Agents affecting lipid metabolism. XXXVI. Effect of N-cyclohexyl linoleamide on hepatic cholesterogenesis in vitro

1968 ◽  
Vol 46 (11) ◽  
pp. 1430-1432 ◽  
Author(s):  
D. Dvornik
Keyword(s):  
Lipid Metabolism ◽  
Rat Liver ◽  
Final Concentration ◽  
Normal Rat ◽  
Liver Homogenates ◽  
Hepatic Cholesterogenesis

N-Cyclohexyl linoleamide, reported to be antihypercholesterolemic in rabbits, was examined for its effect on hepatic cholesterogenesis in vitro. At a final concentration of 1 × 10−3 M the compound had no effect on the incorporation into cholesterol of 3H-acetate and 2-14C-mevalonate incubated simultaneously with normal rat liver homogenates.

Substrate specificity of iodothyronine 5′-deiodinase in rat liver homogenates and its requirements of divalent cations in vitro

Life Sciences ◽  
1986 ◽  
Vol 38 (24) ◽  
pp. 2231-2238 ◽  
Author(s):  
Shinya Kobayshi ◽  
Yan Gao ◽  
Richard L. Ong ◽  
Constance S. Pittman
Keyword(s):  
Substrate Specificity ◽  
Rat Liver ◽  
Divalent Cations ◽  
Liver Homogenates

Effect of Aureomycin on the Respiration of Normal Rat Liver Homogenates

Science ◽  
1951 ◽  
Vol 113 (2932) ◽  
pp. 273-273
Author(s):  
J. C. Van Meter ◽  
J. J. Oleson
Keyword(s):  
Rat Liver ◽  
Normal Rat ◽  
Liver Homogenates

Metabolism in vitro of n-methylamphetamine with rat liver homogenates

1977 ◽  
Vol 26 (11) ◽  
pp. 1043-1049 ◽  
Author(s):  
Ronald T. Coutts ◽  
Susan H. Kovach
Keyword(s):  
Rat Liver ◽  
Liver Homogenates

THE ROLE OF THE ADRENAL GLAND IN THE CONTROL OF AMINO ACID INCORPORATION INTO PROTEIN OF ISOLATED RAT LIVER MICROSOMES

1960 ◽  
Vol 21 (2) ◽  
pp. 177-189 ◽  
Author(s):  
A. KORNER
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Female Rats ◽  
Male Rats ◽  
Rat Liver Microsomes ◽  
Hypophysectomized Rats ◽  
Normal Rat ◽  
Secondary Result

SUMMARY 1. Microsomes, isolated from rat liver a day after adrenalectomy, incorporate more radioactive amino acid into their protein in vitro than microsomes from normal rat liver. This enhanced rate of incorporation progressively declines with time after adrenalectomy until it reaches a plateau level which is below the normal rate of incorporation. 2. Following adrenalectomy microsomes isolated from liver of male rats show a greater rise in incorporating ability than those from liver of female rats, and maintain it longer. 3. Most of the increased incorporation observed in the in vitro system soon after adrenalectomy of the rat, and most of the decreased incorporation observed in rats adrenalectomized for some time, results from alterations in the microsomes which change their ability to incorporate activated amino acids into proteins. 4. Treatment of rats with cortisol acetate results in an increase in the ability of liver microsomes to incorporate amino acid into protein. This heightened incorporating ability is probably a secondary result of the breakdown of extrahepatic tissue protein which is stimulated by cortisol. 5. Somewhat similar responses to acute adrenalectomy and to treatment with cortisol were found in hypophysectomized rats. 6. The protein anabolic response of adrenalectomized rats to treatment with insulin, and of adrenalectomized-hypophysectomized rats to treatment with insulin or growth hormone, is greater than that shown by rats which possess adrenal glands.

INHIBITION OF CHOLESTEROL FORMATION BY RAT LIVER HOMOGENATES

1955 ◽  
Vol 33 (2) ◽  
pp. 135-138 ◽  
Author(s):  
B. B. Migicovsky
Keyword(s):  
Vitamin A ◽  
Rat Liver ◽  
Cholesterol Synthesis ◽  
Liver Homogenate ◽  
Inhibitory Factor ◽  
Normal Rat ◽  
Liver Homogenates

The inability of liver homogenates, from starved and vitamin A deficient rats, to synthesize cholesterol is illustrated. A possible reason for this phenomenon is that these preparations inhibit cholesterol synthesis when added to a liver homogenate from a normal rat. The inhibitory factor or factors are present in both the supernate and residue portions of the homogenate, although the residue matter is more inhibitory.

scholarly journals Aqueous Extracts of Teucrium polium Possess Remarkable Antioxidant Activity In Vitro

