THE BIOLOGICAL ASSAY OF CHORIONIC GONADOTROPHIN AND OF THE GONADOTROPHIN OF PREGNANT MARE'S SERUM USING DIETARY ANOESTROUS ADULT RATS

1957 ◽  
Vol 35 (11) ◽  
pp. 889-896
Author(s):  
L. I. Pugsley
Keyword(s):  
Convenient Method ◽  
Anterior Pituitary ◽  
Pharmaceutical Preparations ◽  
Chorionic Gonadotrophin ◽  
International Standards ◽  
Biological Assay ◽  
Test Animal ◽  
Satisfactory Solution ◽  
Adult Rats ◽  
Special Diets

A satisfactory and convenient method for the biological assay of pharmaceutical preparations of chorionic and pregnant mare's serum gonadotrophin using adult dietary anoestrous rats has been described, and data are presented showing the variations in slope and RD50 during a number of years of experience in the use of the method employing the International Standards of these hormones. The findings of Heard and Winton have been fully confirmed as to the reliability and precision of the method. The suggestion made by Collip, in his early studies, that a satisfactory solution to the biological assay of anterior-pituitary-like hormones may be arrived at by the use of special diets to render the test animal acyclic has been accomplished.

THE BIOLOGICAL ASSAY OF CHORIONIC GONADOTROPHIN AND OF THE GONADOTROPHIN OF PREGNANT MARE'S SERUM USING DIETARY ANOESTROUS ADULT RATS

1957 ◽  
Vol 35 (1) ◽  
pp. 889-896
Author(s):  
L. I. Pugsley
Keyword(s):  
Convenient Method ◽  
Anterior Pituitary ◽  
Pharmaceutical Preparations ◽  
Chorionic Gonadotrophin ◽  
International Standards ◽  
Biological Assay ◽  
Test Animal ◽  
Satisfactory Solution ◽  
Adult Rats ◽  
Special Diets

A satisfactory and convenient method for the biological assay of pharmaceutical preparations of chorionic and pregnant mare's serum gonadotrophin using adult dietary anoestrous rats has been described, and data are presented showing the variations in slope and RD50 during a number of years of experience in the use of the method employing the International Standards of these hormones. The findings of Heard and Winton have been fully confirmed as to the reliability and precision of the method. The suggestion made by Collip, in his early studies, that a satisfactory solution to the biological assay of anterior-pituitary-like hormones may be arrived at by the use of special diets to render the test animal acyclic has been accomplished.

NGF and BDNF in the anterior pituitary lobe of adult rats

1997 ◽  
Vol 49 (3) ◽  
pp. 355-363 ◽  
Author(s):  
Veit H. H�pker ◽  
Marie-Claude Amoureux ◽  
Silvio Varon
Keyword(s):  
Anterior Pituitary ◽  
Adult Rats

INHIBITION BY MELATONIN OF THE PITUITARY RESPONSE TO LUTEINIZING HORMONE RELEASING HORMONE IN VIVO

1978 ◽  
Vol 76 (3) ◽  
pp. 487-491 ◽  
Author(s):  
K. YAMASHITA ◽  
M. MIENO ◽  
T. SHIMIZU ◽  
ER. YAMASHITA
Keyword(s):  
Luteinizing Hormone ◽  
Carotid Artery ◽  
Anterior Pituitary ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Lh Rh ◽  
Lh Secretion

The rate of secretion of 17-oxosteroids by the testes of anaesthetized dogs in vivo was used as an index of LH secretion. Intracarotid injection of luteinizing hormone releasing hormone (LH-RH, 1, 5 or 10 μg/kg body wt) resulted in an increase in the testicular 17-oxosteroid secretion which was roughly proportional to the dose administered and which reached a maximum 60 min after the injection. Testicular output of 17-oxosteroids was unaffected by administration of melatonin (10 or 100 μg/kg body wt) into the carotid artery. When LH-RH (5 μg/kg) was injected into the carotid artery 3 h after intracarotid injection of melatonin (10 or 100 μg/kg), the testicular response to LH-RH was considerably diminished. Pretreatment with melatonin (100 μg/kg) did not alter the testicular response to human chorionic gonadotrophin (20 i.u./kg body wt) given i.v. It is concluded that melatonin may act directly on the anterior pituitary gland in dogs to inhibit the LH-RH-induced release of LH.

Expression of retinaldehyde dehydrogenase 1 in the anterior pituitary glands of adult rats

2007 ◽  
Vol 329 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Ken Fujiwara ◽  
Motoshi Kikuchi ◽  
Shu Takigami ◽  
Tom Kouki ◽  
Takashi Yashiro
Keyword(s):  
Anterior Pituitary ◽  
Adult Rats ◽  
Pituitary Glands ◽  
Retinaldehyde Dehydrogenase

THE SENSITIVITY OF ADRENALECTOMIZED RATS TO GONADOTROPHINS

1954 ◽  
Vol 11 (4) ◽  
pp. 359-376 ◽  
Author(s):  
ANITA M. MANDL
Keyword(s):  
Chorionic Gonadotrophin ◽  
Female Rats ◽  
Corpora Lutea ◽  
Surgical Trauma ◽  
Normal Reaction ◽  
Normal Response ◽  
Immature Female ◽  
Adult Rats ◽  
Immature Rats ◽  
Adrenalectomized Rats

SUMMARY The sensitivity of adrenalectomized, control-operated and unoperated rats to pregnant mare serum (PMS) and chorionic gonadotrophin (CG) has been studied. A total of 638 mature and immature female rats was used. The ovaries of adrenalectomized rats were found to contain fewer large follicles and corpora lutea than those of control-operated litter-mates, and the slight ovarian hypertrophy which occurs after surgical trauma was found to be due to an increase in the number of Graafian follicles and corpora lutea. Further experiment showed that, as judged by the weight of the ovaries, adrenalectomy reduces the ovarian reaction to injected PMS (10 i.u./day) in both adult and immature rats. Replacement therapy with DCA (1 mg/day) failed to re-establish the normal response in adults. Treatment with cortisone (1 mg/day) restored the normal reaction in both adult and immature adrenalectomized rats. Adrenalectomized adult rats responded to injected CG (10 i.u./day) as vigorously as their operated and unoperated litter-mates. On the other hand, immature adrenalectomized animals did not respond fully to CG. Treatment with cortisone again fully restored the normal reaction.

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