STUDIES ON THE LIVETINS OF HEN'S EGG YOLK: II. IMMUNOELECTROPHORETIC IDENTIFICATION OF LIVETINS WITH SERUM PROTEINS

1964 ◽  
Vol 42 (8) ◽  
pp. 1119-1131 ◽  
Author(s):  
Chi-Ching Mok ◽  
R. H. Common
Keyword(s):  
Serum Albumin ◽  
Filter Paper ◽  
Laying Hens ◽  
Serum Proteins ◽  
Egg Yolk ◽  
Laying Hen ◽  
Immunoelectrophoretic Analysis ◽  
Hen’S Egg ◽  
Additional Antigen ◽  
Serum Antigens

Ten antigens have been distinguished in the sera of cocks and non-laying hens by immunoelectrophoretic analysis (IEA) against homologous rabbit antisera. An additional antigen has been found in the sera of laying hens by IEA against anti-laying-hen serum.Four of the serum antigens obtained by IEA have been correlated electrophoretically with the filter paper electrophoretic fractions serum albumin, serum α2-globulin, serum β-globulin, and serum γ-globulin.Serum albumin, serum α1-globulin, serum β-globulin, and serum γ-globulin have been identified immunologically with alpha-livetin, a livetin antigen (livetin 2), gamma1-livetin and gamma2-livetin respectively. Another antigen of cock serum and hen serum has been identified by IEA with a livetin antigen detected by IEA of livetin against anti-cock, anti-hen, and anti-yolk sera.

scholarly journals Calcium deposition in egg due to substitution of limestone by eggshell flour in feed of laying hens

2018 ◽  
Vol 43 (3) ◽  
pp. 257 ◽  
Author(s):  
S. Kismiati ◽  
T. Yuwanta ◽  
Z. Zuprizal ◽  
S. Supadmo ◽  
U. Atmomarsono
Keyword(s):  
Laying Hens ◽  
Egg Yolk ◽  
Laying Hen ◽  
Calcium Deposition ◽  
Experimental Unit ◽  
Egg Weight ◽  
Tibia Bone ◽  
Randomized Design

The aim of this research was to evaluate calcium deposition in egg using eggshell flour as a limestone substitute in feed. Two hundreds laying hen of Isa Brown strain of 25 weeks were used in this study. Treatments were diet with 7.5% limestone as control (T0), 2.5% limestone is substituted with eggshell flour(T1), 5% limestone is substituted with eggshell flour (T2) and limestone is substituted with eggshell flour (T3). A completly randomized design were used to allocated the treatments with 5 replications of each. Each experimental unit consists of 10 laying hens. Parameters measured were egg weight, yolk weight, albumen weight, eggshell weight, calcium of egg (yolk, albumen and eggshell), length, weight and Ca of tibia bone. The results showed that substitution of limestone with eggshell flour had significantly effect (P<0.05) on eggshell weight, Ca deposition on yolk, albumen, and Ca of tibia bone but non significantly effect on egg weight, weight and percentage of yolk, weight and percentage of albumen, percentage and Ca of eggshell, length and weight of tibia bone. In conclusion, calcium deposition in yolk was the highest in the use of 7.5% eggshell flour to substitute limestone but obtained the lowest Ca of bone , while calcium deposition in albumen was the highest in the use of 2.5% eggshell flour.

STUDIES ON THE LIVETINS OF HEN'S EGG YOLK: I. IDENTIFICATION OF PAPER-ELECTROPHORETIC AND IMMUNOELECTRO-PHORETIC LIVETIN FRACTIONS WITH SERUM PROTEIN ANTIGENS BY IMMUNOELECTROPHORETIC ANALYSIS

1964 ◽  
Vol 42 (6) ◽  
pp. 871-881 ◽  
Author(s):  
Chi-Ching Mok ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Egg Yolk ◽  
Paper Electrophoresis ◽  
Protein Antigens ◽  
Immunoelectrophoretic Analysis ◽  
Hen’S Egg ◽  
Alpha Beta

Immunoelectrophoretic analysis of an egg yolk livetin preparation has demonstrated the presence of six distinct fractions, which were numbered 1, 2, 3, 4, 5, and 6 from the anodic end of the electropherogram. Four of these fractions have been identified with four livetin fractions obtained by paper electrophoresis as follows: immunoelectrophoretic fraction 1 with alpha-livetin, fraction 2 with beta-livetin, fraction 4 with gamma1-livetin, and fraction 5 with gamma2-livetin.Immunoelectrophoresis of hen serum or cock serum against antilivetin serum has shown that both hen and cock sera contain antigens electrophoretically and immunologically identical to alpha-, beta-, gamma1-, and gamma2-livetins.

