A SIMPLE METHOD FOR THE ASSAY OF SMALL AMOUNTS OF ANTIDIURETIC HORMONE

1959 ◽  
Vol 37 (10) ◽  
pp. 1215-1225 ◽  
Author(s):  
J. Hunter ◽  
H. Kalant ◽  
J. C. Ogilvie
Keyword(s):  
Antidiuretic Hormone ◽  
Simple Technique ◽  
Nacl Solution ◽  
Albino Rat ◽  
Simple Method ◽  
Water Load ◽  
True Value ◽  
Statistical Precision ◽  
Urine Production ◽  
Animal Material

A reliable, sensitive, and simple technique is described for the assay of antidiuretic hormone in amounts between 25 and 130 μunits. The assay animal is a conscious albino rat weighing between 150 and 250 g. On the day of assay it is tranquillized with an injection of perphenazine. Specially designed cannulae, implanted on the day before the assay, provide unimpaired access to the stomach and bladder, permitting the maintenance of hydration and continuous urine collection without disturbance of the animal. Material to be assayed is injected intravenously. Water load is maintained by manual injection of 0.5-ml quantities of 0.05% NaCl solution at intervals dependent upon the rate of urine production. A useful index for expression of the response is described, which is related to both the intensity and the duration of antidiuresis. Assay of an artificial unknown by this method gave a result within 6% of the true value, and an excellent statistical precision (λ = 0.143).

A SIMPLE METHOD FOR THE ASSAY OF SMALL AMOUNTS OF ANTIDIURETIC HORMONE

1959 ◽  
Vol 37 (1) ◽  
pp. 1215-1225
Author(s):  
J. Hunter ◽  
H. Kalant ◽  
J. C. Ogilvie
Keyword(s):  
Antidiuretic Hormone ◽  
Simple Technique ◽  
Nacl Solution ◽  
Albino Rat ◽  
Simple Method ◽  
Water Load ◽  
True Value ◽  
Statistical Precision ◽  
Urine Production ◽  
Animal Material

A reliable, sensitive, and simple technique is described for the assay of antidiuretic hormone in amounts between 25 and 130 μunits. The assay animal is a conscious albino rat weighing between 150 and 250 g. On the day of assay it is tranquillized with an injection of perphenazine. Specially designed cannulae, implanted on the day before the assay, provide unimpaired access to the stomach and bladder, permitting the maintenance of hydration and continuous urine collection without disturbance of the animal. Material to be assayed is injected intravenously. Water load is maintained by manual injection of 0.5-ml quantities of 0.05% NaCl solution at intervals dependent upon the rate of urine production. A useful index for expression of the response is described, which is related to both the intensity and the duration of antidiuresis. Assay of an artificial unknown by this method gave a result within 6% of the true value, and an excellent statistical precision (λ = 0.143).

A (Relatively) Simple Method to Visualize Single Molecules

2011 ◽  
Vol 19 (1) ◽  
pp. 8-8
Author(s):  
Stephen W. Carmichael ◽  
Julio M. Fernandez
Keyword(s):  
Fluorescence Microscopy ◽  
Simple Technique ◽  
Zero Mode ◽  
Single Molecules ◽  
Simple Method ◽  
Expensive Equipment ◽  
Freely Moving ◽  
Good For

Fluorescence microscopy can be used to study certain single molecules in solution or attached to a surface. Two conflicting challenges to overcome are: (1) to image freely moving molecules for long times and (2) to image immobilized single molecules when there is a highly fluorescent background. The fact that these two goals are inversely related is illustrated by epifluorescence, which is good for observing freely diffusing molecules but poor for detecting single molecules, whereas the reverse is true for zero-mode waveguides. Plus, these and other techniques require elaborate (read: expensive) equipment with computerized controls. Sabrina Leslie, Alexander Fields, and Adam Cohen have developed an ingenious (relatively) simple technique that can image freely moving single molecules.

Direct spectrophotometry of magnesium in serum after reaction with hexokinase and glucose-6-phosphate dehydrogenase.

1985 ◽  
Vol 31 (5) ◽  
pp. 703-705 ◽  
Author(s):  
M Tabata ◽  
T Kido ◽  
M Totani ◽  
T Murachi
Keyword(s):  
Spectrophotometric Method ◽  
Present Method ◽  
Reaction Rate ◽  
Colorimetric Method ◽  
Simple Method ◽  
True Value ◽  
A Value ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Direct Spectrophotometry

Abstract We describe a simple method for determining magnesium in serum by using hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The method is based on determination of the reaction rate of hexokinase activated by Mg2+, which participates in the hexokinase reaction as the substrate in the form of a Mg X ATP2- complex. The reaction rate is determined from the change in absorbance at 340 nm as NADPH is produced by the action of glucose-6-phosphate dehydrogenase. This simple and rapid spectrophotometric method does not require expensive instrumentation, but results correlate satisfactorily with those obtained by atomic absorption spectroscopy. Thus, the present method gives a "true" value for magnesium in serum, a value appreciably lower than that obtained by an earlier colorimetric method, the Xylidyl Blue II method (Biochem Med 7: 208-217, 1973), which lacks specificity.

