FATTY ACID COMPOSITION OF PHOSPHATIDYL ETHANOL-AMINE AND PHOSPHATIDYL SERINE FROM DOG BILE

1964 ◽  
Vol 42 (3) ◽  
pp. 309-316 ◽  
Author(s):  
U. K. Misra ◽  
D. A. Turner

Phosphatidyl ethanolamine and phosphatidyl serine extracted from dog bile have been separated by means of ammonium silicate column chromatography. Concentration of phosphatidyl serine in dog bile is about seven times higher than phosphatidyl ethanolamine. Fatty acid analysis by gas chromatography showed that phosphatidyl ethanolamine contains about 26% palmitic acid, 18% stearic acid, 11% linoleic acid, 2% linolenic acid, 9% arachidonic acid, 3% C22:5 fatty acid, and 6% C22:6 fatty acid. The concentrations of these fatty acids observed in phosphatidyl serine are different; palmitic acid represents about 43%, stearic acid 9%, linoleic acid 24%, linolenic acid a trace amount, and arachidonic acid 5%; C22:5 and C22:6 fatty acids are absent.

2012 ◽  
Vol 66 (2) ◽  
pp. 207-209 ◽  
Author(s):  
Boris Pejin ◽  
Ljubodrag Vujisic ◽  
Marko Sabovljevic ◽  
Vele Tesevic ◽  
Vlatka Vajs

The fatty acid composition of the moss species Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) and Hypnum andoi A.J.E. Sm. (Hypnaceae) collected in winter time were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) as a contribution to their chemistry. Eight fatty acids were identified in the chloroform/methanol extract 1:1 of A. undulatum (linoleic acid 26.80%, palmitic acid 22.17%, ?-linolenic acid 20.50%, oleic acid 18.49%, arachidonic acid 6.21%, stearic acid 3.34%, cis-5,8,11,14,17-eicosapentaenoic acid 1.52% and behenic acid 1.01%), while six fatty acids were found in the same type of extract of H. andoi (palmitic acid 63.48%, erucic acid 12.38%, stearic acid 8.08%, behenic acid 6.26%, lignoceric acid 5.16% and arachidic acid 4.64%). According to this study, the moss A. undulatum can be considered as a good source of both essential fatty acids for humans (linoleic acid and ?-linolenic acid) during the winter.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 453c-453 ◽  
Author(s):  
Jenith Cyril ◽  
R.R. Duncan ◽  
W.V. Baird

Three genotypes of seashore paspalum, `PI 299042', `Adalayd', and `PI 509018-1' considered to be cold-sensitive, intermediately cold-tolerant and cold-tolerant, respectively, were analyzed to investigate the biochemical basis of cold tolerance. The cultivars were acclimated to 8/4 °C day/night temperatures and rhizomes nodes and crowns were harvested at 7-day intervals over the 4-week experiment. Total lipid was extracted from these tissues, and the fatty acids present in the lipid fraction were identified by gas chromatography. Palmitic acid, stearic acid, linoleic acid and linolenic acid were the major fatty acids present. In cold acclimated tissues, the level of palmitic acid and stearic acid did not change significantly during the treatment period. There was a decrease in the level of linoleic acid by the second week of cold treatment. The amount of linolenic acid increased significantly during the second week of cold treatment corresponding to the decrease in linoleic acid. The change in the amount was significantly greater in `PI 509018-1' than in `Adalayd' or `PI 299042'. These results are similar to what was found for cultivars of bermudagrass that differ in their cold-tolerance phenotypes. Desaturases are enzymes involved in introducing the double bonds into the fatty acid chains. Research is underway to characterize and clone the genes encoding the Ω3, Ω6, and Ω9 desaturases, which may have an important role in affecting the cold tolerance by altering the degree of membrane lipid fatty acid saturation.


