CORRELATED STUDIES OF STEROID FORMATION IN VITRO, ASCORBIC ACID CONCENTRATIONS, AND CYTOLOGICAL CHANGES IN ADRENAL GLANDS OF RATS GIVEN HISTAMINE

1958 ◽  
Vol 36 (6) ◽  
pp. 571-574 ◽  
Author(s):  
E. Schönbaum ◽  
W. G. Bruce Casselman
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Glands ◽  
Cytological Changes

In the adrenal glands of rats given histamine, there are significant correlations between the increase in steroid formation in vitro, and the depletion of ascorbic acid or cytological changes in Z. fasciculata and Z. reticularis indicative of stimulation.

CORRELATED STUDIES OF STEROID FORMATION IN VITRO, ASCORBIC ACID CONCENTRATIONS, AND CYTOLOGICAL CHANGES IN ADRENAL GLANDS OF RATS GIVEN HISTAMINE

1958 ◽  
Vol 36 (1) ◽  
pp. 571-574
Author(s):  
E. Schönbaum ◽  
W. G. Bruce Casselman
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Glands ◽  
Cytological Changes

In the adrenal glands of rats given histamine, there are significant correlations between the increase in steroid formation in vitro, and the depletion of ascorbic acid or cytological changes in Z. fasciculata and Z. reticularis indicative of stimulation.

“IN VIVO” AND “IN VITRO” EFFECT OF ASCORBIC ACID AND ACTH ON THE RAT ADRENAL CORTEX

1952 ◽  
Vol 30 (3) ◽  
pp. 157-162
Author(s):  
A. DesMarais ◽  
J. Leblanc
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Cortex ◽  
Adrenal Glands ◽  
Secretory Activity ◽  
In Vitro Experiments ◽  
Hypophysectomized Rats ◽  
Vitro Effect ◽  
Histochemical Examination

Histochemical examination of adrenal glands of hypophysectomized rats given both ascorbic acid and ACTH showed an enlargement of the cortex and a decrease of sudanophilic substances, as compared to adrenals of hypophysectomized rats receiving ACTH alone. “In vitro” experiments on incubated slices of adrenal glands have shown that ascorbic acid and ACTH have a synergistic effect on the secretory activity of the cells of the adrenal cortex.

STUDIES ON THE TIME COURSE OF THE RESPONSE OF THE ADRENAL CORTEX TO HISTAMINE AND COLD

1959 ◽  
Vol 37 (3) ◽  
pp. 399-404 ◽  
Author(s):  
E. Schönbaum ◽  
W. G. Bruce Casselman ◽  
Rosemary E. Large
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Cortex ◽  
Time Course ◽  
Adrenal Glands ◽  
Transient Elevation

Rats were injected with histamine or exposed to cold. In either case there was a transient elevation in the output of corticosteroids by the excised adrenal glands in vitro but a prolonged fall in adrenal ascorbic acid. The return of steroid formation to pre-exposure or even lower rates within three hours was not accompanied by any further change of the concentration of adrenal ascorbic acid.

STUDIES ON THE TIME COURSE OF THE RESPONSE OF THE ADRENAL CORTEX TO HISTAMINE AND COLD

1959 ◽  
Vol 37 (1) ◽  
pp. 399-404
Author(s):  
E. Schönbaum ◽  
W. G. Bruce Casselman ◽  
Rosemary E. Large
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Cortex ◽  
Time Course ◽  
Adrenal Glands ◽  
Transient Elevation

Rats were injected with histamine or exposed to cold. In either case there was a transient elevation in the output of corticosteroids by the excised adrenal glands in vitro but a prolonged fall in adrenal ascorbic acid. The return of steroid formation to pre-exposure or even lower rates within three hours was not accompanied by any further change of the concentration of adrenal ascorbic acid.

