THE METABOLISM OF INOSITOL IN SALMON: II. THE ROLE OF NUCLEOTIDES IN RELATION TO THE METABOLISM OF 2-C14-MYOINOSITOL IN COHO LIVER

1960 ◽  
Vol 38 (10) ◽  
pp. 1177-1183 ◽  
Author(s):  
H. Tsuyuki ◽  
D. R. Idler
Keyword(s):  
Uronic Acid ◽  
Glucuronic Acid ◽  
Acid Fraction ◽  
Biochemical Reactions ◽  
Entire Spectrum ◽  
Sugar Nucleotide ◽  
Measurable Activity

Though radioactivity is distributed throughout the entire spectrum of coho liver nucleotide fractions, the UDP-uronic acid fraction was found to be one of the more active. Most of the radioactivity was present in the glucuronic acid and glucuronolactone formed when UDP-uronic acid was hydrolyzed with acid. UDP-glucose, UDP-galactose, and UDP-N-acetylhexosamine did not contain any measurable activity. In C14-coho II another major concentration of activity was found in fraction A5, which has not yet been investigated. These results are discussed in relation to the known biochemical reactions of inositol and sugar nucleotide interconversions in plants.

THE METABOLISM OF INOSITOL IN SALMON: II. THE ROLE OF NUCLEOTIDES IN RELATION TO THE METABOLISM OF 2-C14-MYOINOSITOL IN COHO LIVER

1960 ◽  
Vol 38 (1) ◽  
pp. 1177-1183 ◽  
Author(s):  
H. Tsuyuki ◽  
D. R. Idler
Keyword(s):  
Uronic Acid ◽  
Glucuronic Acid ◽  
Acid Fraction ◽  
Biochemical Reactions ◽  
Entire Spectrum ◽  
Sugar Nucleotide ◽  
Measurable Activity

Though radioactivity is distributed throughout the entire spectrum of coho liver nucleotide fractions, the UDP-uronic acid fraction was found to be one of the more active. Most of the radioactivity was present in the glucuronic acid and glucuronolactone formed when UDP-uronic acid was hydrolyzed with acid. UDP-glucose, UDP-galactose, and UDP-N-acetylhexosamine did not contain any measurable activity. In C14-coho II another major concentration of activity was found in fraction A5, which has not yet been investigated. These results are discussed in relation to the known biochemical reactions of inositol and sugar nucleotide interconversions in plants.

A uronic acid isomerase in Flavobacterium heparinum

1970 ◽  
Vol 48 (2) ◽  
pp. 164-169 ◽  
Author(s):  
James C. Karapally ◽  
Carl P. Dietrich
Keyword(s):  
Hyaluronic Acid ◽  
Equilibrium Point ◽  
Galacturonic Acid ◽  
Uronic Acid ◽  
Glucuronic Acid ◽  
Chondroitin Sulfates ◽  
Isolation And Characterization ◽  
Flavobacterium Heparinum

The isolation and characterization of a uronic acid isomerase from F. heparinum is described. The enzyme converts glucuronic acid and galacturonic acid to fructuronic acid and tagaturonic acid. The equilibrium point of the reaction is affected by buffers. In the presence of borate, 75% of glucuronic acid is converted to fructuronic acid. In the presence of phosphate, the equilibrium is reached when 31% of glucuronic acid is converted to fructuronic acid. Conversely, fructuronic acid and tagaturonic acid are converted to glucuronic acid and galacturonic acid, respectively. Monosaccharides derived from heparin and chondroitin sulfates do not affect the activity of the isomerase, in contrast to the monosaccharides from hyaluronic acid which have marked inhibitory (or diluting) activity upon the enzyme. The role of this enzyme in the metabolism of mucopolysaccharides is discussed.

Photosynthetic activity as the basis of potato productivity when using phytohormones

2020 ◽  
Author(s):  
A.V. Shitikova ◽  
◽  
A.A. Abiala
Keyword(s):  
Photosynthetic Activity ◽  
Moscow Region ◽  
Metabolic Processes ◽  
Biochemical Reactions ◽  
Podzolic Soils ◽  
Increase In Productivity

The results of studies on the role of growth biostimulants in the exogenous regulation of potato productivity on sod-podzolic soils of the Moscow region are presented.Studies have established the specificity of the action of phytohormones.The stimulating effect of the drugs manifested itself in the intensification of metabolic processes, changing the direction of biochemical reactions, which led to an increase in productivity.

