THE INDUCTION OF NAD KINASE BY DDT IN TRIATOMA INFESTANS

1967 ◽  
Vol 45 (5) ◽  
pp. 619-626 ◽  
Author(s):  
M. Agosin ◽  
Jovita Ilivicky ◽  
S. Litvak
Keyword(s):  
Column Chromatography ◽  
De Novo ◽  
Deae Cellulose ◽  
Triatoma Infestans ◽  
Protective Mechanism ◽  
Nad Kinase ◽  
De Novo Synthesis ◽  
Enzyme Protein ◽  
Sephadex Column ◽  
Immunochemical Techniques

The NAD kinase (EC 2.7.1.23) from Triatoma infestans has been purified and a specific antiserum against it prepared. Immunochemical techniques have shown that the increase in the levels of NAD kinase in nymphs of T. infestans is accompanied by an increase in the amount of enzyme protein. The enzyme is labeled after injection of 14C-labeled leucine in both induced and non-induced insects, but labeling is greater in the former, which further supports the concept that a de novo synthesis of enzyme protein occurs during induction by DDT. The enzyme is heterogeneous by DEAE-cellulose and DEAE-Sephadex column chromatography, but the antiserum does not distinguish this heterogeneity. NAD kinase induction may correspond to a protective mechanism of the insects by increasing the availability of coenzymes required for DDT detoxication.

Effect of wounding on the synthesis of phenols, phenol oxidase, and peroxidase in the tuber tissue of Jerusalem artichoke

1968 ◽  
Vol 46 (11) ◽  
pp. 1339-1343 ◽  
Author(s):  
Marcel Bastin
Keyword(s):  
Phenolic Acids ◽  
De Novo ◽  
Deae Cellulose ◽  
Jerusalem Artichoke ◽  
Phenol Oxidase ◽  
De Novo Synthesis ◽  
Complete Darkness ◽  
Tuber Tissue

Slices of tubers of Jerusalem artichoke were cultured at 28 °C in complete darkness, and the production of phenolic acids, phenol oxidase, and peroxidase was studied in relation to subsequent cutting of the tissue. Wounding increased the production of both phenolic acids and enzymes. Purification of the peroxidase by DEAE-cellulose chromatography showed that not all of its components were synthesized in the injured slices. Cycloheximide and chloramphenicol were added to cultures to investigate a requirement for the de novo synthesis of protein in relation to production of the compounds. Cycloheximide at a concentration of 5 μg/ml strongly inhibited the production of both phenolic acids and the enzymes, but chloramphenicol decreased the production of only phenol oxidase.

On the purification of a fatty acid synthetase complex from an insect

1976 ◽  
Vol 54 (8) ◽  
pp. 1397-1399 ◽  
Author(s):  
S. N. Thompson ◽  
J. S. Barlow
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Column Chromatography ◽  
De Novo ◽  
Gel Filtration ◽  
Fatty Acid Synthetase ◽  
De Novo Synthesis ◽  
Anion Exchange Column ◽  
Malonyl Coa ◽  
Salt Fractionation

The fatty acid synthetase complex of the blowfly, Lucilia sericata, catalyzing the de novo synthesis of fatty acids from acetyl- and malonyl-CoA (EC 2.3.1.9 and EC 2.3.1.39 respectively) and requiring NADPH, was isolated from whole-insect homogenates by ultracentrifugation. Purification was carried out by salt fractionation, anion exchange column chromatography on ECTEOLA (epichlorohydrin triethanolamine) cellulose, and gel filtration column chromatography on Sephadex®.

Evidence for Increased de Novo Synthesis of NAD in Immune-Activated RAW264.7 Macrophages: A Self-Protective Mechanism?

1999 ◽  
Vol 372 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Ross S. Grant ◽  
Robert Passey ◽  
Gabrijela Matanovic ◽  
George Smythe ◽  
Vimal Kapoor
Keyword(s):  
De Novo ◽  
Protective Mechanism ◽  
De Novo Synthesis ◽  
Raw264.7 Macrophages

Characterization of Monocyte-Associated Factor V

1993 ◽  
Vol 70 (02) ◽  
pp. 273-280 ◽  
Author(s):  
Janos Kappelmayer ◽  
Satya P Kunapuli ◽  
Edward G Wyshock ◽  
Robert W Colman
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Peripheral Blood ◽  
De Novo ◽  
Factor V ◽  
Blood Monocytes ◽  
De Novo Synthesis ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Peripheral Blood Monocytes

