Inhibition of glycolysis in Ehrlich ascites tumor cells incubated with formimino-L-glutamate

1969 ◽  
Vol 47 (4) ◽  
pp. 419-422 ◽  
Author(s):  
Lydia J. Fontenelle ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
De Novo ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Phosphoribosyl Pyrophosphate ◽  
Triose Phosphate ◽  
Glucose Inhibition ◽  
Ehrlich Ascites ◽  
Inhibition Of Glycolysis

Incubation of tumor cells with formiminoglutamate leads to inhibition of lactate synthesis from glucose, inhibition of phosphoribosyl pyrophosphate synthesis, and inhibition of purine ribonucleotide synthesis de novo and from purine bases. Lactate synthesis from inosine was increased. These effects may result from inhibition of triose phosphate isomerase by a metabolite of formiminoglutamate.

Effects of Actinomycin D on Purine Ribonucleotide Metabolism in Ehrlich Ascites Tumor Cells In Vitro

1974 ◽  
Vol 52 (3) ◽  
pp. 263-267 ◽  
Author(s):  
Floyd F. Snyder ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
Actinomycin D ◽  
De Novo ◽  
Acid Synthesis ◽  
Purine Metabolism ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

Actinomycin D treatment of Ehrlich ascites tumor cells in vitro causes slight to moderate inhibition of purine ribonucleotide synthesis de novo and from purine bases, and strong inhibition of inosinate dehydrogenase activity. These effects have the same dose–response relationship as inhibition of RNA synthesis by this drug. Daunomycin has similar effects on purine metabolism at a concentration that substantially inhibits nucleic acid synthesis. Actinomycin D treatment leads to elevated intracellular concentrations of ATP and GTP, and the effects of this drug on purine metabolism are believed to be mediated by these purine ribonucleoside triphosphates.

Role of orthophosphate concentration in the regulation of ribose phosphate synthesis and purine metabolism in Ehrlich ascites tumor cells

1977 ◽  
Vol 55 (8) ◽  
pp. 834-840 ◽  
Author(s):  
Jerzy Barankiewicz ◽  
Mary L. Battell ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
Purine Metabolism ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Phosphoribosyl Pyrophosphate ◽  
Ribose Phosphate ◽  
Ehrlich Ascites ◽  
In Cells

Concentrations of intracellular orthophosphate were determined in Ehrlich ascites tumor cells incubated with glucose, inosine, or uridine in media of different orthophosphate concentration. The effects of orthophosphate concentration on the accumulation of lactate and of phosphoribosyl pyrophosphate and on concentrations of ribose 1-phosphate and ribose 5-phosphate in tumor cells incubated with glucose were also determined. Both the phosphorolysis of inosine and the rate of catabolism of ATP in cells incubated with 2-deoxyglucose were also influenced by the orthophosphate concentration of the medium.

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