THE METABOLISM OF NITROGEN AND THE DIGESTIBILITY COEFFICIENT AND BIOLOGICAL VALUE OF THE PROTEINS AND NET PROTEIN UTILIZATION IN POOR RICE DIET SUPPLEMENTED WITH METHIONINE-FORTIFIED SOYA FLOUR OR SKIM MILK POWDER

1964 ◽  
Vol 42 (5) ◽  
pp. 641-650 ◽  
Author(s):  
Myna Panemangalore ◽  
H. N. Parthasarathy ◽  
Kantha Joseph ◽  
A. N. Sankaran ◽  
M. Narayana Rao ◽  
...  

The effect of supplementing a poor rice diet commonly used in India with methionine-fortified soya flour or skim milk powder was studied in seven girls aged 8–9 years, using as criteria: the retention of nitrogen, digestibility coefficient, biological value, and net protein utilization of the proteins. The retention of nitrogen on the rice diet was very low (20.3 mg/kg day). Supplementation of the rice diet with soya flour, methionine-fortified soya flour or skim milk powder, so as to provide about 1 g/kg extra protein, made up the protein deficiency in the rice diet and resulted in a significant increase in nitrogen retention (95.6, 112.1, and 113.6 mg/kg day). The retention of nitrogen on the rice diet supplemented with methionine-fortified soya flour (112.1 mg/kg day) or skim milk powder (113.6 mg/kg day) was nearly the same and significantly higher than that (95.6 mg/kg day) observed with a diet supplemented with soya flour. The biological value and net protein utilization of the mixed proteins of rice – skim milk powder diet and rice–methionine-fortified soya flour diet (62.4 and 62.2 and 53.3 and 54.0 respectively) were nearly the same and significantly higher than those (58.3 and 49.9) of rice – soya flour diet. The results show that methionine-fortified soya flour is almost as good as skim milk powder and significantly superior to soya flour as a protein supplement to rice diet.

1964 ◽  
Vol 42 (3) ◽  
pp. 377-384 ◽  
Author(s):  
H. N. Parthasarathy ◽  
T. R. Doraiswamy ◽  
Myna Panemangalore ◽  
M. Narayana Rao ◽  
B. S. Chandrasekhar ◽  
...  

The true digestibility coefficient, biological value, and net available protein of diets based on processed soya flour supplemented with dl-methionine hydroxy analogue (MHA) or dl-methionine (at a level of 1.2 g/16 g N) have been determined in children aged 8–9 years. The mean daily intake of protein by the children on the different diets was maintained at a level of about 1.2 g/kg body weight. Supplementation of soya flour with dl-methionine brought about a marked increase in the biological value and net protein utilization of the proteins. MHA was, however, slightly less effective than dl-methionine in this respect. The biological value and net protein utilization of the different proteins were as follows: soya flour, 63.5 and 53.3; soya flour + MHA, 71.5 and 61.4; soya flour + methionine, 74.9 and 64.7; and skim milk powder, 82.6 and 72.0.


1964 ◽  
Vol 42 (3) ◽  
pp. 385-393 ◽  
Author(s):  
H. N. Parthasarathy ◽  
Kantha Joseph ◽  
V. A. Daniel ◽  
T. R. Doraiswamy ◽  
A. N. Sankaran ◽  
...  

The effect of supplementing a rice diet providing about 1.3–1.4 g protein per kg body weight with lysine, methionine, and threonine individually or together on true digestibility coefficient (DC), biological value (BV), and net protein utilization (NPU) of the proteins has been studied in girls aged 8–9 years. The retention of nitrogen on the rice diet was very low (9.5% of intake in the first series and 8.5% in the second series). The BV and NPU of the proteins of rice diet were 64.1 and 52.9 in the first series and 66.6 and 54.9 in the second series. Supplementation of the rice diet with lysine or methionine or lysine + methionine brought about a significant improvement in N retention (12.6, 12.0, 13.5% of intake) and in the BV (68.3, 66.3, 69.3) and NPU (54.8, 55.7, 55.8). When the rice diet was supplemented with lysine and threonine, a highly significant improvement in the N retention (18.9% of intake) and in the BV (77.4) and NPU (63.4) was observed. Addition of methionine to rice diet containing lysine and threonine resulted in a further improvement in N retention, BV, and NPU of the diets. The net available protein (g/kg body weight) from the different diets were as follows: rice diet, 0.71; rice diet + lysine, 0.76; rice diet + methionine, 0.74; rice diet + lysine + methionine, 0.77; rice diet + lysine + threonine, 0.85; rice diet + lysine + threonine + methionine, 0.91; and skim milk powder diet, 0.96–0.98.


