RESPIRATION OF STRAIN L CELLS AND OF THEIR VARIANT, LE CELLS

1964 ◽  
Vol 42 (4) ◽  
pp. 513-520 ◽  
Author(s):  
S. Fedoroff ◽  
V. R. Woodford
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Uptake ◽  
Parent Strain ◽  
Carbon Dioxide Production ◽  
Anaerobic Conditions ◽  
L Cells

The oxygen uptake and carbon dioxide production of strain L cells and their variant LE cells were measured by using Warburg techniques with glucose as the sole substrate. The variant LE cells consumed oxygen at a greater rate than did the parent strain L cells. Under anaerobic conditions the situation was reversed, that is, LE cells released less carbon dioxide than did the parent strain L cells.

The Relations Between Yolk and White in the Hen'S Egg

1931 ◽  
Vol 8 (3) ◽  
pp. 319-329
Author(s):  
JOSEPH NEEDHAM ◽  
MARJORY STEPHENSON ◽  
DOROTHY MOYLE NEEDHAM
Keyword(s):  
Carbon Dioxide ◽  
Catalytic Activity ◽  
Lactic Acid ◽  
Carbon Dioxide Production ◽  
Energy Output ◽  
Vitelline Membrane ◽  
Aerobic Respiration ◽  
Anaerobic Conditions ◽  
Hen’S Egg

1. The vitelline membrane of the infertile hen's egg exhibits no dehydrase activity. 2. The vitelline membrane has no measurable aerobic respiration in vitro, nor has the yolk of the infertile egg. This confirms the view that the carbon dioxide production of the intact egg is not the result of any true respiration. 3. When incubated anaerobically in vitro, bacteriologically sterile yolk produces consistently small amounts of lactic acid. 4. This glycolysis is not the result of any catalytic activity of the vitelline membrane, but takes place throughout the substance of the yolk. 5. Under similar conditions, bacteriologically sterile yolk produces small amounts of a substance or substances estimatable as ethyl alcohol. 6. If the yolk suspension is bacterially contaminated, however, lactic acid and alcohol are produced in amounts closely similar to those found by earlier workers on this subject. 7. The heat of glycolysis, under anaerobic conditions, calculated from the amounts of lactic acid experimentally found to be formed, is of the same order as (a) the calculated requirement of the vitelline membrane (Straub), and (b) the observed heat production (Langworthy and Barott). Thus even if the vitelline membrane is capable of using energy to do osmotic work, the yolk is only capable of supplying it by means of its glycolytic mechanism if the whole energy output of the whole yolk can be made available for doing work at the membrane.

scholarly journals GLYCOLYSIS IN RAT PERITONEAL MAST CELLS

1965 ◽  
Vol 25 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Nirmal Chakravarty
Keyword(s):  
Carbon Dioxide ◽  
Mast Cells ◽  
Oxygen Uptake ◽  
Glucose Utilization ◽  
Aerobic Glycolysis ◽  
Carbon Dioxide Production ◽  
Anaerobic Glycolysis ◽  
Glycolytic Activity ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

Glycolytic activity of rat peritoneal mast cells has been measured by the Cartesian ampulla diver technique. The rates of anaerobic glycolysis, expressed as CO2 expelled from a bicarbonate medium, are 1.70 x 10-6 µl and 1.43 x 10-6 µl per cell per hour with and without glucose, respectively. The aerobic glycolysis rate in the presence of glucose, assuming the respiratory quotient to be 1, is 0.93 x 10-6 µl CO2 per cell per hour. It is pointed out that the anaerobic and non-respiratory aerobic carbon dioxide production by mast cells is much higher than the respiratory oxygen uptake reported previously. These values have been interpreted in terms of glucose utilization.

The Minute Ventilation/Carbon Dioxide Production Slope is Prognostically Superior to the Oxygen Uptake Efficiency Slope

2007 ◽  
Vol 13 (6) ◽  
pp. 462-469 ◽  
Author(s):  
Ross Arena ◽  
Jonathan Myers ◽  
Leon Hsu ◽  
Mary Ann Peberdy ◽  
Sherry Pinkstaff ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Uptake ◽  
Minute Ventilation ◽  
Carbon Dioxide Production ◽  
Uptake Efficiency ◽  
Oxygen Uptake Efficiency Slope

Dependence of mitosis and respiration in roots upon oxygen tension

1961 ◽  
Vol 154 (954) ◽  
pp. 109-129 ◽  
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Uptake ◽  
Oxygen Tension ◽  
Oxygen Requirement ◽  
Anaerobic Conditions ◽  
Root Tips ◽  
Carbon Dioxide Output ◽  
Lower Oxygen ◽  
Oxygen Tensions ◽  
The Relationship

Excised pea-root tips were incubated for 4 h in gas mixtures containing 0.00001 to 100% oxygen, in order to determine the effect upon mitosis. Below 0.0005% oxygen, mitosis was completely arrested. Between 0.001 and 0.02% oxygen, cells initially in mitosis completed division, but no more cells started dividing. Between 0.05 and 0.2% oxygen, cells initially in interphase entered division, but did not finish. Above 0.5% oxygen, all cells not prevented from dividing by excision finished division within 4 h. After exposure to 0.05% oxygen for 4 h, an excessive proportion of cells was found in prophase; in 0.1% oxygen an excess of metaphases, and in 0.2% oxygen an excess of telophases resulted. The oxygen uptake and carbon dioxide output of root tips were measured in a range of oxygen tensions and in anaerobic conditions. The relationship between oxygen uptake and oxygen tension was hyperbolic; a half maximum rate of oxygen uptake was obtained at about 10% oxygen. It was concluded that the respiration of root tips was limited by slow diffusion of oxygen through the tissue. From the carbon dioxide output it was estimated that the amount of energy available to isolated root tips under anaerobic conditions was about 1% of that available under aerobic conditions. Possible mechanisms whereby extreme oxygen-lack could arrest mitosis were considered. It was shown that the arrest was not due to abolition of a gross supply of energy. No evidence was obtained as to what other mechanism might be operative. An hypothesis was formulated in an attempt to explain the complicated relationship between mitosis and oxygen tension. It was assumed that the visible phases of mitosis are immediately preceded by a phase with a higher requirement for oxygen than mitosis, and that preceding this is an earlier phase with a lower oxygen requirement than mitosis.

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