PROPERTIES OF HUMAN PLASMIN

Edward Ronwin

doi:10.1139/o62-008

PROPERTIES OF HUMAN PLASMIN

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y62-008 ◽

1962 ◽

Vol 40 (1) ◽

pp. 49-55 ◽

Cited By ~ 1

Author(s):

Edward Ronwin

Keyword(s):

Ionic Strength ◽

Similar Data ◽

Glycerol Molecule ◽

Substrate Specificities ◽

Ion Activation ◽

Peculiar Property ◽

Related Compounds

Several aspects of the properties and preparation of human plasmin are considered. It appears that glycerol activation of the proenzyme is a peculiar property dependent in part on the structure of the glycerol molecule since closely related compounds are without effect. Observations on the influence of ionic strength, on ion activation and inhibition of the enzyme, on substrate specificities, and on preparative procedures are recorded and comparisons with similar data on related bovine enzymes are made.

Download Full-text

Réactions dans le sulfolane. V. Ions trihalogénures

Canadian Journal of Chemistry ◽

10.1139/v70-108 ◽

1970 ◽

Vol 48 (4) ◽

pp. 674-679 ◽

Cited By ~ 3

Author(s):

M. Deneux ◽

R. L. Benoit

Keyword(s):

Ionic Strength ◽

Stability Constants ◽

Similar Data ◽

The Stability

Equilibria between I2 and I−, Br−, Cl− in sulfolane were studied by potentiometry and spectrophotometry at 30 °C. The stabilities of Br3− and Cl3− were also determined. The stability constants at nil ionic strength were calculated by means of a program: [Formula: see text], 7.23, and 7.23 for Y = I, Br, and Cl; [Formula: see text], 7.33, and 4.11 for X = I, Br, and Cl. The disproportionation equilibria [Formula: see text] were characterized by log Kd = −1.55(Y = Br) and −0.64 (Y = Cl). The stabilities and spectra of the trihalide ions in sulfolane are compared with similar data for other solvents.

Download Full-text

The ionic strength dependence of chromatin folding

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081966 ◽

1978 ◽

Vol 36 (2) ◽

pp. 220-221

Author(s):

F. Thoma ◽

TH. Koller

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Ionic Strength ◽

Specimen Preparation ◽

Preparation Technique ◽

Carbon Supports ◽

Ionic Strength Dependence ◽

Chromatin Folding ◽

Strength Dependence ◽

Preparation Techniques

Under a variety of electron microscope specimen preparation techniques different forms of chromatin appearance can be distinguished: beads-on-a-string, a 100 Å nucleofilament, a 250 Å fiber and a compact 300 to 500 Å fiber.Using a standardized specimen preparation technique we wanted to find out whether there is any relation between these different forms of chromatin or not. We show that with increasing ionic strength a chromatin fiber consisting of a row of nucleo- somes progressively folds up into a solenoid-like structure with a diameter of about 300 Å.For the preparation of chromatin for electron microscopy the avoidance of stretching artifacts during adsorption to the carbon supports is of utmost importance. The samples are fixed with 0.1% glutaraldehyde at 4°C for at least 12 hrs. The material was usually examined between 24 and 48 hrs after the onset of fixation.

Download Full-text

Conformational Transitions in Escherichia coli Ribosomal RNAs as Visualized by Dedicated STEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102961 ◽

1988 ◽

Vol 46 ◽

pp. 176-177

Author(s):

J.S. Wall ◽

V. Maridiyan ◽

S. Tumminia ◽

J. Hairifeld ◽

M. Boublik

Keyword(s):

Ionic Strength ◽

Three Dimensional ◽

Conformational Transitions ◽

Dark Field ◽

Dimensional Structure ◽

Ribosomal Rnas ◽

Field Mode ◽

Freeze Dried ◽

High Resolution Images ◽

Low Ionic Strength

The high contrast in the dark-field mode of dedicated STEM, specimen deposition by the wet film technique and low radiation dose (1 e/Å2) at -160°C make it possible to obtain high resolution images of unstained freeze-dried macromolecules with minimal structural distortion. Since the image intensity is directly related to the local projected mass of the specimen it became feasible to determine the molecular mass and mass distribution within individual macromolecules and from these data to calculate the linear density (M/L) and the radii of gyration.2 This parameter (RQ), reflecting the three-dimensional structure of the macromolecular particles in solution, has been applied to monitor the conformational transitions in E. coli 16S and 23S ribosomal RNAs in solutions of various ionic strength.In spite of the differences in mass (550 kD and 1050 kD, respectively), both 16S and 23S RNA appear equally sensitive to changes in buffer conditions. In deionized water or conditions of extremely low ionic strength both appear as filamentous structures (Fig. la and 2a, respectively) possessing a major backbone with protruding branches which are more frequent and more complex in 23S RNA (Fig. 2a).

