CELL-FREE INCORPORATION OF AMINO ACIDS BY A TROUT-LIVER SYSTEM. HEAT-LABILE AMINOACYL TRANSFERASE ACTIVITY

1967 ◽  
Vol 45 (12) ◽  
pp. 2005-2014 ◽  
Author(s):  
Lawrence Rosen ◽  
E. L. Murray ◽  
G. David Novelli
Keyword(s):  
Amino Acids ◽  
Rat Liver ◽  
Transferase Activity ◽  
Free System ◽  
Cell Free System ◽  
Heat Labile ◽  
A Cell ◽  
Aminoacyl Transferase

A cell-free system from trout liver that incorporates amino acids into protein has been isolated. The system requires both soluble and particulate fractions, and is similar to those isolated from other organisms.The incorporation of amino acids is extremely heat-labile, and this lability has been localized in the aminoacyl transferase activity of the cell sap. The heat lability of the microsomes may also be related to transferase activity.Ribosomal fractions from trout liver and rat liver incorporate amino acids when incubated with cell sap from rat liver and trout liver, respectively.

scholarly journals Trafficking of lipids from the endoplasmic reticulum to the Golgi apparatus in a cell-free system from rat liver

1991 ◽  
Vol 266 (7) ◽  
pp. 4322-4328 ◽  
Author(s):  
P Moreau ◽  
M Rodriguez ◽  
C Cassagne ◽  
D M Morré ◽  
D J Morré
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Rat Liver ◽  
Free System ◽  
Cell Free System ◽  
A Cell

Studies on the Incorporation of Amino Acids by a Cell-Free System Obtained from Beef Retina

1972 ◽  
Vol 50 (5) ◽  
pp. 581-587 ◽  
Author(s):  
Y. Matuk
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Incubation Medium ◽  
Optimum Concentration ◽  
Free System ◽  
Cell Free System ◽  
A Cell

The incorporation of 14C-leucine into proteins by a cell-free system from beef retina was studied. It was found that the optimum concentration of ATP depended on the concentration of ribosomes in the incubation medium. Very little incorporation of 14C-leucine was observed in the absence of K+. The optimum concentration of phosphocreatine required for incorporation of radioactive leucine depended on the concentration of Mg2+ in the incubation medium, and the optimum concentration of K+ appears to be independent of the concentrations of Mg2+ and phosphocreatine used.Retinol and retinal had no effect, but ethanol markedly inhibited protein synthesis at concentrations higher than 2%.Puromycin (10−4 M) inhibited incorporation of 14C-leucine by about 80%. The degree of inhibition by cycloheximide depended on the concentration of pH 5 fraction in the incubation medium.

Vitamin K-dependent formation of factor VII by a cell-free system from rat liver

Biochemistry ◽  
1970 ◽  
Vol 9 (13) ◽  
pp. 2564-2569 ◽  
Author(s):  
Bernard M. Babior ◽  
Ruby S. Kipnes
Keyword(s):  
Rat Liver ◽  
Vitamin K ◽  
Factor Vii ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Poly(U, s4U) as a synthetic messenger RNA for polyphenylalanine synthesis in a cell-free system derived from rat liver

1975 ◽  
Vol 2 (4) ◽  
pp. 595-601
Author(s):  
M. Keren-Zur ◽  
A.A. Hochberg ◽  
N. de Groot ◽  
Y. Lapidot
Keyword(s):  
Rat Liver ◽  
Messenger Rna ◽  
Free System ◽  
Cell Free System ◽  
A Cell
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