STUDIES ON THE LIVETINS OF EGG YOLK: III. HETEROGENEITY AND IMMUNOELECTROPHORETIC BEHAVIOR OF BETA-LIVETIN

1966 ◽  
Vol 44 (10) ◽  
pp. 1357-1364 ◽  
Author(s):  
Shun-Fong Hui ◽  
R. H. Common
Keyword(s):  
Serum Albumin ◽  
Gel Electrophoresis ◽  
Egg Yolk ◽  
The Other ◽  
Positive Staining ◽  
Staining Reaction ◽  
Starch Gel Electrophoresis ◽  
Hen’S Egg ◽  
Starch Gel ◽  
The One

Starch-gel electrophoresis of the total livetins of hen's egg yolk resolved 16 zones: seven major zones, six minor zones, and three faint, diffuse zones. One zone was identified with the major component of paper electrophoretic alpha-livetin and hence with serum albumin. Four of the major zones were identified with the major components of paper electrophoretic beta-livetin on the one hand, and with an electrophoretically heterogeneous livetin antigen (livetin antigen 3) on the other hand, thus establishing the electrophoretic heterogeneity and relative immunological homogeneity of the paper electrophoretic beta-livetin fraction. The other two major starch-gel electrophoretic zones were identified as transferrins by their positive staining reaction for iron and comparison of their mobilities with two corresponding serum starch-gel fractions.

The Development of a Quantitative ELISA for Aflatoxin B1 in Ground Peanuts Using Antibodies Isolated From the Yolk of a Laying Hen

1994 ◽  
Vol 57 (11) ◽  
pp. 1022-1024 ◽  
Author(s):  
SUZHEN LI ◽  
RONALD R. MARQUARDT ◽  
ANDREW A. FROHLICH ◽  
HAO XIAO ◽  
JAMES R. CLARKE
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Laying Hens ◽  
Egg Yolk ◽  
Laying Hen ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coating Antigen ◽  
Extraction Protocol

Antibodies directed against Aflatoxin B1 (AFB1) were produced in laying hens, isolated from egg yolk and applied in an enzyme-linked immunosorbent assay (ELISA) for AFB1 in ground peanuts. The ELISA sensitivity was improved by reduction of the amount of the coating antigen absorbed onto the microplate well surfaces. The main aflatoxins B1, B2, G1, G2, M1, aflatoxicol, sterigmatocystin were found to cross-react in the competitive ELISA, 100, 25, 23, 4, 0, 0, 0%, respectively. Aflatoxin B1 could be reproducibly detected and quantified in spiked ground peanuts at levels greater than 5 ppb using a hexane and methanol solvent based peanut extraction protocol.

scholarly journals ELECTROPHORESIS OF PROTEINS ON FILTER PAPER

1951 ◽  
Vol 35 (1) ◽  
pp. 89-118 ◽  
Author(s):  
Henry G. Kunkel ◽  
Arne Tiselius
Keyword(s):  
Serum Albumin ◽  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Normal Serum ◽  
Concentration Gradients ◽  
Simplified Procedure ◽  
Highly Porous ◽  
Electrophoresis Of Proteins ◽  
Serum Components

A simplified procedure for filter paper electrophoresis is described in which disturbing factors such as evaporation, heating, buffer concentration gradients, and pH changes in the electrode vessels were reduced to a minimum. Artificial mixtures of highly purified proteins could be separated and the components isolated. The application of the method to a variety of studies on serum proteins is demonstrated. Protein concentration in paper segments was determined by two different methods of protein estimation. Curves were obtained showing the same five major peaks for normal serum as found by the classical methods of free electrophoresis. Comparisons were made of the areas of the various components under the curves obtained with the different methods. Two dimensional electrophoresis was applied to serum and serum components. It proved of value in demonstrating the heterogeneity of fractions such as the γ-globulin of serum. The polysaccharide dextran was used as an index of the extent of electro-osmotic flow during the course of the various experiments. The ratio of the distance of electroosmotic flow and the distance of protein migration was shown to be constant for a given type of paper. For serum albumin on Munktell 20 paper this ratio was 0.35. A formula for mobilities applicable to liquid in a highly porous supporting medium is presented. Mobility values for human serum albumin at various pH levels on paper showed approximate agreement with those obtained in free solution giving a similar isoelectric point.

