A TECHNIQUE OF PREPARATIVE PAPER CHROMATOGRAPHY

1966 ◽  
Vol 44 (2) ◽  
pp. 149-153 ◽  
Author(s):  
A. E. Pasieka ◽  
J. E. Logan
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Biological Materials ◽  
High Salt ◽  
Salt Medium ◽  
Preparative Scale ◽  
High Concentrations ◽  
Total Amino Acids

The use of a solvent redevelopment technique enables the separation of amino acids from complex biological materials in the presence of high concentrations of salts. By conventional chromatography, 1.17 mg of total amino acids have been separated from the high salt medium M 150. The preparative technique as described here has separated amounts as great as 1.17 g and the patterns are essentially the same as for the analytical types. The separations are effected by four or more successive 15- to 20-hour solvent developments with drying between each solvent stage before the staining of chromatograms or isolation of particular bands. The results of these solvent developments on the preparative scale are illustrated with photographs of actual chromatograms. This technique requires thick filter paper sheets up to 4 ft in length for analytical, and particularly for preparative, chromatograms.

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (1) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

Buffered Filter Paper Chromatography of Amino Acids

1951 ◽  
Vol 23 (1) ◽  
pp. 168-174 ◽  
Author(s):  
E. F. McFarren
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper

Destruction of Amino Acids during Filter Paper Chromatography

Science ◽  
1951 ◽  
Vol 113 (2923) ◽  
pp. 4-6 ◽  
Author(s):  
M. K. Brush ◽  
R. K. Boutwell ◽  
A. D. Barton ◽  
C. Heidelberger
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper

A VERSATILE HIGH-VOLTAGE PAPER ELECTROPHORESIS APPARATUS AND ITS APPLICATION TO COMPLEX BIOLOGICAL MATERIALS

1961 ◽  
Vol 39 (8) ◽  
pp. 1313-1324 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
High Voltage ◽  
Filter Paper ◽  
Heat Exchangers ◽  
Cooling System ◽  
Paper Electrophoresis ◽  
Biological Materials ◽  
Heat Conductance ◽  
Cooling Plate ◽  
External Cooling

Improvements on a high-voltage paper electrophoresis apparatus are described. The major improvements consist of a simplified cooling system and modification of the cooling plate to accommodate filter paper sheets 24 × 48 in. in size. Two methods, both employing solid heat exchangers, have been devised for the dissipation of Joule's heat: (a) external cooling, employing a Freon 12 circulating coolant and (b) no external cooling, but with heat conductance and radiation by means of an aluminum plate and metal rail.The application of this modified apparatus to the separation of amino acids and peptides from complex biological materials is illustrated.

Research Regarding Content in Amino-acids and Biological Value of Proteins from Polyodon spathula Sturgeon Meat

2017 ◽  
Vol 68 (5) ◽  
pp. 1063-1069
Author(s):  
Cristina Simeanu ◽  
Daniel Simeanu ◽  
Anca Popa ◽  
Alexandru Usturoi ◽  
Dan Bodescu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nutritive Value ◽  
Essential Amino Acids ◽  
Meat Production ◽  
Age Group ◽  
Chemical Methods ◽  
Biological Value ◽  
Polyodon Spathula ◽  
The One ◽  
Total Amino Acids

Polyodon spathula sturgeon breed is successfully reared in Romania in many fishery farms for meat production and it is capitalized on domestic market as consumption fish. In the current paper were studied a number of 1400 sturgeons from Polyodon spathula breed (1200 individuals of one summer - P.s.0+ and 200 individuals of fourth summers - P.s.3+). From this flock were weighted around 10%, for each age group, and for laboratory determinations were chosen 10 fishes for each age with the corporal mass close to the group mean. After analysing the fillets gathered from the studied fishes for establishing the chemical characteristics, nutritive and biological values of proteins were drawn some interesting conclusions. So, regarding chemical composition we notice that in the meat of analysed fishes water is in a rate of 75.41% at P.s.3+ and 78.37% for P.s.0+; proteins - between 18.08% for P.s.0+ and 19.89% for P.s.3+, values which place those fishes in the group of protein fishes; lipids - between 2.45% and 3.45%, values which situated those sturgeons in category of fishes with a low content in lipids; collagen � 3.83% at P.s.0+ and 4.14% at P.s.3+ which indicate low values for proteins of weak quality in the meat of those sturgeons. Study of nutritive value for the analysed fishes indicate the fact that fishes P.s.0+ have a mediocre nutritive value, having the ratio w/p of 4.33 while sturgeons P.s.3+ were placed in the 2nd category � fishes with a good nutritive value (rate w/p = 3.79). Energetic value of the studied fillets was 97.39 kcal/100 g for P.s.0+ and 114.31 kcal/100 g for P.s.3+, which enlightened an increase of nutritive value with aging, fact especially due to accumulation of adipose tissue. Study of proteins quality, through the presence of those 8 essential amino-acids in the meat of analysed fishes, show the fact that at sturgeons P.s.0+ proportion of essential amino-acids was 20.88% from total amino-acids, while at sturgeons P.s.3+ was 26.23%, fact which enlightened an increasing of proteins� biological value with fish aging. This fact was also shown by calculation of proteins� biological value through chemical methods (EAA index); calculated value for sturgeons P.s.0+ was a little bit lower (118.73) than the one calculated for sturgeons P.s.3+ (118.79).

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