1965 ◽  
Vol 43 (11) ◽  
pp. 1879-1883 ◽  
Author(s):  
M. A. Krishnaswamy ◽  
S. B. Kadkol ◽  
G. D. Revankar

Ensiled fish was prepared from a local variety of freshwater fish (Barbus carnaticus) by fermentation with a pure culture of Streptococcus lactis, commercial lactose being used as a source of fermentable carbohydrate. The fermented material (pH 4.7) was roller dried. The finished product was cream colored and had a somewhat aromatic odor. It had a protein content of about 72%. Total lysine, available lysine, methionine, cystine, and tryptophan of the ensiled fish (expressed as g/16 g N) were 10.1, 8.1, 3.6, 1.1, and 1.2%, respectively. Hygienically, the product, being free from coliforms, enterococci, Salmonella, coagulase-positive staphylococci, and pathogenic anaerobes, was satisfactory. The biological value of the product as determined by protein efficiency ratio (3.3), net protein utilization (82.3%), and net protein ratio (4.2) was not significantly different from that of skim milk powder, which has a protein efficiency ratio of 3.2, net protein utilization of 82.8%, and net protein ratio of 4.9.


1972 ◽  
Vol 52 (3) ◽  
pp. 477-484 ◽  
Author(s):  
A. D. L. GORRILL ◽  
J. W. G. NICHOLSON

The operation of the Willems Polytron® to prepare batches of high-fat (27–35%, air dry basis) milk replacers is described. Most milk replacers were mixed at 40% solids to minimize foaming, and were diluted prior to feeding. The Polytron homogenized fat in liquid mixtures at 55 or 65 C, and partially dispersed insoluble ingredients in soybean, rape-seed, and fish flours. The inclusion of 25% or more of partially delactosed acid whey powder increased the viscosity of the mixtures, and held insoluble ingredients in suspension for several hours. Homogenization of a milk replacer containing 73% skim milk powder and 27% bleachable fancy tallow (3% soybean lecithin added) markedly improved growth (425 vs. 190 g/day), dry matter, nitrogen, and energy digestion (92 vs. 73% for energy) and nitrogen retention (44 vs. 24%) by calves. Calf responses were similar when the liquid diet was homogenized at either 55 or 65 C. Homogenization with 3% soybean lecithin in the tallow increased digestion of dry matter, nitrogen, and energy by chives by 5–6% units (P < 0.2), and Ca by 16% units (P < 0.05) compared with no lecithin. Bleachable fancy or crude tallow and 3% soybean lecithin or 0.2% glyceryl monostearate in the fat gave similar calf responses.


1946 ◽  
Vol 14 (3) ◽  
pp. 403-414 ◽  
Author(s):  
Kathleen M. Henry ◽  
S. J. Rowland

1. A sample of roller-dried skim-milk powder, found to possess the very low biological value of 55.2, supplemented slightly but definitely the proteins of national wheatmeal bread when it supplied 23% of the total nitrogen. No such effect was observed with another sample of milk with a biological value of 71.1 when it supplied 17 % of the total nitrogen, or with a further sample of milk with a biological value of 81.3 when added to bread at a 6 % level supplying about 15 % of the total nitrogen. On the first two occasions the biological value for two different samples of national wheatmeal bread was 48.9 and 55.0 respectively for young rats; for the same rats when mature the values were 55.8 and 64.6. No differences attributable to age were found with milk. Baking did not affect the biological value of the proteins of the milk added to the flour.2. The biological value of a sample of spray.dried skim milk declined from a value of 88.5, observed 18 months after manufacture, to 71.1 some 36 months later. The true digestibility remained unchanged.3. Commercial samples of spray.dried and roller.dried skim milk of good quality, and of over.neutralized and overheated roller.dried skim milk, yielded biological values of 78.6, 80.9, 78.2 and 76.2 respectively and true digestibilities of 90.5, 90.1, 89.4 and 88.8 respectively.


