is concluded that the Ventral Prostate Weight method in hypophysectomized rats is specific for the assay of luteinizing hormone.

EFFECT OF OVINE LUTEINIZING HORMONE ON THE SECRETION OF PROGESTERONE IN VITRO BY OVARIAN FOLLICLES FROM HYPOPHYSECTOMIZED RATS TREATED WITH FOLLICLE-STIMULATING HORMONE

1978 ◽  
Vol 77 (3) ◽  
pp. 419-420
Author(s):  
A. R. LABARBERA ◽  
MERO R. NOCENTI
Keyword(s):  
New York ◽  
Luteinizing Hormone ◽  
Corn Oil ◽  
Follicle Stimulating Hormone ◽  
Virgin Female ◽  
Ovarian Follicles ◽  
Female Rats ◽  
Hypophysectomized Rats ◽  
Stimulating Hormone

Department of Physiology, College of Physicians and Surgeons, Columbia University, New York 10032, U.S.A. (Received 14 November 1977) Ovarian follicles from oestrous, pro-oestrous and hypophysectomized rats have the capacity to secrete progestins in vitro and to respond to luteinizing hormone (LH) by increasing this secretion (Stoklosowa & Nalbandov, 1972; LaBarbera, Nocenti & Castellano, 1974; Lindner, Tsafriri, Lieberman, Zor, Koch, Bauminger & Barnea, 1974). Follicles from hypophysectomized rats, untreated or treated with follicle-stimulating hormone (FSH), were therefore studied in experiments in vitro to investigate further the gonadotrophic control of progesterone secretion. Mature virgin female rats (200–250 g) were hypophysectomized and, beginning on day 4 after the operation, received a total of 360 μg ovine FSH (NIH-FSH-S9)/100 g body weight, injected s.c. in corn oil as six divided doses, one every 12 h for 3 days. Untreated controls received corn oil only. On day 7 after hypophysectomy, the ovaries were excised and

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