1969 ◽  
Vol 36 (3) ◽  
pp. 393-398 ◽  
Author(s):  
R. C. Noble ◽  
W. Steele ◽  
J. H. Moore

SummaryCows were given either a low-fat concentrate mixture or a concentrate mixture that contained 5 or 10% ‘stearic acid’ (85% pure) or 10% ‘palmitic acid’ (85% pure). The concentrate mixtures were given with a high roughage diet that supplied 4·4 kg of hay and 2·7 kg of sugar-beet pulp/day. Blood samples were taken from the cows on each dietary treatment and the plasma phospholipids were analysed.Phosphatidyl choline accounted for 70% of the plasma phospholipids when the cows were given the low-fat diet and about 86% of the plasma phospholipids when the cows were given the diets supplemented with the fatty acids. The inclusion of the fatty acids in the concentrate mixtures decreased the relative proportions of the plasma phosphatidyl ethanolamine, sphingomyelin and lysophosphatidyl choline but the relative proportion of the plasma phosphatidyl serine remained unchanged.When the diet was supplemented with stearic acid the concentrations of 18:0 and 16:0 in the phosphatidyl choline were unaltered but the concentration of 18:1 was increased and the concentration of 18:2 was decreased. When the diet was supplemented with palmitic acid the concentrations of 16:0 and 18:1 in the phosphatidyl choline were increased and the concentrations of 18:0 and 18:2 were decreased. In contrast, the inclusion of stearic acid in the diet increased the concentration of 18:0 in the phosphatidyl serine and decreased the concentration of 16:0; the concentrations of 18:1 and 18:2 were unchanged. The fatty acid composition of the plasma phosphatidyl ethanolamine was unaffected by dietary treatment. The effects of diet on the fatty acid compositions of the phosphatidyl choline and lysophosphatidyl choline were similar. Supplementation of the diet with stearic acid increased the concentrations of 18:0 and 18:1 in the plasma sphingomyelin and decreased the concentrations of 16:0 and 18:2. The addition of palmitic acid to the diet increased the concentration of 16:0 in the sphingomyelin but it decreased the concentrations of the other constituent fatty acids.


Author(s):  
Vilcacundo E ◽  
Alvarez M ◽  
Silva M ◽  
Carpio C ◽  
Morales D ◽  
...  

 Objective: The aim of this study was to determine the fatty acids composition in a tocte seeds oil (Juglans neotropica Diels) sample cultivated in Ecuador.Methods: Tocte oil was obtained from tocte seeds using the cold pressing method. Fatty acids analysis was carried out using the gas chromatography method with a mass selective detector (GC/MSD) and using the database Library NIST14.L to identify the compounds.Results: Methyl esters fatty acids were identified from tocte (J. neotropica Diels) walnut using the GC–MS analytical method. The total lipid content of tocte walnuts seeds of plants cultivated in Ecuador was of 49.01% of the total lipid content on fresh weight. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GC–MS. Tocte walnut presents 5.05% of palmitic acid, 2.26% of stearic acid, 19.50% of oleic acid, 65.81% of linoleic acid, and 2.79% linolenic acid of the total content of fatty acids in tocte oil. Fatty acids content reported in this study were similar to the data reported for other walnuts seeds.Conclusions: Tocte seeds are a good source of monounsaturated and polyunsaturated fatty acids. Tocte oil content oleic acid and with a good content of ɷ6 α-linoleic and ɷ3 α-linolenic. Tocte walnut can help reduce risk cardiovascular diseases in Ecuador for their good composition of fatty acids.


Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 82
Author(s):  
M. H. Rachmawati ◽  
H. Soetjipto ◽  
A. Ign A. Ign. Kristijanto

