The subcellular distribution of fatty acid components in ovine heart homogenate

1968 ◽  
Vol 46 (2) ◽  
pp. 121-128 ◽  
Author(s):  
A. R. Neill ◽  
C. J. Masters
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Subcellular Fractions ◽  
Gas Liquid Chromatography ◽  
Major Lipid Class ◽  
Differential Distribution ◽  
Subcellular Organelles ◽  
Heart Homogenate ◽  
Fatty Acid Compositions

The lipids of the subcellular fractions of ovine heart homogenate have been separated by chromatography on silicic acid into cholesteryl ester, glyceride and free fatty acid, and phospholipid fractions. Gas–liquid chromatography was used to determine the fatty acid compositions of these fractions.In all the subcellular organelles, phospholipid was the major lipid class, but in the cell sap fraction, glycerides predominated.By means of this lipid fractionation, and the presence in this tissue of two unusual major components, it has been possible to demonstrate a differential distribution of fatty acids between the separate subcellular fractions. The significance of this finding has been discussed in relation to the functions of lipids, the processes of fatty acid desaturation in the cell, and membrane biochemistry.The phospholipids of ovine mitochondria have been shown to be markedly saturated by comparison with the properties of this fraction in other mammals, and the implications of this lack of polyunsaturated components are considered in the Discussion.

THE COMPONENT FATTY ACIDS OF OILS FOUND IN SPORES OF PLANT RUSTS AND OTHER FUNGI. PART III

1964 ◽  
Vol 10 (3) ◽  
pp. 351-358 ◽  
Author(s):  
A. P. Tulloch ◽  
G. A. Ledingham
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Host Plants ◽  
Gas Liquid Chromatography ◽  
Axenic Cultures ◽  
Fatty Acid Compositions

The fatty acid compositions of the oils of several spore forms of seven species of Gymnosporangium were determined by gas–liquid chromatography. No simple relationships were found between the host plants and the compositions of the spore oils. cis-9, 10-Epoxyoctadecanoic acid was found in the teliospore oil of five of the species. The oil of basidiospores closely resembled that of teliospores but there was considerable variation in the oil of aeciospores. In one species the aeciospore and teliospore oils were very similar to each other, in another species there was partial resemblance, and in a third species they were different. The fats from four "axenic" cultures of Gymnosporangium juniperi-virginianae Schw. were analyzed, and were quite different from those of any of the rust species but resembled those frequently obtained from the vegetative stages of many species of fungi.

STEROL AND FATTY ACID COMPONENTS OF MYCELIUM OF FUSARIUM OXYSPORUM

1967 ◽  
Vol 13 (10) ◽  
pp. 1351-1355 ◽  
Author(s):  
A. N. Starratt ◽  
C. Madhosingh
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Fusarium Oxysporum ◽  
Culture Medium ◽  
Chloroform Extract ◽  
Gas Liquid Chromatography ◽  
Ergosterol Peroxide ◽  
Oleic And Linoleic Acids

A chloroform extract of the mycelium of Fusarium oxysporum Schl. em. Sny. et Hans, was analyzed for sterols; three major components were identified as ergosterol, ergosterol peroxide, and cerevisterol. The ratio of ergosterol to ergosterol peroxide formed was variable. Palmitic, stearic, oleic, and linoleic acids were identified by gas–liquid chromatography as the principal fatty acids of this organism.Fusaric acid, a phytotoxin previously obtained by other investigators from a number of forms of F. oxysporum, was isolated from the culture medium.

scholarly journals Studies on Fatty Acid Composition and Proximate Analyses of Anethum Sowa L. (Dill) Seed

1970 ◽  
Vol 42 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Md Moshfekus Saleh-E-In ◽  
Sudhangshu Kumar Roy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Gas Liquid Chromatography ◽  
Good Source ◽  
Anethum Sowa ◽  
Proximate Analyses

Anethum sowa L. (Dill) seeds were investigated to determine the fatty acid composition and proximate analyses. The seeds contain 9.36 % fatty oil. The saturated and unsaturated fatty acids contributed 6.22% and 93.78% respectively of the oil. The per cent composition of the extracted oil was identified by Gas Liquid Chromatography (GLC). Among the six fatty acids identified from this study oleic acid contributed the highest proportion (87.10%), where as, linolenic, palmitic, stearic, behenic and arachidic all together contributed the rest (12.90%). Proximate analyses showed that A. sowa. seeds are good source of dietary fibre. Overall Dill seeds oil can be considered as a good source of oleic acid. Key words: Anethum sowa, dill seed oil, fatty acid composition, oleic acid, linolenic acid, Gas liquid chromatography. Bangladesh J. Sci. Ind. Res. 42(4), 455-464, 2007

Influence of cytoplasms on the fatty acid composition of two maize inbred lines

1976 ◽  
Vol 86 (1) ◽  
pp. 151-154 ◽  
Author(s):  
P. Gregory ◽  
C. O. Grogan
Keyword(s):  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Lines ◽  
Gas Liquid Chromatography ◽  
Maize Seeds ◽  
Maize Inbred Lines ◽  
Oleic And Linoleic Acids ◽  
Fatty Acid Compositions

SUMMARYGas liquid chromatography was used to investigate the influence of several T, S and unclassified cytoplasms on the fatty acid composition of oil from A632 and CrS4HLA maize seeds. The fatty acid compositions of the A632 and CrS4HLA seeds differed markedly from each other with respect to oleic and linoleic acids but were stable within each seed line with respect to all sixteen of the cytoplasms tested.

FATTY ACID COMPOSITION OF NORMAL MINK TISSUES

1966 ◽  
Vol 44 (2) ◽  
pp. 179-185 ◽  
Author(s):  
B. L. Walker ◽  
V. F. Lishchenko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Fatty Acids ◽  
Methyl Esters ◽  
Dietary Factors ◽  
Gas Liquid Chromatography ◽  
Healthy Female ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

Lipids, extracted from the adrenals, brain, erythrocytes, heart, kidney, liver, plasma, and spleen of normal healthy female mink, were transesterified with 1% sulphuric acid in methanol, and the resulting methyl esters were analyzed by gas–liquid chromatography after purification by thin-layer chromatography. All of the tissues examined contained higher concentrations of unsaturated than of saturated acids, the highest levels of unsaturated acids occurring in the lipids of heart, adrenals, and plasma, and of the essential fatty acids (ω6 series, with six carbon atoms after last double bond) in plasma, erythrocyte, and kidney lipids. The fatty acid compositions of mink tissues resemble those reported in the literature for the rat; detailed comparisons are not possible because of the known influence of dietary factors on tissue fatty acids.

Comparison of fatty acid composition of stable L-phase variants of Staphylococcus aureus induced by three different mechanisms

1977 ◽  
Vol 23 (10) ◽  
pp. 1478-1481 ◽  
Author(s):  
Joseph MacGee ◽  
Cheryl Glotzbecker ◽  
Chatrchai Watanakunakorn
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Gas Liquid Chromatography ◽  
Fatty Acid Patterns ◽  
Phase Variants

The total saponifiable fatty acids of three stable L-phase variants of Staphylococcus aureus induced by cycloserine, methicillin, and lysostaphin were examined by gas-liquid chromatography. Five separate preparations of each of the three variants were examined. Twenty-nine fatty acids were identified. The fatty acid patterns of the three variants were very similar.

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