1985 ◽  
Vol 54 (3) ◽  
pp. 727-739 ◽  
Author(s):  
B. O. Eggum ◽  
R. M. Beames ◽  
K. E. Bach Knudsen

1. The present work with growing rats was undertaken to study the effect of protein quality, gastrointestinal microbial activity and the level of nitrogen intake on protein utilization and energy digestibiiity. The experiment involved a total of thirty-six dietary treatments in a 9 x 4 factorial design, with five rats per treatment. The thirty-six diets resulted from nine protein sources. Each diet was composed of a basal N-free mixture plus minerals and vitamins, with N sources added at the expense of the N-free mixture to provide 15.0 gN/kg dry matter (DM) in the first three protein-addition treatments and 30.0 gN/kg DM in the fourth protein-addition treatment. The nine protein sources were soya-bean meal, casein, wheat gluten, skim-milk powder, meat-and-bone meal, wheat bran, barley, wheat and cooked brown beans (Phaseolus vulgaris). The four formulations for each protein source incorporated the protein unsupplemented at 15.0 gN/kg DM, unsupplemented at 30.0 gN/kg DM, or supplemented at 15.0 gN/kg DM with the estimated first-limiting amino acid or the antibiotic Nebacitin.2. With all protein sources, the inclusion of the first-limiting amino acid had no effect on either protein or energy digestibility.3. The microbial activity in the digestive tract affected protein utilization and energy digestibility to a different degree depending primarily on the level and type of dietary fibre. True protein digestibility (TD) of skim-milk powder and brown beans, both rich in easily-fermentable energy, increased from 0.959 to 1.000 and from 0.680 to 0.777 respectively by the addition of Nebacitin. TD of the other protein sources was only marginally affected by the antibiotic treatment. Only with brown beans was the biological value (BV) markedly affected by Nebacitin with an increase from 0.482 to 0.557 by the treatment. Energy digestibility was significantly lower in rats given antibiotic with soya-bean meal, wheat bran, barley, wheat and brown beans.4. The effect of level of N intake on protein utilization was dependent on both protein quality and the fibre concentration of the diet. Protein sources with high BV were more affected than proteins of lower BV. It was concluded that TD is not always independent of dietary protein concentration.


1981 ◽  
Vol 32 (1) ◽  
pp. 67-73 ◽  
Author(s):  
I. G. Partridge

ABSTRACTThirty-six pigs were weaned at 21 days of age and were given diets supplemented with either dicalcium phosphate (DCP) or defluorinated rock phosphate (DFP) from 4 weeks of age until slaughter at 50 kg live weight. From 4 to 9 weeks of age the diets contained either skim milk powder (SKIM) or soya bean meal (SOY) as the main protein supplement. The dietary treatments were arranged in a 2 × 2 factorial design. From 9 weeks of age until slaughter, SOY was the only protein supplement used. Four 7-day measurements of phosphorus, calcium and nitrogen balance were made on each pig. Faecal endogenous P was estimated in two pigs per treatment by the 32P dilution method.There were no significant effects of P supplement on performance or on apparent absorption, true absorption or retention of P. The apparent absorption and retention of Ca were both 0·07 lower with DFP than with DCP in the period from 9 weeks of age to slaughter (P< 0·001).From 4 to 9 weeks of age there was no effect of protein supplement on performance. From 9 weeks of age to slaughter, when all pigs were given SOY, those which had received SKIM in the previous period grew slightly faster (P<0·05); their growth-rate overall, from 4 weeks of age to slaughter, was also significantly better (P <0·05).Pigs given SKIM showed a higher apparent absorption of P (P < 0·001) and higher apparent absorption (P<0·01) and retention (P<0·001) of both Ca and N from 4 to 9 weeks of age. Again, there were carry-over effects in the period from 9 weeks of age to slaughter, although the trends observed in the first period were reversed; pigs previously given SKIM showed significantly lower apparent absorption and retention of both P and Ca, and apparent absorption of N, than those previously given SOY.There were no significant effects of dietary treatments on the ash, Ca or P content of the fourth metacarpal.


1985 ◽  
Vol 54 (2) ◽  
pp. 343-347 ◽  
Author(s):  
Oyus A. Oyeleke ◽  
I. D. Morton ◽  
A. E. Bender

1. A weaning food commonly used in Nigeria was simulated by mixing processed sorghum (Sorghum bicolor) with skim-milk powder (830: 170, w/w).2. Replacing 310 g/kg sorghum with processed cowpeas (Vigna unguiculata) resulted in an increase in protein content from 96 to 113 g/kg and an increase in biological value of the protein from 0.74 to 0.87.3. The two mixtures were compared with an established commercial baby food by a panel of Nigerian mothers and all three foods were found to be equally acceptable.4. Processing the cowpeas by pressure cooking followed by roller-drying reduced the trypsin-inhibitor content to minimum levels.5. It is concluded that the protein content and quality of the weaning mixture popularly used in Nigeria could be improved by partially replacing the sorghum with cowpeas.


2021 ◽  
pp. 106757
Author(s):  
Jianfeng Wu ◽  
Simin Chen ◽  
Teng Wang ◽  
Hao Li ◽  
Ali Sedaghat Doost ◽  
...  

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