In vitro Uptake of ascorbic acid-1-14C by adrenal glands of different species of animals

1968 ◽  
Vol 11 (2) ◽  
pp. 332-337 ◽  
Author(s):  
Alejandro F. de Nicola ◽  
Michael Clayman ◽  
Rose M. Johnstone
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Glands ◽  
In Vitro Uptake

Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

1990 ◽  
Vol 48 (3) ◽  
pp. 290-291
Author(s):  
Gustav Ofosu
Keyword(s):  
Mammalian Cells ◽  
Antitumor Agent ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Limiting Factor ◽  
Sarcoma 180 ◽  
Electron Microscopic ◽  
Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

CONTRIBUTION TO THE EXISTENCE OF REGULATORY MECHANISMS AT THE ADRENAL LEVEL

1972 ◽  
Vol 70 (4) ◽  
pp. 741-757
Author(s):  
Otto Linèt
Keyword(s):  
Orotic Acid ◽  
Adrenal Glands ◽  
Actinomycin D ◽  
Delay Period ◽  
Regulatory Mechanisms ◽  
Leucine Incorporation ◽  
Total Period ◽  
Free Media

ABSTRACT Rat adrenal glands atrophied by the administration of cortisol acetate in vivo were used as a model for the study of early metabolic processes occurring in vitro. Atrophied adrenals incubated in the presence of 14C-leucine incorporated subnormal quantities of this amino acid per mg of protein for the first 120 min. When the incubation lasted for a total period of 180 or 240 min a supranormal rise in the 14C-leucine incorporation was observed. Similar changes occurred with some delay with regard to corticosterone production as expressed per 100 mg of tissue. No differences in 14C-leucine incorporation were observed between the control and atrophied adrenals in vivo. Homogenates from atrophied glands incorporated 14C-leucine to a greater extent than the control homogenates. The in vitro incorporation of 14C-orotic acid into the RNA was also higher in atrophied adrenals. The in vitro use of actinomycin D, cycloheximide and amphenone indicated that corticosterone production depended on the incorporation of 14C-leucine. The addition of cortisol to the incubation media markedly decreased the enhancement of 14C-lysine incorporation into the protein of atrophied adrenals. These, as well as additional results suggest rebound phenomena: once atrophic adrenals are transferred to cortisol-free media, reparative processes begin after a delay period. Such phenomena seem to be mediated by regulatory mechanisms at the adrenal level.

In vitro and in vivo reduction of erythrocyte sorbitol by ascorbic acid

Diabetes ◽  
1989 ◽  
Vol 38 (8) ◽  
pp. 1036-1041 ◽  
Author(s):  
J. A. Vinson ◽  
M. E. Staretz ◽  
P. Bose ◽  
H. M. Kassm ◽  
B. S. Basalyga
Keyword(s):  
Ascorbic Acid

IMPACT OF SORBITOL- INDUCED OSMOTIC STRESS ON SOME BIOCHEMICAL TRAITS OF POTATO IN VITRO

2020 ◽  
Vol 51 (4) ◽  
pp. 1038-1047
Author(s):  
Mawia & et al.
Keyword(s):  
Ascorbic Acid ◽  
Osmotic Stress ◽  
Cytoplasmic Membrane ◽  
Genotypic Variation ◽  
Membrane Damage ◽  
Membrane Integrity ◽  
Culture Collection ◽  
Nutritive Medium ◽  
Starting Point

This study had as principal objective identification of osmotic-tolerant potato genotypes by using "in vitro" tissue culture and sorbitol as a stimulating agent, to induce water stress, which was added to the  culture nutritive medium in different concentration (0,50, 110, 220, 330 and 440 mM).  The starting point was represented by plantlets culture collection, belonging to eleven potato genotypes: Barcelona, Nectar, Alison, Jelly, Malice, Nazca, Toronto, Farida, Fabulla, Colomba and Spunta. Plantlets were multiplied between two internodes to obtain microcuttings (in sterile condition), which were inoculated on medium. Sorbitol-induced osmotic stress caused a significant reduction in the ascorbic acid, while the concentration of proline, H2O2 and solutes leakage increased compared with the control. Increased the proline content prevented lipid peroxidation, which played a pivotal role in the maintenance of membrane integrity under osmotic stress conditions. The extent of the cytoplasmic membrane damage depends on osmotic stress severity and the genotypic variation in the maintenance of membranes stability was highly associated with the ability of producing more amounts of osmoprotectants (proline) and the non-enzymic antioxidant ascorbic acid in response to osmotic stress level. The results showed that the genotypes Jelly, Nectar, Allison, Toronto, and Colomba are classified as highly osmotic stress tolerant genotypes, while the genotypes Nazca and Farida are classified as osmotic stress susceptible ones.

Sign in / Sign up

Export Citation Format

Share Document