NUCLEIC ACID OF MURINE ENCEPHALOMYELITIS VIRUS PHYSICAL AND CHEMICAL STUDIES

1965 ◽  
Vol 43 (11) ◽  
pp. 1829-1838
Author(s):  
M. A. Moscarello
Keyword(s):  
Nucleic Acid ◽  
Sucrose Gradient ◽  
Extraction Procedure ◽  
Encephalomyocarditis Virus ◽  
Minor Components ◽  
Snake Venom Phosphodiesterase ◽  
Chemical Studies ◽  
Encephalomyelitis Virus ◽  
Physical And Chemical ◽  
Degree Of Fragmentation

Studies concerning some of the chemical and physical properties of ribonucleates isolated from highly purified encephalomyocarditis virus are presented. Base analyses were performed by both an alkali degradation procedure and hydrolysis with snake venom phosphodiesterase. In addition to the four common bases, adenine, guanine, cytosine, and uracil, several minor components have been found in both the alkali and diesterase hydrolysates. Since much of the work was done with P32-labelled ribonucleates, it has not been possible to identify any of the minor components.Ultracentrifugal studies performed in the Spinco model E analytical centrifuge revealed a single peak corresponding to a S20, w of 3.2. This low S20, w was confirmed by centrifugation in a sucrose gradient. These results, in addition to those obtained by column chromatography, have been interpreted as being indicative of a considerable degree of fragmentation, probably as a result of the presence of trace amounts of ribonucleases or hydroxyl ions during the extraction procedure.

scholarly journals Composition of Pseudomonas aeruginosa slime

1969 ◽  
Vol 112 (4) ◽  
pp. 521-525 ◽  
Author(s):  
M. R. W. Brown ◽  
J. H. Scott Foster ◽  
J. R. Clamp
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Hyaluronic Acid ◽  
Nucleic Acid ◽  
Growth Medium ◽  
Extraction Procedure ◽  
The Other ◽  
Minor Components ◽  
Polysaccharide Fraction ◽  
Defined Media ◽  
Rna And Dna

1. The slime produced by eight strains of Pseudomonas aeruginosa on a number of different media was demonstrated to be qualitatively the same. Small quantitative differences may be occasioned by differences in the extraction procedure, the growth medium or the strain of organism used. 2. The slime was shown to be predominantly polysaccharide with some nucleic acid material and a small amount of protein. 3. The hydrolysed polysaccharide fraction consists mainly of glucose with smaller amounts of mannose. This accounts for some 50–60% of the total slime. In addition, there is some 5% of hyaluronic acid. The nucleic acid material represents approx. 20% of the total weight, and is composed of both RNA and DNA. 4. Minor components are protein, rhamnose and glucosamine, the protein being less than 5% of the total. 5. Hyaluronic acid is produced in greater quantities from nutrient broth than from chemically defined media, and is more firmly attached to the cells than the other components.

scholarly journals Physical and Chemical Studies on Ceruloplasmin

1965 ◽  
Vol 240 (5) ◽  
pp. 1974-1978 ◽  
Author(s):  
Philip Aisen ◽  
Anatol G. Morell
Keyword(s):  
Chemical Studies ◽  
Physical And Chemical

scholarly journals Physical and Chemical Studies of a Limited Reaction of Iodine with Proteins

1959 ◽  
Vol 234 (6) ◽  
pp. 1447-1451
Author(s):  
Leon W. Cunningham ◽  
B. Joanne Nuenke
Keyword(s):  
Chemical Studies ◽  
Physical And Chemical

scholarly journals Physical and Chemical Studies on Ceruloplasmin

1969 ◽  
Vol 244 (13) ◽  
pp. 3494-3496 ◽  
Author(s):  
A G Morell ◽  
C J A Van Den Hamer ◽  
I H Scheinberg
Keyword(s):  
Chemical Studies ◽  
Physical And Chemical

Ionic Liquids in Analytical Chemistry: Applications and Recent Trends

2021 ◽  
Vol 17 ◽  
Author(s):  
Giuseppe Maria Merone ◽  
Angela Tartaglia ◽  
Enrica Rosato ◽  
Cristian D’Ovidio ◽  
Abuzar Kabir ◽  
...  
Keyword(s):  
Analytical Chemistry ◽  
Ionic Liquids ◽  
Stationary Phases ◽  
Extraction Procedure ◽  
Chemical Properties ◽  
Electrochemical Analysis ◽  
Physico Chemical ◽  
Recent Trends ◽  
Number Of Publications ◽  
Physical And Chemical

Background: Ionic liquids (ILs) are a unique class of compounds consisting exclusively of cations and anions that possess distinctive properties such as low volatility, high thermal stability, miscibility with water and organic solvents, electrolytic conductivity and non-flammability. Ionic liquids have been defined as "design solvents", because it is possible to modify their physical and chemical properties by appropriately choosing cations and anions, in order to meet the specific characteristics based on their potential application. Introduction: Due of their tunable nature and properties, ILs are considered as the perfect candidates for numerous applications in analytical chemistry including sample preparation, stationary phases in liquid or gas chromatography, additives in capillary electrophoresis, or in mass spectrometry for spectral and electrochemical analysis. In the last years, the number of publications regarding ILs has rapidly increased, highlighting the broad applications of these compounds in various fields of analytical chemistry. Results: This review first described the main physico-chemical characteristics of ionic liquids, and subsequently reported the various applications in different subdisciplines of analytical chemistry, including the extraction procedure and separation techniques. Furthermore, in each paragraph the most recent applications of ionic liquids in the food, environmental, biological, etc. fields have been described. Conclusion: Overall, the topic discussed highlights the key role of ionic liquids in analytical chemistry, giving hints for their future applications in chemistry but also in biology and medicine.

scholarly journals PHYSICAL AND CHEMICAL STUDIES OF OVERROOF ROCKS OF CLAY SHALES OF THE DZHERDANAK DEPOSIT

2021 ◽  
pp. 16-21
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Chemical Properties ◽  
Mineralogical Composition ◽  
Physical And Chemical Properties ◽  
X Ray ◽  
Clay Shales ◽  
Chemical Studies ◽  
Physical And Chemical ◽  
And Chemical Properties

The purpose of this study is study of the physical and chemical properties of the overburden of the Dzherdanak deposit. The chemical and mineralogical composition of the overburden of the Djerdanak deposit has been studied by the methods of X-ray and thermography, electron microscopy and infrared spectroscopy. The main phases are quartz, kaolinite and muscovite. The study of the fine structure of the rock under an electron microscope showed the homogeneity of the rock with pronounced uniform inclusions, which is preserved even after firing. Changes in the rock after firing at 1050 °C have been determined. The formation of mullite at this temperature has been established.

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