THE TAC METHOD OF TRYPTIC ENZYME ACTIVITY DETERMINATION

1960 ◽  
Vol 38 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Edward Ronwin
Keyword(s):  
Enzyme Activity ◽  
Quantitative Method ◽  
Null Point ◽  
Ion Concentration ◽  
Cupric Ion ◽  
Buffer Solutions ◽  
Enzyme Substrate ◽  
Titration Procedure ◽  
Effects Of Ph

A new, practical, and quantitative method for the determination of tryptic enzyme activity is reported. The method is based on measurements of the U.V. absorption of the complex formed between cupric ion and Nα-p-toluenesulphonyl-L-arginine, the latter being the product of the reaction between the tryptic enzymes (thrombin, trypsin, plasmin) and the corresponding methyl ester. Comparisons with the null point titration procedure demonstrate the new method's superiority when dealing with enzyme–substrate reactions in concentrated buffer solutions. Under certain conditions, it could also be superior in dilute buffer situations. The effects of pH and cupric ion concentration on complex formation are discussed along with other characteristics of the U.V. absorption curves of the complex.

THE TAC METHOD OF TRYPTIC ENZYME ACTIVITY DETERMINATION

1960 ◽  
Vol 38 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Edward Ronwin
Keyword(s):  
Enzyme Activity ◽  
Quantitative Method ◽  
Null Point ◽  
Ion Concentration ◽  
Cupric Ion ◽  
Buffer Solutions ◽  
Enzyme Substrate ◽  
Titration Procedure ◽  
Effects Of Ph

A new, practical, and quantitative method for the determination of tryptic enzyme activity is reported. The method is based on measurements of the U.V. absorption of the complex formed between cupric ion and Nα-p-toluenesulphonyl-L-arginine, the latter being the product of the reaction between the tryptic enzymes (thrombin, trypsin, plasmin) and the corresponding methyl ester. Comparisons with the null point titration procedure demonstrate the new method's superiority when dealing with enzyme–substrate reactions in concentrated buffer solutions. Under certain conditions, it could also be superior in dilute buffer situations. The effects of pH and cupric ion concentration on complex formation are discussed along with other characteristics of the U.V. absorption curves of the complex.

scholarly journals THE COMBINATION OF ENZYME AND SUBSTRATE

1919 ◽  
Vol 2 (2) ◽  
pp. 113-122 ◽  
Author(s):  
John H. Northrop
Keyword(s):  
Quantitative Method ◽  
Hydrogen Ion ◽  
Ion Concentration ◽  
Albumin Solution ◽  
Hydrogen Ion Concentration ◽  
Egg Albumin ◽  
Insoluble Substrate

1. A quantitative method for the determination of pepsin is described depending on the change in conductivity of a digesting egg albumin solution. 2. The combination of pepsin with an insoluble substrate has been followed by this method. 3. The amount of pepsin removed from solution by a given weight of substrate is independent of the size of the particles of the substrate. 4. There is an optimum zone of hydrogen ion concentration for the combination of enzyme and substrate corresponding to the optimum for digestion. 5. It is suggested that the pepsin combines largely or entirely with the ionized protein.

On the colorimetric determination of hydrogen-ion concentration in soils

1923 ◽  
Vol 13 (1) ◽  
pp. 69-73 ◽  
Author(s):  
C. T. Gimingham
Keyword(s):  
Colorimetric Method ◽  
The Other ◽  
Hydrogen Ion ◽  
Ion Concentration ◽  
Colorimetric Determination ◽  
Hydrogen Ion Concentration ◽  
Electrometric Method ◽  
Buffer Solutions ◽  
Special Apparatus

Of the two available methods for the determination of hydrogen-ion concentration, the electrometric is the more fundamental and satisfactory but it requires somewhat elaborate and expensive apparatus. The colorimetric method, on the other hand, though not lacking in difficulties if accurate results are wanted, requires no special apparatus and the actual procedure is simple. It is based on the electrometric method, and involves the use of buffer solutions of known hydrogen-ion concentration determined originally electrometrically.