Overripe tempe is a food product that used by peoples in Indonesia as a food seasoning. So far, overripe tempe received less attention than fresh tempe and research of overripe tempe is rarely done. The objective of the study is to identify the fatty acid compounds of the  fifth day fermentation overripe tempe oil before and after purification . The overripe tempe oil of fifth day fermentation was extracted with soxhletation method using n – hexane solvent, then it was purified. The various fatty acids  of overripe tempe oil were analyzed by GC – MS. The purification process was done by using H3PO4 0,2% and NaOH 0,1N. The result of the study showed that before purification the oil  was composed of eight compounds  are palmitic acid (13,33%),  linoleic acid (77,57%), stearic acid (6,15%), and the five chemical components, Dasycarpidan – 1 - methanol, acetate ,  oleic acid, 9 - Octadecenamide ,Cholestane - 3, 7, 12, 25 - tetrol, tetraacetate, (3?, 5?, 7?, 12?) and  6, 7 – Epoxypregn – 4 – ene -9, 11, 18- triol - 3, 20 - dione, 11, 18 – diacetate have percentage of areas less than 3%. After purification the oil  was composed of palmitic acid (12,38% ), linoleic acid (80,35 %), stearic acid (5,84%), and 17 – Octadecynoic acid (1,42 %) .


2019 ◽  
Vol 54 (4) ◽  
pp. 367-374
Author(s):  
MO Aremu ◽  
AA Waziri ◽  
FJ Faleye ◽  
AM Magomya ◽  
UC Okpaegbe

There are several underexploited plant seeds or fruits in Nigeria with little information about their chemical composition. To this end a comprehensive study on fatty acid, phospholipids and phytosterols composition of bitter melon (Momordica charaantia) fruit and ebony tree (Diospyros mespiliformis) fruit pulp were determined using standard analytical techniques. The most concentrated fatty acid (%) was linoleic acid in Momordica charantia fruit (45.47) and 44.82 in Diospyros mespiliformis fruit pulp. The increasing order of the concentrated fatty acids in Momordica charantia fruit were: linolenic acid (2.38) < stearic acid (7.52) < oleic acid (20.18) < palmitic acid (23.64) < linoleic acid (45.47) while that of Diospyros mespiliformis fruit pulp were: linolenic acid (5.73) < stearic acid (8.62) < oleic acid (18.95) < palmitic acid (20.88) < linoleic acid (44.82). Arachidonic, arachidic, palmitoleic, margaric, behenic, erucic, lignoceric, myristic, lauric, capric and caprylic acids were present in small quantities with none of them recording up to 1.0% in both of the two samples. The results also showed low concentration of monounsaturated fatty acids (MUFA) (20.41%) in Momordica charantia fruit and 19.13% in Diospyros mespiliformis fruit pulp, and values of polyunsaturated fatty acid (PUFA) were 2.44 and 5.78% for the two samples, respectively. The respective phospholipids composition showed a highest concentration of phosphatidylcholine in Momordica charantia and Diospyros mespiliformis (100.31and 88.12 mg/100 g) while lysophosphatidylcholine and phosphatidic acid were the least concentrate values of 12.62 and 14.52 mg/100 g in Momordicacharantia and Diospyros mespiliformis, respectively. The concentrations of phytosterols were of low values except in sitosterol with values of 153.28 and 119.46 mg/100 g in Momordica charantia and Diospyros mespiliformis, respectively. This study provides an informative lipid profile that will serve as a basis for further chemical investigations and nutritional evaluation of Momordica charantia fruit and Diospyros mespiliformis fruit pulp. Bangladesh J. Sci. Ind. Res.54(4), 367-374, 2019


1980 ◽  
Vol 191 (2) ◽  
pp. 637-643 ◽  
Author(s):  
William W. Christie ◽  
Margaret L. Hunter

The effects of inclusion of different fatty acids in the medium on the rate of esterification of palmitic acid and its stereospecific distribution among the three positions of the triacyl-sn-glycerols by preparations of rat adipocytes in vitro have been determined. Myristic acid, stearic acid, oleic acid and linoleic acid were used as diluents and the concentration of the combined unesterified fatty acids in the medium was held constant; only the proportion of palmitic acid was varied. The amount of palmitic acid esterified was always linearly related to its relative concentration in the medium and was not significantly affected by the nature of the diluent fatty acid chosen. Constant relative proportions were recovered in triacylglycerols and in intermediates in each instance. The amount of palmitic acid esterified to each of the positions of the triacyl-sn-glycerols was linearly dependent on the relative proportion in the medium but the nature of the relationship was markedly influenced by which fatty acid was present. When stearic acid was present, simple relationships were found over the whole range tested. When either myristic acid, oleic acid or linoleic acid was present, abrupt changes in the manner of esterification of palmitic acid were observed in position sn-1 when the relative concentrations of palmitic acid and the diluent reached critical values, which differed with each fatty acid. In position sn-2 when oleic acid or linoleic acid was present, a similar change was observed, and in position sn-3 it was obtained with myristic acid as diluent. The results are discussed in terms of changes in the relative affinities of the acyltransferases for palmitic acid. Palmitic acid was esterified into various molecular species in proportions that indicated acylation with non-correlative specificity at higher relative concentrations but not at lower.