scholarly journals Serum proteins and the livetins of hen's-egg yolk

1962 ◽  
Vol 83 (2) ◽  
pp. 346-355 ◽  
Author(s):  
J WILLIAMS
Keyword(s):  
Serum Proteins ◽  
Egg Yolk ◽  
Hen’S Egg

scholarly journals Pequi peel meal in laying hen diet

2016 ◽  
Vol 38 (2) ◽  
pp. 151 ◽  
Author(s):  
Amanda Lapa Silva ◽  
Romênia Regina Pires Lage ◽  
Daniel Emygdio de Faria Filho ◽  
Izabela Lorena Azevedo ◽  
Adélio Nunes Dias ◽  
...  
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Egg Yolk ◽  
Laying Hen ◽  
The Other ◽  
Egg Mass ◽  
Feed Conversion ◽  
Randomized Design ◽  
Completely Randomized Design

This study evaluated the effect of pequi peel meal in the diet for laying hens on performance and egg quality. Hy-Line® Brown hens (n=160) aged 55 weeks with 1642±63g body weight were distributed in a completely randomized design with five treatments and four replications of eight birds each. The treatments consisted of isometric substitution of corn by pequi peel meal at 0, 2.5, 5.0, 7.5 and 10%. The performance and internal and external egg quality were evaluated. Egg production, egg mass and feed conversion worsened linearly with increasing replacement of corn with pequi peel meal, while the yolk color increased linearly. The other variables related to performance and internal and external egg quality were not significantly affected by the treatments. It can be concluded that the use of pequi peel meal replacing corn in laying hen diet does not change the internal and external egg quality, improves the pigmentation of egg yolk, and impairs performance of laying hens. 

scholarly journals Immunological Comparison of Native and Recombinant Hen’s Egg Yolk Allergen, Chicken Serum Albumin (Gal d 5), Produced in Kluveromyces lactis

Nutrients ◽  
2018 ◽  
Vol 10 (6) ◽  
pp. 757 ◽  
Author(s):  
Chamika De Silva ◽  
Pathum Dhanapala ◽  
Samuel King ◽  
Timothy Doran ◽  
Mimi Tang ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Egg Yolk ◽  
Chicken Serum ◽  
Hen’S Egg ◽  
Immunological Comparison

Study of Fatty Acids Profile and Oxidative Stability of Egg Yolk from Hens Fed a Diet Containing White Lupine Seeds Meal

2018 ◽  
Vol 69 (9) ◽  
pp. 2454-2460 ◽  
Author(s):  
Florin Leontin Criste ◽  
Daniel Mierlita ◽  
Daniel Simeanu ◽  
Paul Corneliu Boisteanu ◽  
Ioan Mircea Pop ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oxidative Stability ◽  
Soybean Meal ◽  
Laying Hens ◽  
Control Diet ◽  
Egg Yolk ◽  
Laying Hen ◽  
Partial Replacement ◽  
Fatty Acids Profile ◽  
White Lupine

The aim of this study was to evaluate the effects of different dietary inclusion of raw white lupine seed meal (WL) in diets of laying hen on fatty acids (FA) profile, health lipid indices and oxidative stability of egg yolk. A total of 160 TETRA-SL LL laying hens (30-week old) were used in a ten week trial. The laying hens were randomly assigned to four dietary treatments: control diet which contained soybean meal (C), and study diets in which soybean meal was replaced with WL at 150 g/kg (E15), 200 g/kg (E20) and 250 g/kg (E25). Each treatment was replicated 5 times with 8 birds each. The inclusion of WL in experimental diets caused a linear increase in n-6 and n-3 polyunsaturated FA (PUFA) content and a decrease in monounsaturated FA (MUFA), but it had no influence on the saturated FA (SFA) of egg yolk lipids. The data suggested that the partial replacement of soybean meal with WL of in laying hen diet it significant increase h/H (hypocholesterolemic/Hypercholesterolemic) and PUFA/SFA ratio, and decrease thrombogenic index (TI), which improves human health because of the beneficial effect on the cardiovascular system. The authors determined the concentration of a-tocopherol by high performance liquid chromatography (HPLC) and assessed the degree of oxidative degradation of lipids using the classical test based on the concentration of malondialdehyde (MDA). Inclusion of WL in diets led to increases in a-tocopherol and decrease in MDA in eggs (P [0.05). Egg storage for 20 d or 40 d led to linear reduction in egg �-tocopherol and increase of MDA content (P [0.05). The results of the present study suggest that the seeds of modern white lupine varieties can be included at 250 g/kg in layer diets as an effective substitute for soybean meal, improving fatty acids profile, health lipid indices and oxidative stability of egg yolk.

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