Download Full-text

The three-dimensional structure of frozen-hydrated left- and right-handed forms of bacterial flagellar filaments from an identical serotype

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143110 ◽

1986 ◽

Vol 44 ◽

pp. 298-299

Author(s):

S. Trachtenberg ◽

D. J. DeRosier

Keyword(s):

Ionic Strength ◽

Basal Body ◽

Three Dimensional ◽

Dimensional Structure ◽

Protein Species ◽

Liquid Environment ◽

Bacterial Flagellum ◽

Left And Right ◽

Rotary Motor ◽

Helical Parameters

The bacterial cell is propelled through the liquid environment by means of one or more rotating flagella. The bacterial flagellum is composed of a basal body (rotary motor), hook (universal coupler), and filament (propellor). The filament is a rigid helical assembly of only one protein species — flagellin. The filament can adopt different morphologies and change, reversibly, its helical parameters (pitch and hand) as a function of mechanical stress and chemical changes (pH, ionic strength) in the environment.

Download Full-text

Ultrastructure of the basal body apparatus in epidermal cells of a sponge larva (Aplysilla SP: Demospongiae)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012504x ◽

1992 ◽

Vol 50 (1) ◽

pp. 928-929

Author(s):

R.L. Pinto ◽

R.M. Woollacott

Keyword(s):

Sodium Bicarbonate ◽

Epoxy Resin ◽

Basal Body ◽

Phosphate Buffer ◽

Similar Data ◽

Basal Apparatus ◽

Striated Rootlet ◽

The Common ◽

Basal Foot ◽

Sexually Mature

The basal body and its associated rootlet are the organelles responsible for anchoring the flagellum or cilium in the cytoplasm. Structurally, the common denominators of the basal apparatus are the basal body, a basal foot from which microtubules or microfilaments emanate, and a striated rootlet. A study of the basal apparatus from cells of the epidermis of a sponge larva was initiated to provide a comparison with similar data on adult sponges.Sexually mature colonies of Aplysillasp were collected from Keehi Lagoon Marina, Honolulu, Hawaii. Larvae were fixed in 2.5% glutaraldehyde and 0.14 M NaCl in 0.2 M Millonig’s phosphate buffer (pH 7.4). Specimens were postfixed in 1% OsO4 in 1.25% sodium bicarbonate (pH 7.2) and embedded in epoxy resin. The larva ofAplysilla sp was previously described (as Dendrilla cactus) based on live observations and SEM by Woollacott and Hadfield.

Download Full-text

Voice Therapy With Seniors: Attendance, Outcomes, and Associated Patient Characteristics

Perspectives of the ASHA Special Interest Groups ◽

10.1044/2019_pers-sig3-2019-0004 ◽

2019 ◽

Vol 4 (5) ◽

pp. 814-824 ◽

Cited By ~ 1

Author(s):

Bonnie E. Smith ◽

Ruth Huntley Bahr ◽

Hector N. Hernandez

Keyword(s):

Outcome Data ◽

Voice Therapy ◽

Similar Data ◽

Success Rates ◽

Reflux Symptom Index ◽

Voice Change ◽

Related Factors ◽

Symptom Index ◽

Younger Patients ◽

Unsuccessful Treatment

Purpose The purpose of this study was to determine the attendance and success rates for seniors in voice therapy, identify any contributing patient-related factors, and compare results to existing findings for younger patients. Method This retrospective study included information from the voice records of 50 seniors seen by the same speech-language pathologist in a private practice. Analysis of attendance and outcome data divided participants into 6 groups. Outcomes for Groups 1–3 (64% of patients) were considered successful (positive voice change), while outcomes for Groups 4–6 (36% of patients) were considered unsuccessful. These data were compared to similar data collected for younger adults in a previous study. Results The attendance and success rates for seniors in this study were higher than those previously reported for younger patients. Further consideration of patient factors revealed that reports of increased stress, Reflux Symptom Index scores > 13, and higher Voice Handicap Index functional subscale scores were significant in distinguishing between patients in the successful and unsuccessful treatment outcome groups. Conclusions The relatively high attendance and success rates among this sample of seniors suggest the desire to achieve voice improvement does not diminish with age, and chances for success in voice therapy among nonfrail seniors may be greater than for younger patients.

Download Full-text