Binding of lead and copper(II) cations to hemicelluloses of rye bran

1986 ◽  
Vol 51 (10) ◽  
pp. 2250-2258 ◽  
Author(s):  
Rudolf Kohn ◽  
Zdena Hromádková ◽  
Anna Ebringerová
Keyword(s):  
Uronic Acid ◽  
Equilibrium Solution ◽  
Glucuronic Acid ◽  
The Other ◽  
Carboxyl Groups ◽  
Stoichiometric Ratio ◽  
Molar Ratios ◽  
Free Ions ◽  
Uronic Acid Content ◽  
The Relationship

Several fractions of acid hemicelluloses isolated from rye bran were characterized by molar ratios of saccharides (D-Xyl, L-Ara, D-Glc, D-Gal) and 4-O-methyl-D-glucuronic acid and protein content. Binding of Pb2+ and Cu2+ ions to these acid polysaccharides was considered according to function (M)b = f([M2+]f), expressing the relationship between the amount of metal (M)b bound to 1 g of the substance and the concentration of free ions [M2+]f in the equilibrium solution and according to the association degree β of these cations with carboxyl groups of uronic acid at a stoichiometric ratio of both components in the system under investigation. Acid hemicelluloses contained only a very small portion of uronic acid ((COOH) 0.05-0.18 mmol g-1); the model polysaccharide, 4-O-methyl-D-glucurono-D-xylan of beech, was substantially richer in uronic acid content ((COOH) 0.73 mmol g-1). Consequently, the amount of lead and copper bound to acid hemicelluloses is very small ((M)b 0.017-0.025 mmol g-1) at [M2+]f = 0.10 mmol l-1. On the other hand, much greater amount of cations ((M)f 0.09-0.10 mmol g-1) was bound to the glucuronoxylan. The association degree β was like with the majority of samples (β = 0.31-0.38). The amount of lead and copper(II) bound to acid hemicelluloses from rye bran is several times lower than that bound to dietary fiber isolated from vegetables (cabbage, carrot), rich in pectic substances.

scholarly journals Structural studies on heparan sulphate from human lung fibroblasts. Characterization of oligosaccharides obtained by selective periodate oxidation of d-glucuronic acid residues followed by scission in alkali

1980 ◽  
Vol 191 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Ingrid Sjöberg ◽  
Lars-Ȧke Fransson
Keyword(s):  
Uronic Acid ◽  
Glucuronic Acid ◽  
Human Lung ◽  
General Structure ◽  
Heparan Sulphate ◽  
Periodate Oxidation ◽  
Exchange Chromatography ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts ◽  
Iduronic Acid

1. 3H- and 35S-labelled heparan sulphate was isolated from monolayers of human lung fibroblasts and subjected to degradations by (a) deaminative cleavage and (b) periodate oxidation/alkaline elimination. Fragments were resolved by gel- and ion-exchange-chromatography. 2. Deaminative cleavage of the radioactive glycan afforded mainly disaccharides with a low content of ester-sulphate and free sulphate, indicating that a large part (approx. 80%) of the repeating units consisted of uronosyl-glucosamine-N-sulphate. Blocks of non-sulphated [glucuronosyl-N-acetyl glucosamine] repeats (3–4 consecutive units) accounted for the remainder of the chains. 3. By selective oxidation of glucuronic acid residues associated with N-acetylglucosamine, followed by scission in alkali, the radioactive glycan was degraded into a series of fragments. The glucuronosyl-N-acetylglucosamine-containing block regions yielded a compound N-acetylglucosamine–R, where R is the remnant of an oxidized and degraded glucuronic acid. Periodate-insensitive uronic acid residues were recovered in saccharides of the general structure glucosamine–(uronic acid–glucosamine)n–R. 4. Further degradations of these saccharides via deaminative cleavage and re-oxidations with periodate revealed that iduronic acid may be located in sequences such as glucosamine-N-sulphate→iduronic acid→N-acetylglucosamine. Occasionally the iduronic acid was sulphated. Blocks of iduronic acid-containing repeats may contain up to five consecutive units. Alternating arrangements of iduronic acid- and glucuronic acid-containing repeats were also observed. 5. 3H- and 35S-labelled heparan sulphates from sequential extracts of fibroblasts (medium, EDTA, trypsin digest, dithiothreitol extract, cell-soluble and cell-insoluble material) afforded similar profiles after both periodate oxidation/alkaline elimination and deaminative cleavage.

HUMUS FRACTION RATIOS AS A MEANS OF DISCRIMINATING BETWEEN HORIZON TYPES

1980 ◽  
Vol 60 (2) ◽  
pp. 219-229 ◽  
Author(s):  
L. E. LOWE
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Carbon Content ◽  
Fulvic Acid ◽  
Soil Classification ◽  
Acid Fraction ◽  
Humus Fraction ◽  
Wide Range ◽  
Fraction Distribution

Humus fraction distribution in a wide range of horizon samples was examined by measuring carbon content in humic acid (Ch), in fulvic acid (Cf) and in the strongly colored polyphenolic component of the fulvic acid fraction (Ca). Fraction distribution was described by the ratios Ch/Cf and Ca/Cf. It was concluded that humus fraction ratios were related to horizon types as used in the Canadian System of Soil Classification, and were effective in discriminating between certain horizon types, particularly between Luvisolic Bt and Podzolic Bf. The results also suggested that humus fraction ratios may be effective in separating distinct sub-populations within Ah horizons and Bf horizons in general, based on qualitative differences in organic matter present. Aspects of the role of humus fractions in soil genesis are discussed.