A simple technique for making epidermal imprints using clear vinyl film

1985 ◽  
Vol 63 (9) ◽  
pp. 1669-1670 ◽  
Author(s):  
R. E. Redmann
Keyword(s):  
Microscopic Study ◽  
Polyvinyl Chloride ◽  
Simple Technique ◽  
Leaf Surface ◽  
Grass Species ◽  
Simple Method ◽  
Corrugated Surfaces ◽  
Chloride Film

Transparent, plasticized polyvinyl chloride film, pressed against a leaf surface and heated, produced an epidermal impression suitable for microscopic study. This rapid, simple method was tested successfully on 39 grass species, including those with corrugated surfaces and trichomes.

scholarly journals A simple technique for preparation of young red cells for transfusion from ordinary blood units

Blood ◽  
1982 ◽  
Vol 59 (4) ◽  
pp. 865-868
Author(s):  
JH Graziano ◽  
S Piomelli ◽  
C Seaman ◽  
T Wang ◽  
AR Cohen ◽  
...  
Keyword(s):  
Simple Technique ◽  
Thalassemia Major ◽  
Red Cells ◽  
Gradient System ◽  
Cell Processor ◽  
Survival Times ◽  
Pyruvate Kinase Activity ◽  
Simple Method ◽  
In Vitro Measurements

We have developed a rapid and simple method for the isolation of relatively young cells from ordinary fresh or previously frozen blood units, using the IBM-2991 Blood Cell Processor. Based on in vitro measurements of red cell pyruvate kinase activity, the resolution of the method is excellent, comparable to that of the stractan discontinuous gradient system. In splenectomized patients with thalassemia major, the administration of the “youngest” 50% of blood units yielded 51Cr survival times of 40 and 42 days for fresh packed red cells and 41 and 43 days for previously frozen red cells.

scholarly journals A simple method for fabricating custom sectional impression trays for making definitive impressions in patients with microstomia

2012 ◽  
Vol 06 (03) ◽  
pp. 244-247 ◽  
Author(s):  
Vinay Chila Bachhav ◽  
Meena Ajay Aras
Keyword(s):  
Simple Technique ◽  
Mouth Opening ◽  
Complete Denture ◽  
Simple Method

ABSTRACTObjectives: A maximum mouth opening that is smaller than the size of a complete denture can make prosthetic treatment challenging. This article describes a simple technique used to fabricate maxillary and mandibular custom sectional impression trays for making definitive impressions in patients with microstomia. (Eur J Dent 2012;6:244-247)

scholarly journals Application of the Simple Verification Method to Estimate the Weather at Makassar Maritime Station, Indonesia

2021 ◽  
Vol 18 (18) ◽  
Author(s):  
Didiharyono DIDIHARYONO ◽  
Giarno GIARNO
Keyword(s):  
Evaluation Method ◽  
Weather Prediction ◽  
Weather Forecast ◽  
Process Performance ◽  
Simple Method ◽  
Verification Method ◽  
Weather Forecasts ◽  
The Public ◽  
True Value

Verification is used to measure the quality of a weather prediction, improve process performance, and measure the value of weather estimation. Initially, weather verification developed after Finley published his paper on the verification of tornado events. The type of data, objectives, and scale can make a different method in using weather verification. If there are some parameters that can be predicted, a simple question is consequently often asked by the public: how accurate are weather forecasts? Nowadays, the public wants a simple answer in 1 value that is presented quantitatively. The aim of the research is to develop a simple method that can answer the accuracy of weather prediction in a value that is easily understood by the public. Practically, validation comparing between prediction and observation parameters is divided into 2, namely dichotomous and comparing the values. This research tries to combine all weather prediction variables into a dichotomous variable with a threshold. Moreover, this technique is tested on weather predictions for the port of Makassar over a year. The results show that a certain threshold can be used to change the weather variable to be dichotomous. With the application of this method, forecast accuracy and suitability between the predicted parameters can be obtained. Moreover, the weather forecast issued by the Makassar Maritime Station shows the average true value of the forecast to be 69.1 %, and then the capabilities vary by forecasters, which range from 61 to 79 %. HIGHLIGHTS Weather forecast verification is used to measure the quality of a weather forecast, improve process performance, and the value of weather forecasts The character of the weather variables and their predictions is unique and influences the type of evaluation method To facilitate the public's assessment of the accuracy of weather predictions, it is necessary to combine weather prediction evaluation methods in one value Using the tolerance threshold whether a deviant prediction is used to combine various weather predictive variables Average true value of the forecast is 69.1 % and the different capabilities of each forecaster, which range from 61 to 79 % GRAPHICAL ABSTRACT

PITUITARY ADENOMA ASSOCIATED WITH INAPPROPRIATE ANTIDIURETIC HORMONE SECRETION

1974 ◽  
Vol 76 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Kamal C. Greiss ◽  
Arnold M. Moses ◽  
Dorothy T. Krieger
Keyword(s):  
Pituitary Adenoma ◽  
Antidiuretic Hormone ◽  
Hormone Secretion ◽  
Ethanol Ingestion ◽  
Water Load ◽  
Inappropriate Antidiuretic Hormone Secretion ◽  
Salt Wasting ◽  
Antidiuretic Hormone Secretion ◽  
Chromophobe Adenoma ◽  
Suprasellar Extension

ABSTRACT A 50 year old male with a chromophobe adenoma with suprasellar extension manifested hyponatraemia, plasma hypoosmolality, renal salt wasting and inability to excrete dilute urine after a standard water load. The patient exhibited no evidence of thyroid or adrenal insufficiency. The inability to excrete a water load was not corrected by prior cortisone administration but was partially corrected after ethanol ingestion. A measurable inappropriate amount of arginine vasopressin was excreted in the presence of sustained hydration. It is suggested that inappropriate production of antidiuretic hormone either by the adenoma, or secondary to hypothalamic involvement by the suprasellar extension of the tumour was responsible for the observed findings.

A Simple Technique for Applying Local Splintage to the Fingers

1999 ◽  
Vol 24 (5) ◽  
pp. 610-611
Author(s):  
J. A. PEREIRA ◽  
F. SCHONAUER ◽  
H. J. C. R. BELCHER
Keyword(s):  
Simple Technique ◽  
Standard Technique ◽  
Simple Method

We report a simple method of splinting the interphalangeal joints that we have employed as our standard technique without complication.

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