Agents affecting lipid metabolism. XXXIX. Effect of combined administration of ethyl chlorophenoxyisobutyrate and cholestyramine on cholesterol biosynthesis in the rat

1970 ◽  
Vol 48 (9) ◽  
pp. 1022-1023 ◽  
Author(s):  
M. N. Cayen ◽  
D. Dvornik
Keyword(s):  
Lipid Metabolism ◽  
Cholesterol Synthesis ◽  
Cholesterol Biosynthesis ◽  
Combined Effect ◽  
Hepatic Cholesterol ◽  
Hepatic Cholesterol Synthesis ◽  
Combined Administration ◽  
Liver Homogenates

Rats were fed ethyl p-chlorophenoxyisobutyrate (CPIB) and cholestyramine, alone and in combination. Regarding levels of circulating cholesterol, cholestyramine had no effect, while a fall was observed with CPIB given alone or in combination with cholestyramine. Subsequently, the combined effect of both agents was elicited by measuring the incorporation of 2-14C-acetate into cholesterol by liver homogenates of treated rats; addition of CPIB decreased the cholestyramine-induced increase in the rate of hepatic cholesterol synthesis.

Agents affecting lipid metabolism. XXXI. Properties of an inhibitor of cholesterol synthesis present in rabbit liver mitochondria

1968 ◽  
Vol 46 (2) ◽  
pp. 179-187 ◽  
Author(s):  
M. N. Cayen ◽  
D. Dvornik
Keyword(s):  
Cholesterol Synthesis ◽  
Liver Mitochondria ◽  
Aqueous Extracts ◽  
Hepatic Cholesterol ◽  
Large Molecules ◽  
Hepatic Cholesterol Synthesis ◽  
Significant Enrichment ◽  
Liver Homogenates ◽  
Mitochondrial Extract

Aqueous extracts of mitochondria from livers of starved animals have been reported to inhibit hepatic cholesterol synthesis. Some properties of a mitochondrial extract prepared from starved rabbits have been studied. The extract depressed the incorporation of both 2-14C-acetate and 3H-mevalonate into cholesterol by rat liver homogenates, giving concentration–dependent responses. The active factor was unstable to heat, but stable under in vitro incubation conditions, to pH changes, and to storage in the dark and cold. It was cationic and associated with one or more large molecules (mol. wt. 50 000–150 000); it was not cleaved by trypsin digestion. Fractionation experiments did not result in any significant enrichment of inhibitory activity.

Regulation of hepatic cholesterol synthesis by a novel protein (SPF) that accelerates cholesterol biosynthesis

2006 ◽  
Vol 20 (14) ◽  
pp. 2642-2644 ◽  
Author(s):  
Norihito Shibata ◽  
Kou‐ichi Jishage ◽  
Makoto Arita ◽  
Miho Watanabe ◽  
Yosuke Kawase ◽  
...  
Keyword(s):  
Cholesterol Synthesis ◽  
Cholesterol Biosynthesis ◽  
Hepatic Cholesterol ◽  
Hepatic Cholesterol Synthesis ◽  
Novel Protein

From GWAS to GEMMs: Sestrin1 and cholesterol biosynthesis

2020 ◽  
Vol 12 (539) ◽  
pp. eabb5669
Author(s):  
Emily J. Gallagher
Keyword(s):  
Cholesterol Synthesis ◽  
Cholesterol Biosynthesis ◽  
Hepatic Cholesterol ◽  
Hepatic Cholesterol Synthesis

Sestrin1 regulates hepatic cholesterol synthesis and circulating lipid concentrations.

AN INCREASED RATE OF CHOLESTEROL BIOSYNTHESIS WITH MICROSOME FRACTIONS OF LIVER FROM KETOTIC GUINEA PIGS

1962 ◽  
Vol 40 (1) ◽  
pp. 1749-1762 ◽  
Author(s):  
F. Sauer
Keyword(s):  
Young Adult ◽  
Guinea Pigs ◽  
Cholesterol Synthesis ◽  
Cholesterol Biosynthesis ◽  
Maximum Activity ◽  
Sterol Synthesis ◽  
Differential Centrifugation ◽  
Microsome Fraction ◽  
Liver Homogenates ◽  
Increased Activity

Cholesterol synthesis was studied in liver fractions obtained by differential centrifugation from young, adult, and ketotic guinea pigs. Both 10,000 × g and 105,000 × g sediment was required for maximum activity. Incubations were carried out in the presence of appropriate liver fractions from young guinea pigs in order to overcome the low rates of cholesterol synthesis in liver homogenates from adult guinea pigs. Microsome fractions from ketotic hyperlipemic guinea pigs actively promoted sterol synthesis when incubated with mitochondria plus supernatant from young guinea pigs, while microsome fractions from adult controls (fed or starved) decreased the rate of sterol synthesis in the same incubation system. The results of this investigation indicate that microsomes from hyperlipemic ketotic guinea pigs do not have a block in cholesterol synthesis characteristic of microsomes from starved animals, and that this microsome fraction has increased activity of HMG-CoA2reductase, one of the key enzymes of cholesterol synthesis.

Increased hepatic cholesterol synthesis in normal rats by cross-circulation with ileal bypassed partners

1979 ◽  
Vol 34 (4) ◽  
pp. 383-389 ◽  
Author(s):  
Philip D. Schneider ◽  
Ignacio J. Guzman ◽  
Richard D. Rucker ◽  
Thomas G. Stocks ◽  
Richard L. Varco ◽  
...  
Keyword(s):  
Cholesterol Synthesis ◽  
Hepatic Cholesterol ◽  
Hepatic Cholesterol Synthesis

Selective effects of two novel squalene synthase inhibitors on hepatic cholesterol synthesis in the rat

1994 ◽  
Vol 109 (1-2) ◽  
pp. 252 ◽  
Author(s):  
G.J. Smith ◽  
R.G. Davidson ◽  
C. Dunkley ◽  
G.R. Brown ◽  
K.B. Mallion ◽  
...  
Keyword(s):  
Cholesterol Synthesis ◽  
Squalene Synthase ◽  
Hepatic Cholesterol ◽  
Hepatic Cholesterol Synthesis ◽  
Selective Effects

Hepatic cholesterol synthesis and lipoprotein levels impaired by dietary fructose and saturated fatty acids in mice: Insight on PCSK9 and CD36

Nutrition ◽  
2020 ◽  
Vol 79-80 ◽  
pp. 110954
Author(s):  
Reyhan Nergiz-Unal ◽  
Elif Ulug ◽  
Betul Kisioglu ◽  
Funda Tamer ◽  
Mahmut Bodur ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cholesterol Synthesis ◽  
Saturated Fatty Acids ◽  
Hepatic Cholesterol ◽  
Hepatic Cholesterol Synthesis ◽  
Dietary Fructose
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