The regulation of protein synthesis in nephrotic rat liver ribosome preparations

1970 ◽  
Vol 48 (11) ◽  
pp. 1222-1227 ◽  
Author(s):  
D. M. Nicholls ◽  
G. R. Girgis
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Ribonuclease Activity ◽  
Liver Homogenates

The incorporation of 14C-phenylalanine into peptide was measured in microsome and ribosome preparations of control and nephrotic rat liver homogenates. Microsomes and ribosomes were incubated with liver postmicrosomal supernatant (S105) and 14C-phenylalanine. Ribosomes were incubated with a control liver transferase preparation and 14C-phenylalanyl-tRNA. In the nephrotic preparations the incorporation of 14C-phenylalanine was greater than it was in the control preparations either with endogenous mRNA or poly U. Since ribonuclease activity did not differ in control and nephrotic preparations and since using bentonite to inhibit ribonuclease did not change the nephrotic effect, the increased incorporation could not be attributed to decreased ribonuclease activity.

scholarly journals Ribonuclease Activity of Rat Liver Perchloric Acid-soluble Protein, a Potent Inhibitor of Protein Synthesis

1999 ◽  
Vol 274 (29) ◽  
pp. 20688-20692 ◽  
Author(s):  
Ryo Morishita ◽  
Akihito Kawagoshi ◽  
Tatsuya Sawasaki ◽  
Kairat Madin ◽  
Tomio Ogasawara ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Perchloric Acid ◽  
Soluble Protein ◽  
Potent Inhibitor ◽  
Protein A ◽  
Ribonuclease Activity ◽  
Inhibitor Of Protein Synthesis

scholarly journals THE INCORPORATION OF THE CARBOXYL CARBON FROM ACETATE INTO CHOLESTEROL BY RAT LIVER HOMOGENATES

1954 ◽  
Vol 206 (1) ◽  
pp. 471-481 ◽  
Author(s):  
Ivan D. Frantz ◽  
Nancy L.R. Bucher ◽  
Henny S. Schneider ◽  
Naomi H. McGovern ◽  
Ruth Kingston
Keyword(s):  
Rat Liver ◽  
Liver Homogenates ◽  
Carboxyl Carbon

scholarly journals Postnatal development of rat liver mitochondrial functions. The roles of protein synthesis and of adenine nucleotides.

1988 ◽  
Vol 263 (16) ◽  
pp. 7767-7775
Author(s):  
C Valcarce ◽  
R M Navarrete ◽  
P Encabo ◽  
E Loeches ◽  
J Satrústegui ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Postnatal Development ◽  
Rat Liver ◽  
Adenine Nucleotides ◽  
Mitochondrial Functions

scholarly journals STIMULATION BY DINITROPHENOL OF FORMATION OF MELANIN-LIKE SUBSTANCE FROM TYROSINE BY RAT LIVER HOMOGENATES

1957 ◽  
Vol 225 (2) ◽  
pp. 735-744
Author(s):  
Henry Kamin ◽  
Mildred A. Koon ◽  
Philip Handler
Keyword(s):  
Rat Liver ◽  
Liver Homogenates

Glucocorticoid effects on respiration and metabolism by rat liver homogenates

1962 ◽  
Vol 202 (2) ◽  
pp. 343-346 ◽  
Author(s):  
Dennis D. Goetsch ◽  
L. E. McDonald
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Rat Liver ◽  
Inorganic Phosphate ◽  
Chronic Administration ◽  
Glucocorticoid Treatment ◽  
Carbohydrate Utilization ◽  
Protein Levels ◽  
Liver Homogenates ◽  
And Control

The effects of glucocorticoid administration on oxygen uptake, glucose and glycogen disappearance, lactic acid formation, and inorganic phosphate and protein levels in rat liver homogenates have been studied. A single injection of hydrocortisone, prednisolone, or 9 α-fluoroprednisolone 5 hr before sacrifice resulted in a highly significant increase in oxygen uptake by rat liver homogenates, whereas chronic administration of prednisolone daily for 7 days caused a marked inhibition in homogenate respiration. Glycolytic rate did not appear to be affected by single injections since endogenous carbohydrate utilization was similar in liver homogenates prepared from control and treated animals. Incubation of liver homogenates under aerobic conditions disclosed that inorganic phosphate levels were decreased in homogenates from corticoid-treated rats, whereas these levels were similar in treated and control liver homogenates incubated under nitrogen. Under anaerobic conditions, liver homogenates from treated rats accumulated lactic acid more rapidly than untreated liver homogenates. Glucocorticoid treatment did not appear to affect protein disappearance since no differences between protein levels in treated and untreated rat liver homogenates were detected following incubation.

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