Being Able to Survive and Thrive

2019 ◽  
pp. 25-46
Author(s):  
David L. Blustein
Keyword(s):  
Time Perspective ◽  
Human Experience ◽  
Financial Security ◽  
Comprehensive Review ◽  
Entire Spectrum ◽  
Boston College ◽  
The World ◽  
Work Volition ◽  
Psychology Of Working

This chapter presents a comprehensive review of the ways in which the needs for survival and power intersect with working. Beginning with an overview of Maslow’s need hierarchy (which indicates the need for survival is fundamental to our existence) and the psychology-of-working framework, vignettes from the participants from the Boston College Working Project provide an in-depth perspective about the complex ways that striving for survival intersects with relationships, financial security, and thriving. The chapter concludes with a discussion of the role of time perspective and work volition in relation to the need to survive. The chapter makes clear that the drive for survival is an essential aspect of being alive in the world. Creating opportunities for people to meet this integral aspect of human experience, naturally, is a challenge that requires the best of our inner spirits and a commitment to nurturing the needs of the entire spectrum of people in our communities.

Role of conjugation and red blood cells for inactivation of circulating normetanephrine

1984 ◽  
Vol 247 (1) ◽  
pp. R208-R211
Author(s):  
S. Yoneda ◽  
N. Alexander ◽  
N. D. Vlachakis ◽  
R. F. Maronde
Keyword(s):  
Immobilization Stress ◽  
Glucuronic Acid ◽  
Simultaneous Increase ◽  
Sympathoadrenal System ◽  
Methyltransferase Activity ◽  
Substrate Saturation ◽  
The Mean ◽  
Catechol Methyltransferase ◽  
Mean Concentration

Plasma and red blood cell (RBC) concentrations of normetanephrine (NMN), in both free and glucuronide-conjugated forms, were measured before, during, and after forced immobilization, an intense stressor of the sympathoadrenal system of rats. In this study NMN glucuronide was deconjugated by enzymatic hydrolysis; free and total NMN were assayed by radioenzymatic, thin-layer chromatographic procedures. In plasma, free NMN and NMN glucuronide are 777 +/- 99 and 792 +/- 74 pg/ml, respectively, when rats are at rest. Both free NMN and NMN glucuronide increased about 200% after 15 min of stress; in absolute amounts, increases were equivalent to that of the simultaneous increase in norepinephrine (NE). At 2 h of stress, NMN glucuronide, but not free NMN, increased further and significantly. The mean concentration of RBC-free NMN is about 50 times higher than that of plasma-free NMN, and it did not change significantly during stress; RBCs do not contain conjugated NMN. RBC NMN levels showed a strong correlation with RBC catechol methyltransferase activity. The latter seems to operate under conditions of substrate saturation; an acute release of NE leads to temporary storage of NE in RBCs but not conversion to NMN. The results indicate that conjugation of NMN with glucuronic acid is an important route for inactivation of plasma NMN formed during forced immobilization stress, whereas free NMN does not accumulate in RBCs during stress.

scholarly journals Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis

2016 ◽  
Vol 9 (8) ◽  
pp. 1119-1131 ◽  
Author(s):  
Beiqing Kuang ◽  
Xianhai Zhao ◽  
Chun Zhou ◽  
Wei Zeng ◽  
Junli Ren ◽  
...  
Keyword(s):  
Glucuronic Acid ◽  
Acid Decarboxylase ◽  
Xylan Biosynthesis

THE ACID-SOLUBLE NUCLEOTIDES OF SALMON LIVER

1958 ◽  
Vol 36 (5) ◽  
pp. 465-473 ◽  
Author(s):  
H. Tsuyuki ◽  
Violet M. Chang ◽  
D. R. Idler
Keyword(s):  
Low Temperature ◽  
Anion Exchange ◽  
Rat Liver ◽  
Glucuronic Acid ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Uridine Diphosphate ◽  
Carbohydrate Storage ◽  
Diphosphate Glucose

The acid-soluble nucleotides of spring salmon liver have been separated by anion-exchange chromatography at low temperature and characterized. Under these conditions the relatively labile uridine-5′-diphosphate nucleotides of acetylglucosamine, galactose, and glucuronic acid were obtained intact, a fact that is further substantiated by the complete absence of uridine-5′-diphosphate. The occurrence of these uridine diphosphate compounds and the absence of uridine diphosphate glucose is discussed in relation to the previously postulated role of inositol as a carbohydrate storage product. A new peptide-containing nucleotide, succinoadenosine-5′-phosphosulphate (peptide), was found in the fraction which immediately follows adenosine-5′-diphosphate. The parent base of this nucleotide, succinoadenine, was also isolated. The nucleotide pattern is simpler than that reported by other investigators for rat liver and wheat.

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