Protein–carbohydrate interaction. XV. The role of bivalent cations in concanavalin A – polysaccharide interaction

1968 ◽  
Vol 46 (9) ◽  
pp. 1147-1150 ◽  
Author(s):  
B. B. L. Agrawal ◽  
I. J. Goldstein
Keyword(s):  
Acetic Acid ◽  
Protein Structure ◽  
Concanavalin A ◽  
Optimum Range ◽  
Metal Free ◽  
Cation Concentration ◽  
Bivalent Cations ◽  
High Concentrations

Extensive dialysis of concanavalin A solution against 1.0 M acetic acid results in the loss of its ability to precipitate specific polysaccharides. This activity is restored upon the addition of bivalent cations such as Mg2+, Mn2+, Ca2+, Co2+, and Zn2+. An optimum range of the cation concentration is observed for the activation of metal-free concanavalin A. High concentrations of bivalent cations (> 0.05 M) are inhibitory to the interaction of concanavalin A with specific polysaccharides. Of all the metals tested, Mn2+ appears to be bound most strongly. Urea and guanidine-HCl cause the inhibition of polysaccharide-precipitating activity of concanavalin A, probably by virtue of their unfolding effect on the protein structure.

Polarity of regeneration in excised leaves of Crassula argentea. I. A role of auxin

1983 ◽  
Vol 61 (4) ◽  
pp. 1058-1063 ◽  
Author(s):  
Karol E. Paterson
Keyword(s):  
Acetic Acid ◽  
Wound Response ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Developmental Sequence ◽  
Agar Culture ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

High concentrations of four auxins, naphthalene acetic acid, indole 3-acetic acid, 2,4-dichlorophenoxyacetic acid, and indole 3-butyric acid, added to leaf segments of Crassula argentea in agar culture eliminate the strong polarity of the regeneration by inducing the formation of distal plantlets. The auxins also changed the normal wound response by inducing the formation of callus on the cut surfaces. All of the auxins increased the numbers of roots formed and inhibited the number of shoots. Unlike cytokinin, which had no effect on the polarity but altered the developmental sequence of regeneration, none of the auxins had any effect on the normal developmental sequence of regeneration, but did affect the morphology of the newly formed roots. There were differential concentration responses for each of the four auxins.

Role of SOD in the protection of Rhizophora mangle on gastric injury induced by ethanol, ischaemia-reperfusion and acetic acid in rats

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
FM de-Faria ◽  
A Luiz-Ferreira ◽  
ACA Almeida ◽  
V Barbastefano ◽  
MA Silva ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Rhizophora Mangle ◽  
Gastric Injury ◽  
Ischaemia Reperfusion

The role of microbiota in concanavalin A-mediated liver injury

2018 ◽  
Vol 56 (01) ◽  
pp. E2-E89
Author(s):  
B Schiller ◽  
C Wegscheid ◽  
L Berkhout ◽  
A Zarzycka ◽  
U Steinhoff ◽  
...  
Keyword(s):  
Liver Injury ◽  
Concanavalin A

The role of acetic acid in FeCO3 scale deposition on CO2 corrosion of API X65 carbon steel under high temperatures

2021 ◽  
pp. 1-12
Author(s):  
Bernardo Augusto Farah Santos ◽  
Rhuan Costa Souza ◽  
Maria Eduarda Dias Serenario ◽  
Eugenio Pena Mendes Junior ◽  
Thiago Araujo Simões ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Carbon Steel ◽  
High Temperatures ◽  
Co2 Corrosion ◽  
Scale Deposition

Role of Indole-3-Acetic Acid in NAFLD Amelioration After Sleeve Gastrectomy

2021 ◽  
Author(s):  
Yu Wang ◽  
Geng Wang ◽  
Jie Bai ◽  
Ning Zhao ◽  
Qingbo Wang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Sleeve Gastrectomy ◽  
Indole 3 Acetic Acid

scholarly journals TP53-Induced Glycolysis and Apoptosis Regulator (TIGAR) Is Upregulated in Lymphocytes Stimulated with Concanavalin A

2021 ◽  
Vol 22 (14) ◽  
pp. 7436
Author(s):  
Helga Simon-Molas ◽  
Xavier Vallvé-Martínez ◽  
Irene Caldera-Quevedo ◽  
Pere Fontova ◽  
Claudia Arnedo-Pac ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Carbon Flux ◽  
Human Lymphocytes ◽  
Tumor Development ◽  
Pentose Phosphate ◽  
Akt Signaling Pathway ◽  
G6pdh Activity ◽  
Physiological Conditions ◽  
Glucose 6 Phosphate Dehydrogenase

The glycolytic modulator TP53-Inducible Glycolysis and Apoptosis Regulator (TIGAR) is overexpressed in several types of cancer and has a role in metabolic rewiring during tumor development. However, little is known about the role of this enzyme in proliferative tissues under physiological conditions. In the current work, we analysed the role of TIGAR in primary human lymphocytes stimulated with the mitotic agent Concanavalin A (ConA). We found that TIGAR expression was induced in stimulated lymphocytes through the PI3K/AKT pathway, since Akti-1/2 and LY294002 inhibitors prevented the upregulation of TIGAR in response to ConA. In addition, suppression of TIGAR expression by siRNA decreased the levels of the proliferative marker PCNA and increased cellular ROS levels. In this model, TIGAR was found to support the activity of glucose 6-phosphate dehydrogenase (G6PDH), the first enzyme of the pentose phosphate pathway (PPP), since the inhibition of TIGAR reduced G6PDH activity and increased autophagy. In conclusion, we demonstrate here that TIGAR is upregulated in stimulated human lymphocytes through the PI3K/AKT signaling pathway, which contributes to the redirection of the carbon flux to the PPP.

scholarly journals THE RÔLE OF ACETIC ACID IN THE BIOSYNTHESIS OF HEME

1950 ◽  
Vol 184 (2) ◽  
pp. 755-767 ◽  
Author(s):  
Norman S. Radin ◽  
D. Rittenberg ◽  
David Shemin
Keyword(s):  
Acetic Acid
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