SummaryWe demonstrate that in addition to possessing binding sites for intact factor V (FV), unstimulated peripheral blood monocytes also express activated factor V (FVa) on their surfaces. FVa was identified on the monocyte surface by monoclonal antibody B38 recognizing FVa light chain and by human oligoclonal antibodies H1 (to FVa light chain) and H2 (to FVa heavy chain) using immunofluorescence microscopy and flow cytometry. On Western blots, partially cleaved FV could be identified as a 220 kDa band in lysates of monocytes. In addition to surface expression of FVa, monocytes also contain intracellular FV as detected only after permeabilization by Triton X-100 by monoclonal antibody B10 directed specifically to the Cl domain not present in FVa. We sought to determine whether the presence of FV in peripheral blood monocytes is a result of de novo synthesis.Using in situ hybridization, no FV mRNA could be detected in monocytes, while in parallel control studies, factor V mRNA was detectable in Hep G2 cells and CD18 mRNA in monocytes. In addition, using reverse transcriptase and the polymerase chain reaction, no FV mRNA was detected in mononuclear cells or in U937 cells, but mRNA for factor V was present in Hep G2 cells using the same techniques. These data suggest that FV is present in human monocytes, presumably acquired by binding of plasma FV, and that the presence of this critical coagulation factor is not due to de novo synthesis.

Platelets Stimulate Thromboplastin Synthesis in Human Endothelial Cells

1983 ◽  
Vol 49 (02) ◽  
pp. 069-072 ◽  
Author(s):  
U L H Johnsen ◽  
T Lyberg ◽  
K S Galdal ◽  
H Prydz
Keyword(s):  
Endothelial Cells ◽  
De Novo ◽  
Umbilical Vein ◽  
Time Dependent ◽  
De Novo Synthesis ◽  
Human Endothelial Cells ◽  
Tumor Promotor ◽  
Coagulation Assay ◽  
Platelet Aggregates ◽  
Umbilical Vein Endothelial Cells

SummaryHuman umbilical vein endothelial cells in culture synthesize thromboplastin upon stimulation with phytohaemagglutinin (PHA) or the tumor promotor 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The thromboplastin activity is further strongly enhanced in a time dependent reaction by the presence of gel-filtered platelets or platelet aggregates. This effect was demonstrable at platelet concentrations lower than those normally found in plasma, it may thus be of pathophysiological relevance. The thromboplastin activity increased with increasing number of platelets added. Cycloheximide inhibited the increase, suggesting that de novo synthesis of the protein component of thromboplastin, apoprotein III, is necessary.When care was taken to remove monocytes no thromboplastin activity and no apoprotein HI antigen could be demonstrated in suspensions of gel-filtered platelets, platelets aggregated with thrombin or homogenized platelets when studied with a coagulation assay and an antibody neutralization technique.

DE NOVO SYNTHESIS OF STEROIDS BY THE TESTES OF THE HUMAN FOETUS AT MIDGESTATION

1971 ◽  
Vol 68 (1_Supplb) ◽  
pp. S135 ◽  
Author(s):  
R. S. Mathur ◽  
N. Wiqvist ◽  
E. Diczfalusy
Keyword(s):  
De Novo ◽  
De Novo Synthesis ◽  
Human Foetus

DESCRIÇÃO DE FOCOS RESIDUAIS DE TRIATOMA INFESTANS (KLUG, 1834) NO MUNICÍPIO DE NOVO HORIZONTE, BAHIA

1970 ◽  
Vol 39 ◽  
pp. 91
Author(s):  
Helena Brandão ◽  
Eduardo Fonseca ◽  
Roberto Santos ◽  
Gilmar Ribeiro Júnior ◽  
Carlos Gustavo Santos ◽  
...  
Keyword(s):  
De Novo ◽  
Triatoma Infestans

Triatoma infestans foi considerado, por anos, o principal vetor da doença deChagas no Brasil. A redução dos índices de infestação predial associados à espécie conferiuao país, em 2006, certificação de eliminação da transmissão da doença por esse vetor.Porém, focos residuais foram encontrados no município de Novo Horizonte (BA), Brasil, apartir do ano de 2010. Diante desse panorama, foi desenvolvido um estudo com objetivode descrever os focos residuais de T. infestans no município de Novo Horizonte, área decaatinga da Bahia. As pesquisas foram realizadas na localidade de Fazenda Queimadas6ª, zona rural do município, nos anos de 2011 a 2013. Os triatomíneos coletados foramidentificados, triados e dissecados para diagnóstico molecular da infecção natural peloTrypanosoma cruzi e avaliação de suas fontes alimentares. Durante o estudo, foramcoletados 502 exemplares, distribuídos em dois focos residuais: em 2011 com 71 indivíduosno intra e peridomicílio; e, em 2013, com 431, apenas no peridomicílio. Desse total, 143exemplares foram selecionados para análises moleculares: todos os coletados em 2011 e 16,7% dos coletados em 2013. O Índice de Infecção Natural pelo T. cruzi foi de 4,2%. O DNA de ave foi detectado em 60% dos triatomíneos analisados, 1 infectado por T. cruzi. Já o DNA humano ocorreu em 3% das amostras, com nenhum triatomíneo infectado. A convivência de seres humanos e animais domésticos com colônias de T. infestans suscita a possibilidade de recolonização dessa espécie na Bahia, aumentando a chance de transmissão vetorial da doença de Chagas.

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