scholarly journals DETERMINATION OF HISTIDINE DECARBOXYLASE IN MICROGRAM SAMPLES OF TISSUE, PROPERTIES AND QUANTITATIVE HISTOCHEMISTRY OF THE ENZYME AND HISTAMINE IN THE RAT STOMACH

1967 ◽  
Vol 15 (6) ◽  
pp. 347-352 ◽  
Author(s):  
YOUNG S. KIM ◽  
DAVID GLICK
Keyword(s):  
Enzyme Activity ◽  
Histidine Decarboxylase ◽  
Preliminary Investigation ◽  
The Body ◽  
Rat Stomach ◽  
Michaelis Constant ◽  
Cell Region ◽  
Tissue Properties ◽  
Effects Of Ph

Properties of histidine decarboxylase of rat stomach were studied, including measurements of the Michaelis constant and effects of pH. Optimal conditions for determination of the enzyme were established. A method for this determination on microgram samples of tissue, e.g., microtome sections, that depends on fluorometric analysis of nanogram amounts of histamine, was elaborated and applied to a preliminary investigation of the quantitative histologic distribution of histidine decarboxylase in the body of the glandular stomach of the rat. A parallel study of the quantitative distribution of histamine was also made, and both it and the enzyme activity were predominantly concentrated in the chief cell region. From analysis of gross homogenates, the body of the stomach was found to have greater concentrations of histamine and enzyme activity than the antrum. The findings obtained were considered in relation to distribution of mast cells and acid secretion.

Fluctuations of Annual Precipitation and Water Resources Quality in Ukraine

2016 ◽  
Vol 10 (4s) ◽  
pp. 621-629
Author(s):  
Valentina Pidlisnyuk ◽  
◽  
John Harrington JR ◽  
Yulia Melnyk ◽  
Yuliya Vystavna ◽  
...  
Keyword(s):  
Water Resources ◽  
Surface Waters ◽  
Precipitation Amount ◽  
Ion Concentration ◽  
Annual Precipitation ◽  
Resource Quality ◽  
Phosphate Ion ◽  
Regional Trends

The article focuses on examining the influence of fluctuations in annual precipitation amount on the quality of surface waters. Water quality was estimated with data on BOD, COD and phosphate–ion concentration within five selected regions of Ukraine. Analysis of the precipitation data (1991 – 2010) showed different regional trends. Using the statistics, determination of the interconnection between precipitation amount and water resources quality were done. The obtained regularities and associated uncertainties can be used for prediction of changes in water resource quality and as a guide for future adaptation to possible climate change.

PROFITABILITY ANALYSIS OF TERUBUK FARMING (SACCHARUM EDULE HASSKARL) IN SUBDISTRICT TOSA DISTRICT OF EAST TIDORE OF TIDORE ISLAND

2018 ◽  
Author(s):  
Suwandi S. Sangadji
Keyword(s):  
Data Collection ◽  
Research Method ◽  
Quantitative Method ◽  
Secondary Data ◽  
Primary Data ◽  
Profitability Analysis ◽  
Sample Method ◽  
Document Review ◽  
Revenue Production

The purpose of this researchment is to ascertain how wide the farming of species Saccharun Edule Hasskarl (terubuk) in sub district Tosa, district of East Tidore of Tidore Island through the indicator of the value revenue, production and selling prices so that the farmers will achieve The Break Event Point (BEP). The research method was used a quantitative method with the number of samples of 30 people. The determination of the sample method is using the census method or involving all members of the population into a sample of researchment. The secondary data collection was done by using library literature in the form of document review and relevant references to research object while primary data collection was done by using questionnaire. The data is using equation R /C Ratio, BEP Revenue, BEP Price, and BEP Production. Therefore from the results of the researchment it can be explained that the two of the thirty farmers come through the break event point, while the other twenty-eight farmers declared having a business that worth to be develop or experiencing profit, because the R/C ratio is above 1.0 with average profit reach Rp. 989.000, - per production / farmer.

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