1990 ◽  
Vol 115 (5) ◽  
pp. 803-808 ◽  
Author(s):  
Shiow Y. Wang ◽  
Miklos Faust

The changes of membrane lipids in apple (Malus domestics Borkh. cv. Delicious) auxillary and terminal buds from August to April were determined. The predominant lipids were monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE). An increase in membrane polar lipids was associated with budbreak and bud growth from August to April. Linolenic acid was the predominant fatty acid in MGDG, DGDG, and PC, while linoleic acid was predominant in PE. Phosphatidylglycerol (PG) and phosphatidylinositol (PI) contained a high amount of palmitic acid. The ratio of (18:2 + 18:3) to 18:1 fatty acids in galactolipids in apple buds increased from August to April. ß-Sitosterol and sitosteryl ester were the predominant sterols in apple buds. An increase in sitosterol, a decrease in sitosteryl ester, and a decline in the ratio of free sterols to phospholipids occurred during budbreak in spring. A decrease in sitosterol was associated with bud expansion in spring.


Author(s):  
Deniz Altuntaş ◽  
Hakan Allı ◽  
Erhan Kaplaner ◽  
Mehmet Öztürk

Human being have been consumed mushrooms due to their aroma and flavour. The macro-nutritional properties such as ash, protein fat, carbohydrate and energy and fatty acid ingredients of Lactarius deliciosus (L.) Gray, Lactarius deterrimus Gröger, Lactarius salmonicolor R. Heim & Leclair and Lactarius semisanguifluus R. Heim & Leclair were studied. The results indicate that the moisture was between in the range of 86.8-91.1%, while the ash 5.1-9.2%, and the protein 9.4-19.0%, and the fat 0.6-1.1%, and the carbohydrate 71.8-83.9, and the energy calculated between 372.1-382.6 kcal/100 g dry weights. The major fatty acids were determines as stearic acid, oleic acid, linoleic acid and palmitic acid in the range of 6.68-39.41%, 26.94-47.12%, 9.78-23.85% and 9.7-14.43% respectively.


2013 ◽  
pp. 45-50
Author(s):  
Ágnes Süli ◽  
Béla Béri ◽  
János Csapó ◽  
Éva Vargáné Visi

In the last decades many researches were made to change the animal product food’s composition. The production of better fat-compound milk and dairy products became a goal in the name of health conscious nutrition. These researches were motivated by the non adequate milk fat’s fatty acid composition. There have been made researches in order to modify the milk’s fatty acids’ composition to reach the expectations of functional foods. With the optimal supplement of the feed can be increased the proportion of the polyunsaturated fatty acids and can decreased the saturated fatty acids. Row fat content of milk was not decreasing in the course of examination neither of the cold extruded linseed nor the whole linseed supplement as opposed to observations experienced by other authors. In case of monounsaturated and polyunsaturated fatty acids when supplementing with cold extruded linseed the most significant change was observable in the concentration of the elaidic acid, oleic acid, linoleic acid, alfa-linolenic acid, conjugated linoleic acid. In case of saturated fatty acids the quantity of palmitic acid and myristic acid lowered considerably. When observating the feeding with whole linseed the concentration of many fatty acids from the milkfat of saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, alfa-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers as well. The producing of milk with favourable fatty acid content from human health point of view can give scope propagate the products of animal origin.  


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