2006 ◽  
Vol 3 (3) ◽  
pp. 329-338 ◽  
Author(s):  
Predrag Ljubuncic ◽  
Suha Dakwar ◽  
Irina Portnaya ◽  
Uri Cogan ◽  
Hassan Azaizeh ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Rat Liver ◽  
Antioxidant Properties ◽  
Liver Homogenate ◽  
Plasma Oxidation ◽  
Teucrium Polium ◽  
Liver Homogenates ◽  
Β Carotene

Teucrium poliumL. (Lamiaceae) (RDC 1117) is a medicinal plant whose species have been used for over 2000 years in traditional medicine due to its diuretic, diaphoretic, tonic, antipyretic, antispasmodic and cholagogic properties. The therapeutic benefit of medicinal plants is often attributed to their antioxidant properties. We previously reported that an aqueous extract of the leaves and stems of this plant could inhibit iron-induced lipid peroxidation in rat liver homogenate at concentrations that were not toxic to cultured hepatic cells. Others have reported that organic extracts of the aerial components of this plant could inhibit oxidative processes. Against this background, we felt further investigation on the antioxidant action of the extract ofT. poliumprepared according to traditional Arab medicine was warranted. Accordingly, we assessed (i) its ability to inhibit (a) oxidation of β-carotene, (b) 2,2′-azobis(2-amidinopropan) dihydrochloride (AAPH)-induced plasma oxidation and (c) iron-induced lipid peroxidation in rat liver homogenates; (ii) to scavenge the superoxide ($${\hbox{ O }}_{2}^{\bullet -}$$) radical and the hydroxyl radical (OH•); (iii) its effects on the enzyme xanthine oxidase activity; (iv) its capacity to bind iron; and (v) its effect on cell glutathione (GSH) homeostasis in cultured Hep G2 cells. We found that the extract (i) inhibited (a) oxidation of β-carotene, (b) AAPH-induced plasma oxidation (c) Fe2+-induced lipid peroxidation in rat liver homogenates (IC50 = 7 ± 2 μg ml−1); (ii) scavenged $${\hbox{ O }}_{2}^{\bullet -}$$(IC50 = 12 ± 3 μg ml−1) and OH• (IC50 = 66 ± 20 μg ml−1); (iii) binds iron (IC50 = 79 ± 17 μg ml−1); and (iv) tended to increase intracellular GSH levels resulting in a decrease in the GSSG/GSH ratio. These results demonstrate that the extract prepared from theT. poliumpossesses antioxidant activityin vitro. Further investigations are needed to verify whether this antioxidant effect occursin vivo.

Diminished hepatic triiodothyronine production in Gunn rats

1986 ◽  
Vol 113 (2) ◽  
pp. 281-288 ◽  
Author(s):  
J. R. Saltzman ◽  
D. W. Clark ◽  
R. D. Utiger
Keyword(s):  
Thyroid Hormone ◽  
Rat Liver ◽  
Liver Homogenate ◽  
Unconjugated Bilirubin ◽  
Thyroid Hormone Metabolism ◽  
Gunn Rats ◽  
Deiodinase Activity ◽  
Sediment Fraction ◽  
Liver Homogenates

Abstract. The liver is a major site of conversion of thyroxine (T4) to the more active thyroid hormone 3,5,3'-triiodothyronine (T3). Hepatic T4 to T3 conversion is altered by a variety of pathological processes and pharmacological agents. We studied T4 to T3 conversion in glucuronyl transferase deficient homozygous Gunn rats because they have a hepatic enzyme abnormality which leads to hyperbilirubinaemia, and also because they have been reported to have alterations in thyroid hormone metabolism. An in vitro incubation system employing the 10 000 × g supernatant of liver homogenate was used, and T3 production was measured by radioimmunoassay. Experiments were done using substrate concentrations ranging from 0.56 to 20 μm, tissue protein in concentrations ranging from 0.625 to 20 mg and incubation times of 15 to 60 min. T3 production by liver homogenates from homozygous Gunn rats in these studies ranged from 29 to 70% of that produced by liver homogenates from phenotypically normal heterozygous Gunn rats. The deficit in hepatic T3 production by homozygous rats could not be overcome by increasing cofactor concentrations. After ultracentrifugation at 100 000 μ g, T4-5'-deiodinase activity was found primarily in the 100 000 × g sediment fraction. Homogygous rat liver 100 000 × g sediment T3 production was 55% of that of the heterozygous rat liver 100 000 × g sediment. Liver cytosol from both homozygous and heterozygous rats inhibited microsomal T4-5'-deiodinase activity similarly. Addition of unconjugated bilirubin to liver homogenates resulted in reduction of T3 production in livers from both homozygous and heterozygous rats. Thus the diminished capacity for hepatic conversion of T4 to T3 in homozygous Gunn rats may be due to inhibition of T4-5'-deiodinase activity by high endogenous levels of unconjugated bilirubin.

Sign in / Sign up

Export Citation Format

Share Document